Titanium alloy modified with anti-biofouling zwitterionic polymer to facilitate formation of bio-mineral layer

Miku Nishida; Tadashi Nakaji-Hirabayashi; Hiromi Kitano; Yoshiyuki Saruwatari; Kazuyoshi Matsuoka

doi:10.1016/j.colsurfb.2017.01.018

Titanium alloy modified with anti-biofouling zwitterionic polymer to facilitate formation of bio-mineral layer

Miku Nishida, Tadashi Nakaji-Hirabayashi^*, Hiromi Kitano, Yoshiyuki Saruwatari, Kazuyoshi Matsuoka

^*Corresponding author for this work

Applied Chemistry

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The surface of a titanium (Ti) alloy was modified with a self-assembled monolayer of poly(ethylene glycol) methacrylate phosphate (Phosmer PE). A zwitterionic monomer (carboxymethyl betaine, CMB) could be copolymerized with the surface-bound Phosmer PE due to a flexible linker between the Ti alloy surface and a methacryloyl group of Phosmer PE. The poly(CMB) (PCMB)-modified Ti alloy plate exhibited strong suppression of protein adsorption and cell adhesion, and induced approximately twice the amount of calcium (Ca²⁺) deposition as compared to the unmodified Ti alloy plate. The zwitterionic polymer-modified surfaces not only showed enhanced mineralization clusters creation and growth, but they were also highly non-responsive to biologically derived materials such as proteins and cells. Therefore, it is possible to easily form highly pure and rigid hydroxyapatite layers on Ti alloy surfaces without the incorporation of organic molecules such as proteins. The present surface modification technique and strategy can be applied to implantable orthopedic materials as a means of encouraging integration with host tissues, such as the thigh bone.

Original language	English
Pages (from-to)	302-310
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	152
DOIs	https://doi.org/10.1016/j.colsurfb.2017.01.018
State	Published - 2017/04/01

Keywords

Calcium apatite
Flexible linker
Mineralization
Ti alloy plate
Zwitterionic polymer layer

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2017.01.018

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title = "Titanium alloy modified with anti-biofouling zwitterionic polymer to facilitate formation of bio-mineral layer",

abstract = "The surface of a titanium (Ti) alloy was modified with a self-assembled monolayer of poly(ethylene glycol) methacrylate phosphate (Phosmer PE). A zwitterionic monomer (carboxymethyl betaine, CMB) could be copolymerized with the surface-bound Phosmer PE due to a flexible linker between the Ti alloy surface and a methacryloyl group of Phosmer PE. The poly(CMB) (PCMB)-modified Ti alloy plate exhibited strong suppression of protein adsorption and cell adhesion, and induced approximately twice the amount of calcium (Ca2+) deposition as compared to the unmodified Ti alloy plate. The zwitterionic polymer-modified surfaces not only showed enhanced mineralization clusters creation and growth, but they were also highly non-responsive to biologically derived materials such as proteins and cells. Therefore, it is possible to easily form highly pure and rigid hydroxyapatite layers on Ti alloy surfaces without the incorporation of organic molecules such as proteins. The present surface modification technique and strategy can be applied to implantable orthopedic materials as a means of encouraging integration with host tissues, such as the thigh bone.",

keywords = "Calcium apatite, Flexible linker, Mineralization, Ti alloy plate, Zwitterionic polymer layer",

author = "Miku Nishida and Tadashi Nakaji-Hirabayashi and Hiromi Kitano and Yoshiyuki Saruwatari and Kazuyoshi Matsuoka",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

month = apr,

day = "1",

doi = "10.1016/j.colsurfb.2017.01.018",

language = "英語",

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T1 - Titanium alloy modified with anti-biofouling zwitterionic polymer to facilitate formation of bio-mineral layer

AU - Nishida, Miku

AU - Nakaji-Hirabayashi, Tadashi

AU - Kitano, Hiromi

AU - Saruwatari, Yoshiyuki

AU - Matsuoka, Kazuyoshi

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The surface of a titanium (Ti) alloy was modified with a self-assembled monolayer of poly(ethylene glycol) methacrylate phosphate (Phosmer PE). A zwitterionic monomer (carboxymethyl betaine, CMB) could be copolymerized with the surface-bound Phosmer PE due to a flexible linker between the Ti alloy surface and a methacryloyl group of Phosmer PE. The poly(CMB) (PCMB)-modified Ti alloy plate exhibited strong suppression of protein adsorption and cell adhesion, and induced approximately twice the amount of calcium (Ca2+) deposition as compared to the unmodified Ti alloy plate. The zwitterionic polymer-modified surfaces not only showed enhanced mineralization clusters creation and growth, but they were also highly non-responsive to biologically derived materials such as proteins and cells. Therefore, it is possible to easily form highly pure and rigid hydroxyapatite layers on Ti alloy surfaces without the incorporation of organic molecules such as proteins. The present surface modification technique and strategy can be applied to implantable orthopedic materials as a means of encouraging integration with host tissues, such as the thigh bone.

AB - The surface of a titanium (Ti) alloy was modified with a self-assembled monolayer of poly(ethylene glycol) methacrylate phosphate (Phosmer PE). A zwitterionic monomer (carboxymethyl betaine, CMB) could be copolymerized with the surface-bound Phosmer PE due to a flexible linker between the Ti alloy surface and a methacryloyl group of Phosmer PE. The poly(CMB) (PCMB)-modified Ti alloy plate exhibited strong suppression of protein adsorption and cell adhesion, and induced approximately twice the amount of calcium (Ca2+) deposition as compared to the unmodified Ti alloy plate. The zwitterionic polymer-modified surfaces not only showed enhanced mineralization clusters creation and growth, but they were also highly non-responsive to biologically derived materials such as proteins and cells. Therefore, it is possible to easily form highly pure and rigid hydroxyapatite layers on Ti alloy surfaces without the incorporation of organic molecules such as proteins. The present surface modification technique and strategy can be applied to implantable orthopedic materials as a means of encouraging integration with host tissues, such as the thigh bone.

KW - Calcium apatite

KW - Flexible linker

KW - Mineralization

KW - Ti alloy plate

KW - Zwitterionic polymer layer

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U2 - 10.1016/j.colsurfb.2017.01.018

DO - 10.1016/j.colsurfb.2017.01.018

M3 - 学術論文

C2 - 28129602

AN - SCOPUS:85010451184

SN - 0927-7765

VL - 152

SP - 302

EP - 310

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Titanium alloy modified with anti-biofouling zwitterionic polymer to facilitate formation of bio-mineral layer

Abstract

Keywords

ASJC Scopus subject areas

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