W-7 specifically inhibits insulin-induced increase in glucose transport

O. Ishibashi; M. Kobayashi; T. Sasaoka; M. Sugibayashi; Y. Shigeta

doi:10.1016/0168-8227(89)90114-9

W-7 specifically inhibits insulin-induced increase in glucose transport

O. Ishibashi, M. Kobayashi^*, T. Sasaoka, M. Sugibayashi, Y. Shigeta

^*Corresponding author for this work

Clinical Pharmacology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

To elucidate the role of calmodulin in insulin action, we examined the effect of the calmodulin antagonists, W-7 and W-5, on glucose transport in isolated rat adipocytes. W-7 inhibited insulin-stimulated 2-deoxyglucose uptake by 18% at 100 μM, but it did not affect basal uptake levels. W-5, a less potent analogue of W-7, however, had no significant effect at the same concentration, indicating that the effect was specific to calmodulin. Similar results were observed in a 3-O-methylglucose uptake study. Kinetic analysis of 2-deoxyglucose uptake revealed that W-7 affected the insulin-induced increase in V_max but not K_m. These results suggest that calmodulin modifies insulin action in the glucose transport system.

Original language	English
Pages (from-to)	109-113
Number of pages	5
Journal	Diabetes Research and Clinical Practice
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/0168-8227(89)90114-9
State	Published - 1989/02/15

Keywords

Calmodulin
Hexose transport
Insulin
Insulin receptor
W-7

ASJC Scopus subject areas

Internal Medicine
Endocrinology, Diabetes and Metabolism
Endocrinology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0168-8227(89)90114-9

Cite this

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title = "W-7 specifically inhibits insulin-induced increase in glucose transport",

abstract = "To elucidate the role of calmodulin in insulin action, we examined the effect of the calmodulin antagonists, W-7 and W-5, on glucose transport in isolated rat adipocytes. W-7 inhibited insulin-stimulated 2-deoxyglucose uptake by 18% at 100 μM, but it did not affect basal uptake levels. W-5, a less potent analogue of W-7, however, had no significant effect at the same concentration, indicating that the effect was specific to calmodulin. Similar results were observed in a 3-O-methylglucose uptake study. Kinetic analysis of 2-deoxyglucose uptake revealed that W-7 affected the insulin-induced increase in Vmax but not Km. These results suggest that calmodulin modifies insulin action in the glucose transport system.",

keywords = "Calmodulin, Hexose transport, Insulin, Insulin receptor, W-7",

author = "O. Ishibashi and M. Kobayashi and T. Sasaoka and M. Sugibayashi and Y. Shigeta",

note = "Funding Information: * This study was supported in part by a research grant-in-aid from the Ministry of Education, Science and Culture, Japan.",

year = "1989",

month = feb,

day = "15",

doi = "10.1016/0168-8227(89)90114-9",

language = "英語",

volume = "6",

pages = "109--113",

journal = "Diabetes Research and Clinical Practice",

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TY - JOUR

T1 - W-7 specifically inhibits insulin-induced increase in glucose transport

AU - Ishibashi, O.

AU - Kobayashi, M.

AU - Sasaoka, T.

AU - Sugibayashi, M.

AU - Shigeta, Y.

N1 - Funding Information: * This study was supported in part by a research grant-in-aid from the Ministry of Education, Science and Culture, Japan.

PY - 1989/2/15

Y1 - 1989/2/15

N2 - To elucidate the role of calmodulin in insulin action, we examined the effect of the calmodulin antagonists, W-7 and W-5, on glucose transport in isolated rat adipocytes. W-7 inhibited insulin-stimulated 2-deoxyglucose uptake by 18% at 100 μM, but it did not affect basal uptake levels. W-5, a less potent analogue of W-7, however, had no significant effect at the same concentration, indicating that the effect was specific to calmodulin. Similar results were observed in a 3-O-methylglucose uptake study. Kinetic analysis of 2-deoxyglucose uptake revealed that W-7 affected the insulin-induced increase in Vmax but not Km. These results suggest that calmodulin modifies insulin action in the glucose transport system.

AB - To elucidate the role of calmodulin in insulin action, we examined the effect of the calmodulin antagonists, W-7 and W-5, on glucose transport in isolated rat adipocytes. W-7 inhibited insulin-stimulated 2-deoxyglucose uptake by 18% at 100 μM, but it did not affect basal uptake levels. W-5, a less potent analogue of W-7, however, had no significant effect at the same concentration, indicating that the effect was specific to calmodulin. Similar results were observed in a 3-O-methylglucose uptake study. Kinetic analysis of 2-deoxyglucose uptake revealed that W-7 affected the insulin-induced increase in Vmax but not Km. These results suggest that calmodulin modifies insulin action in the glucose transport system.

KW - Calmodulin

KW - Hexose transport

KW - Insulin

KW - Insulin receptor

KW - W-7

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U2 - 10.1016/0168-8227(89)90114-9

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M3 - 学術論文

C2 - 2647441

AN - SCOPUS:0024546416

SN - 0168-8227

VL - 6

SP - 109

EP - 113

JO - Diabetes Research and Clinical Practice

JF - Diabetes Research and Clinical Practice

IS - 2

ER -

W-7 specifically inhibits insulin-induced increase in glucose transport

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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