3D video analysis of the novel object recognition test in rats

Jumpei Matsumoto, Takashi Uehara, Susumu Urakawa, Yusaku Takamura, Tomiki Sumiyoshi, Michio Suzuki, Taketoshi Ono, Hisao Nishijo*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

18 被引用数 (Scopus)

抄録

The novel object recognition (NOR) test has been widely used to test memory function. We developed a 3D computerized video analysis system that estimates nose contact with an object in Long Evans rats to analyze object exploration during NOR tests. The results indicate that the 3D system reproducibly and accurately scores the NOR test. Furthermore, the 3D system captures a 3D trajectory of the nose during object exploration, enabling detailed analyses of spatiotemporal patterns of object exploration. The 3D trajectory analysis revealed a specific pattern of object exploration in the sample phase of the NOR test: normal rats first explored the lower parts of objects and then gradually explored the upper parts. A systematic injection of MK-801 suppressed changes in these exploration patterns. The results, along with those of previous studies, suggest that the changes in the exploration patterns reflect neophobia to a novel object and/or changes from spatial learning to object learning. These results demonstrate that the 3D tracking system is useful not only for detailed scoring of animal behaviors but also for investigation of characteristic spatiotemporal patterns of object exploration. The system has the potential to facilitate future investigation of neural mechanisms underlying object exploration that result from dynamic and complex brain activity.

本文言語英語
ページ(範囲)16-24
ページ数9
ジャーナルBehavioural Brain Research
272
DOI
出版ステータス出版済み - 2014/10/01

ASJC Scopus 主題領域

  • 行動神経科学

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