A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood

Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, Sachiko Kondo, Koshi Kinoshita, Shinichi Kadowaki, Kazuo Takahashi, Toshiro Sugiyama, Hiroyuki Kishi*, Atsushi Muraguchi

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

242 被引用数 (Scopus)

抄録

Antigen-specific human monoclonal antibodies (mAbs) are key candidates for therapeutic agents. However, the availability of a suitable screening system for antigen-specific antibody-secreting cells (ASCs) is limited in humans. Here we present a unique method for detecting individual ASCs using microwell array chips, which enables the analysis of live cells on a single-cell basis and offers a rapid, efficient and high-throughput (up to 234,000 individual cells) system for identifying and recovering objective ASCs. We applied the system to detect and retrieve ASCs for hepatitis B virus and influenza viruses from human peripheral blood lymphocytes and produced human mAbs with virus-neutralizing activities within a week. Furthermore, we show that the system is useful for detecting ASCs for multiple antigens as well as for selection of ASCs secreting high-affinity antibodies on a chip. Our method can open the way for the generation of therapeutic antibodies for individual patients.

本文言語英語
ページ(範囲)1088-1092
ページ数5
ジャーナルNature Medicine
15
9
DOI
出版ステータス出版済み - 2009/09

ASJC Scopus 主題領域

  • 生化学、遺伝学、分子生物学一般

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