Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD

  • Takuji Nakamura (作成者)
  • Tomomi Satoh-Nakamura (作成者)
  • Akio Nakajima (作成者)
  • Takafumi Kawanami (作成者)
  • Tomoyuki Sakai (作成者)
  • Yoshimasa Fujita (作成者)
  • Haruka Iwao (作成者)
  • Miyuki Miki (作成者)
  • Yasufumi Masaki (作成者)
  • Toshiro Okazaki (作成者)
  • Yasuhito Ishigaki (作成者)
  • Mitsuhiro Kawano (作成者)
  • Kazunori Yamada (作成者)
  • Shoko Matsui (作成者)
  • Takako Saeki (作成者)
  • Terumi Kamisawa (作成者)
  • Motohisa Yamamoto (作成者)
  • Hideaki Hamano (作成者)
  • Tomoki Origuchi (作成者)
  • Shintaro Hirata (作成者)
  • Yoshiya Tanaka (作成者)
  • Hiroto Tsuboi (作成者)
  • Takayuki Sumida (作成者)
  • Kazuichi Okazaki (作成者)
  • Masao Tanaka (作成者)
  • Tsutomu Chiba (作成者)
  • Tsuneyo Mimori (寄稿者)
  • Hisanori Umehara (作成者)

データセット

説明

Background: IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and tissue infiltration by IgG4-positive plasma cells. The pathogenesis of this disease is not clear. Transcriptome analysis was performed to identify genes over- and under-expressed in patients with IgG4-RD. Method: DNA microarray analysis was performed using RNA from peripheral blood mononuclear cells of two patients with IgG4-RD and four healthy individuals. Genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy were identified. Four genes related to innate immunity such as transcobalamin I (TCN1), secretory leukocyte peptidase inhibitor (SLPI), bactericidal/permeability-increasing protein (BPI) and lactotransferrin (LTF) were assessed by real-time PCR in 15 IgG4-RD patients and 13 healthy individuals. Result: DNA microarray analysis identified 30 genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy. Real-time RT-PCR showed that the levels of mRNAs encoding TCNI and SLPI, except for BPI and LTF, were significantly lower in patients with IgG4-RD than in healthy people. The levels of all four mRNAs in patients with IgG4-RD were significantly increased after steroid treatment. Conclusion: These results indicate that reduction in expression of innate immunity-related genes may participate in the pathogenesis of IgG4-RD that steroid treatment may rectify impaired innate immunity as well as acquired immunity.
利用可能になった日2019
出版社Taylor & Francis

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