Aberrant methylation of H19-DMR acquired after implantation was dissimilar in soma versus placenta of patients with Beckwith-Wiedemann syndrome

Ken Higashimoto, Kazuhiko Nakabayashi, Hitomi Yatsuki, Hokuto Yoshinaga, Kosuke Jozaki, Junichiro Okada, Yoriko Watanabe, Aiko Aoki, Arihiro Shiozaki, Shigeru Saito, Kayoko Koide, Tsunehiro Mukai, Kenichiro Hata, Hidenobu Soejima*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

8 被引用数 (Scopus)

抄録

Gain of methylation (GOM) at the H19-differentially methylated region (H19-DMR) is one of several causative alterations in Beckwith-Wiedemann syndrome (BWS), an imprinting-related disorder. In most patients with epigenetic changes at H19-DMR, the timing of and mechanism mediating GOM is unknown. To clarify this, we analyzed methylation at the imprinting control regions of somatic tissues and the placenta from two unrelated, naturally conceived patients with sporadic BWS. Maternal H19-DMR was abnormally and variably hypermethylated in both patients, indicating epigenetic mosaicism. Aberrant methylation levels were consistently lower in placenta than in blood and skin. Mosaic and discordant methylation strongly suggested that aberrant hypermethylation occurred after implantation, when genome-wide de novo methylation normally occurs. We expect aberrant de novo hypermethylation of H19-DMR happens to a greater extent in embryos than in placentas, as this is normally the case for de novo methylation. In addition, of 16 primary imprinted DMRs analyzed, only H19-DMR was aberrantly methylated, except for NNAT DMR in the placental chorangioma of Patient 2. To our knowledge, these are the first data suggesting when GOM of H19-DMR occurs.

本文言語英語
ページ(範囲)1670-1675
ページ数6
ジャーナルAmerican Journal of Medical Genetics, Part A
158 A
7
DOI
出版ステータス出版済み - 2012/07

ASJC Scopus 主題領域

  • 遺伝学
  • 遺伝学(臨床)

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