Cell leading into microwell array by using negative dielectrophoresis

In order to accelerate cell leading into microwells on single cell based microwell array system, negative dielectrophoresis was employed. Gold thin film was formed onto PDMS microwell array sheet and high frequency sinusoidal voltage was applied between the gold film and ITO electrode which was above microwell array. Optimal conditions for negative dielectrophoresis were 100 MHz and 3 V in this system. Implanted ratio (ratio of cells in wells comparing with total cells in the measured image) was 63.8%, over 4.5 fold higher than without voltage application. The application of optimal sinusoidal voltage gave no damage on animal cells.