Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD

Mineyuki Mizuguchi; Takayuki Obita; Asagi Kajiyama; Yuki Kozakai; Tsuyoshi Nakai; Yuko Nabeshima; Hitoshi Okazawa

doi:10.1002/1873-3468.12256

Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD

Mineyuki Mizuguchi^*, Takayuki Obita, Asagi Kajiyama, Yuki Kozakai, Tsuyoshi Nakai, Yuko Nabeshima, Hitoshi Okazawa

^*この論文の責任著者

構造生物学研究室

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

8 被引用数 (Scopus)

抄録

Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein composed of a small folded WW domain and a long disordered region. PQBP1 binds to spliceosomal proteins WBP11 and U5-15kD through its N-terminal WW domain and C-terminal region, respectively. Here, we reveal that the binding between PQBP1 and WBP11 reduces the binding affinity between PQBP1 and U5-15kD. Our results suggest that the interaction between PQBP1 and WBP11 negatively modulates the U5-15kD binding of PQBP1 by an allosteric mechanism.

本文言語	英語
ページ（範囲）	2221-2231
ページ数	11
ジャーナル	FEBS Letters
DOI	https://doi.org/10.1002/1873-3468.12256
出版ステータス	出版済み - 2016/07/01

ASJC Scopus 主題領域

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

文献へのアクセス

10.1002/1873-3468.12256

引用スタイル

@article{a7cd0a4d27b548b1b62c1fd42f1b6aa8,

title = "Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD",

abstract = "Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein composed of a small folded WW domain and a long disordered region. PQBP1 binds to spliceosomal proteins WBP11 and U5-15kD through its N-terminal WW domain and C-terminal region, respectively. Here, we reveal that the binding between PQBP1 and WBP11 reduces the binding affinity between PQBP1 and U5-15kD. Our results suggest that the interaction between PQBP1 and WBP11 negatively modulates the U5-15kD binding of PQBP1 by an allosteric mechanism.",

keywords = "allosteric mechanism, interaction, intrinsically disordered protein",

author = "Mineyuki Mizuguchi and Takayuki Obita and Asagi Kajiyama and Yuki Kozakai and Tsuyoshi Nakai and Yuko Nabeshima and Hitoshi Okazawa",

note = "Publisher Copyright: {\textcopyright} 2016 Federation of European Biochemical Societies",

year = "2016",

month = jul,

day = "1",

doi = "10.1002/1873-3468.12256",

language = "英語",

pages = "2221--2231",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD

AU - Mizuguchi, Mineyuki

AU - Obita, Takayuki

AU - Kajiyama, Asagi

AU - Kozakai, Yuki

AU - Nakai, Tsuyoshi

AU - Nabeshima, Yuko

AU - Okazawa, Hitoshi

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein composed of a small folded WW domain and a long disordered region. PQBP1 binds to spliceosomal proteins WBP11 and U5-15kD through its N-terminal WW domain and C-terminal region, respectively. Here, we reveal that the binding between PQBP1 and WBP11 reduces the binding affinity between PQBP1 and U5-15kD. Our results suggest that the interaction between PQBP1 and WBP11 negatively modulates the U5-15kD binding of PQBP1 by an allosteric mechanism.

AB - Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein composed of a small folded WW domain and a long disordered region. PQBP1 binds to spliceosomal proteins WBP11 and U5-15kD through its N-terminal WW domain and C-terminal region, respectively. Here, we reveal that the binding between PQBP1 and WBP11 reduces the binding affinity between PQBP1 and U5-15kD. Our results suggest that the interaction between PQBP1 and WBP11 negatively modulates the U5-15kD binding of PQBP1 by an allosteric mechanism.

KW - allosteric mechanism

KW - interaction

KW - intrinsically disordered protein

UR - http://www.scopus.com/inward/record.url?scp=84979609067&partnerID=8YFLogxK

U2 - 10.1002/1873-3468.12256

DO - 10.1002/1873-3468.12256

M3 - 学術論文

C2 - 27314904

AN - SCOPUS:84979609067

SN - 0014-5793

SP - 2221

EP - 2231

JO - FEBS Letters

JF - FEBS Letters

ER -

Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD

抄録

ASJC Scopus 主題領域

文献へのアクセス

フィンガープリント

引用スタイル