抄録
Antigen-specific human monoclonal antibodies (mAbs) are key candidates for therapeutic agents. However, the availability of a suitable screening system for antigen-specific antibody-secreting cells (ASCs) is limited in humans. Here we present a unique method for detecting individual ASCs using microwell array chips, which enables the analysis of live cells on a single-cell basis and offers a rapid, efficient and high-throughput (up to 234,000 individual cells) system for identifying and recovering objective ASCs. We applied the system to detect and retrieve ASCs for hepatitis B virus and influenza viruses from human peripheral blood lymphocytes and produced human mAbs with virus-neutralizing activities within a week. Furthermore, we show that the system is useful for detecting ASCs for multiple antigens as well as for selection of ASCs secreting high-affinity antibodies on a chip. Our method can open the way for the generation of therapeutic antibodies for individual patients.
本文言語 | 英語 |
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ページ(範囲) | 1088-1092 |
ページ数 | 5 |
ジャーナル | Nature Medicine |
巻 | 15 |
号 | 9 |
DOI | |
出版ステータス | 出版済み - 2009/09 |
ASJC Scopus 主題領域
- 生化学、遺伝学、分子生物学一般