Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Hiroshi Mihara; Nobuhiro Suzuki; Ammar Abdullkader Boudaka; Jibran Sualeh Muhammad; Makoto Tominaga; Yoshiaki Tabuchi; Toshiro Sugiyama

doi:10.3748/wjg.v22.i24.5512

Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Hiroshi Mihara^*, Nobuhiro Suzuki, Ammar Abdullkader Boudaka, Jibran Sualeh Muhammad, Makoto Tominaga, Yoshiaki Tabuchi, Toshiro Sugiyama

^*Corresponding author for this work

Center for Medical Education and Career Development
Division of Molecular Genetics Research, Life Science Research Center, Organization for Promotion of Research

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.

Original language	English
Pages (from-to)	5512-5519
Number of pages	8
Journal	World Journal of Gastroenterology
Volume	22
Issue number	24
DOIs	https://doi.org/10.3748/wjg.v22.i24.5512
State	Published - 2016/06/28

Keywords

ATP
Gastric emptying
Stomach
Transient receptor potential vanilloid 4

ASJC Scopus subject areas

Gastroenterology

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10.3748/wjg.v22.i24.5512

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@article{571452043b6e4810b169a9254a500337,

title = "Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia",

abstract = "AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.",

keywords = "ATP, Gastric emptying, Stomach, Transient receptor potential vanilloid 4",

author = "Hiroshi Mihara and Nobuhiro Suzuki and Boudaka, {Ammar Abdullkader} and Muhammad, {Jibran Sualeh} and Makoto Tominaga and Yoshiaki Tabuchi and Toshiro Sugiyama",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2016.",

year = "2016",

month = jun,

day = "28",

doi = "10.3748/wjg.v22.i24.5512",

language = "英語",

volume = "22",

pages = "5512--5519",

journal = "World Journal of Gastroenterology",

issn = "1007-9327",

publisher = "Baishideng Publishing Group Inc",

number = "24",

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TY - JOUR

T1 - Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

AU - Mihara, Hiroshi

AU - Suzuki, Nobuhiro

AU - Boudaka, Ammar Abdullkader

AU - Muhammad, Jibran Sualeh

AU - Tominaga, Makoto

AU - Tabuchi, Yoshiaki

AU - Sugiyama, Toshiro

N1 - Publisher Copyright: © The Author(s) 2016.

PY - 2016/6/28

Y1 - 2016/6/28

N2 - AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.

AB - AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.

KW - ATP

KW - Gastric emptying

KW - Stomach

KW - Transient receptor potential vanilloid 4

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Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Abstract

Keywords

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