Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis

  • Nozomu Yoshioka (作成者)
  • Shinji Miyata (作成者)
  • Atsushi Tamada (作成者)
  • Yumi Watanabe (作成者)
  • Asami Kawasaki (作成者)
  • Hiroshi Kitagawa (作成者)
  • Keizo Takao (作成者)
  • Tsuyoshi Miyakawa (作成者)
  • Kosei Takeuchi (寄稿者)
  • Michihiro Igarashia (作成者)

データセット

説明

Abstract Chondroitin sulfate (CS) is an important glycosaminoglycan and is mainly found in the extracellular matrix as CS proteoglycans. In the brain, CS proteoglycans are highly concentrated in perineuronal nets (PNNs), which surround synapses and modulate their functions. To investigate the importance of CS, we produced and precisely examined mice that were deficient in the CS synthesizing enzyme, CSGalNAcT1 (T1KO). Biochemical analysis of T1KO revealed that loss of this enzyme reduced the amount of CS by approximately 50% in various brain regions. The amount of CS in PNNs was also diminished in T1KO compared to wild-type mice, although the amount of a major CS proteoglycan core protein, aggrecan, was not changed. In T1KO, we observed abnormalities in several behavioral tests, including the open-field test, acoustic startle response, and social preference. These results suggest that T1 is important for plasticity, probably due to regulation of CS-dependent PNNs, and that T1KO is a good model for investigation of PNNs.
利用可能になった日2017
出版社figshare

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