@article{bf38daec7e6f4511b6c01297353e028a,
title = "A novel compound from Hedysarum polybotrys",
abstract = "A polyhydroxyl constituent (1), named as polybotrin, along with two known compounds, were isolated from the roots of Hedysarum polybotrys. Their structures were identified based on chemical and spectroscopic evidence.",
keywords = "Hedysarum polybotrys, Polybotrys, Polyhydroxyl constituent",
author = "Kun Zou and Katsuko Komatsu and Shu Zhu",
note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (B), No.14406030 in 2002–2004 and No.17406004 in 2005–2007 from the Japan Society for the Promotion of Science, and by the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan. Funding Information: 3D structure of compound 1. The distances between atoms were calculated by CS Chem3D Pro.3. Experimental3.1 General experimental proceduresOptical rotations were measured using a Jasco DIP-360 digital polarimeter. IR spectra were measured in KBr using a Jasco FT/IR-230 infrared spectrometer. 1H NMR, 13C NMR and 2D NMR spectra were obtained with a JEOL JNA-LA 400WB-FT spectrometer (1H, 400 MHz; 13C, 100 MHz), the chemical shifts being represented as with TMS as an internal standard. The FAB mass spectra (positive and negative modes) were taken on a JEOL JMS-AX505HAD mass analyser, MALDI TOF mass spectra on a Bruker Daltonics Autoflex-T1 mass analyser, and EI mass spectra on a JEOL JMS-GCMATE mass analyser. HPLC was performed on a Jasco PU-1587 intelligent preparative pump equipped with a Jasco UV-1575 intelligent UV/Vis detector and a Jasco 807-IT integrator; column, YMC-Pack ODS-AQ (S-5 νm, 12 nm, 250 £ 20 mm i.d.); flow rate, 8.0 ml/min; detection, UV at 220 nm.3.2 Plant materialDried root of Hedysarum polybotrys was purchased from Tochimoto Tenkaido Co. Ltd in March 2003. A voucher specimen (TMPW No. 22194) is deposited in the Museum of Materia Medica, Institute of Natural Medicine, University of Toyama (TMPW), Toyama, Japan.3.3 Extraction and isolationAir-dried sheeted root (2.0 kg) was extracted with methanol. The methanol residue (668 g) was suspended in water, and then extracted with n-hexane, ethyl acetate and n-butanol,respectively. The n-butanol soluble part was subjected to silica gel column chromatography eluted with a gradient solvent system of chloroform and methanol (10:1 ! 7:3) to give rise to 26 fractions. Fraction 11 was subjected to chromatography over an RP C-18 medium pressure column (Lobarw pre-packed column), eluted with a gradient solvent system of δ: 4.01 (2H, q, J ¼ 7.08 Hz), 3.35 (2H, s), 1.97 (3H, s), 1.15 (3H, t, J ¼ 7.08 Hz). 13C NMR (125 MHz, CDCl3) δ: 170.4, 72.3, 60.3, 59.7, 20.7, 18.7.AcknowledgementsThis work was supported by a Grant-in-Aid for Scientific Research (B), No.14406030 in 2002–2004 and No.17406004 in 2005–2007 from the Japan Society for the Promotion of Science, and by the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan.",
year = "2007",
month = jul,
doi = "10.1080/10286020600604385",
language = "英語",
volume = "9",
pages = "481--485",
journal = "Journal of Asian Natural Products Research",
issn = "1028-6020",
publisher = "Taylor and Francis Ltd.",
number = "5",
}