The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice

Noriko Nakanishi; Yoshimi Nakagawa; Naoko Tokushige; Naohito Aoki; Takashi Matsuzaka; Kiyoaki Ishii; Naoya Yahagi; Kazuto Kobayashi; Shigeru Yatoh; Akimitsu Takahashi; Hiroaki Suzuki; Osamu Urayama; Nobuhiro Yamada; Hitoshi Shimano

doi:10.1016/j.bbrc.2009.05.058

The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice

Noriko Nakanishi, Yoshimi Nakagawa^*, Naoko Tokushige, Naohito Aoki, Takashi Matsuzaka, Kiyoaki Ishii, Naoya Yahagi, Kazuto Kobayashi, Shigeru Yatoh, Akimitsu Takahashi, Hiroaki Suzuki, Osamu Urayama, Nobuhiro Yamada, Hitoshi Shimano

^*Corresponding author for this work

Complex Biosystem Research, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

168 Scopus citations

Abstract

MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. Some miRNAs have been proposed to be associated with obesity. However, miRNAs, which are related to the development of obesity in vivo remains unknown. Here in, we found the up-regulation of miR-335 in obesity using microarray analysis for miRNA. The expressions of miR-335 in liver and white adipose tissue (WAT) were up-regulated in obese mice including ob/ob, db/db, and KKAy mice. Increased miR-335 expressions were associated with an elevated body, liver and WAT weight, and hepatic triglyceride and cholesterol. Furthermore, miR-335 levels were closely correlated with expression levels of adipocyte differentiation markers such as PPARγ, aP2, and FAS in 3T3-L1 adipocyte. These findings provide the first evidence that the up-regulated expressions of miR-335 in liver and WAT of obese mice might contribute to the pathophysiology of obesity.

Original language	English
Pages (from-to)	492-496
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	385
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.05.058
State	Published - 2009/08/07

Keywords

Adipogenesis
Diabetes
Lipid metabolism
MicroRNA
Obesity

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2009.05.058

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Nakanishi, N., Nakagawa, Y., Tokushige, N., Aoki, N., Matsuzaka, T., Ishii, K., Yahagi, N., Kobayashi, K., Yatoh, S., Takahashi, A., Suzuki, H., Urayama, O., Yamada, N., & Shimano, H. (2009). The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice. Biochemical and Biophysical Research Communications, 385(4), 492-496. https://doi.org/10.1016/j.bbrc.2009.05.058

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title = "The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice",

abstract = "MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. Some miRNAs have been proposed to be associated with obesity. However, miRNAs, which are related to the development of obesity in vivo remains unknown. Here in, we found the up-regulation of miR-335 in obesity using microarray analysis for miRNA. The expressions of miR-335 in liver and white adipose tissue (WAT) were up-regulated in obese mice including ob/ob, db/db, and KKAy mice. Increased miR-335 expressions were associated with an elevated body, liver and WAT weight, and hepatic triglyceride and cholesterol. Furthermore, miR-335 levels were closely correlated with expression levels of adipocyte differentiation markers such as PPARγ, aP2, and FAS in 3T3-L1 adipocyte. These findings provide the first evidence that the up-regulated expressions of miR-335 in liver and WAT of obese mice might contribute to the pathophysiology of obesity.",

keywords = "Adipogenesis, Diabetes, Lipid metabolism, MicroRNA, Obesity",

author = "Noriko Nakanishi and Yoshimi Nakagawa and Naoko Tokushige and Naohito Aoki and Takashi Matsuzaka and Kiyoaki Ishii and Naoya Yahagi and Kazuto Kobayashi and Shigeru Yatoh and Akimitsu Takahashi and Hiroaki Suzuki and Osamu Urayama and Nobuhiro Yamada and Hitoshi Shimano",

note = "Funding Information: This work was supported by Grants-in-Aid from the Ministry of Science, Education, Culture, and Technology of Japan (Y.N., N.A., T.M., A.T., S.Y., H.S., K.K., K.I., and H.S.). ",

year = "2009",

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doi = "10.1016/j.bbrc.2009.05.058",

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Nakanishi, N, Nakagawa, Y, Tokushige, N, Aoki, N, Matsuzaka, T, Ishii, K, Yahagi, N, Kobayashi, K, Yatoh, S, Takahashi, A, Suzuki, H, Urayama, O, Yamada, N & Shimano, H 2009, 'The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice', Biochemical and Biophysical Research Communications, vol. 385, no. 4, pp. 492-496. https://doi.org/10.1016/j.bbrc.2009.05.058

TY - JOUR

T1 - The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice

AU - Nakanishi, Noriko

AU - Nakagawa, Yoshimi

AU - Tokushige, Naoko

AU - Aoki, Naohito

AU - Matsuzaka, Takashi

AU - Ishii, Kiyoaki

AU - Yahagi, Naoya

AU - Kobayashi, Kazuto

AU - Yatoh, Shigeru

AU - Takahashi, Akimitsu

AU - Suzuki, Hiroaki

AU - Urayama, Osamu

AU - Yamada, Nobuhiro

AU - Shimano, Hitoshi

N1 - Funding Information: This work was supported by Grants-in-Aid from the Ministry of Science, Education, Culture, and Technology of Japan (Y.N., N.A., T.M., A.T., S.Y., H.S., K.K., K.I., and H.S.).

PY - 2009/8/7

Y1 - 2009/8/7

N2 - MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. Some miRNAs have been proposed to be associated with obesity. However, miRNAs, which are related to the development of obesity in vivo remains unknown. Here in, we found the up-regulation of miR-335 in obesity using microarray analysis for miRNA. The expressions of miR-335 in liver and white adipose tissue (WAT) were up-regulated in obese mice including ob/ob, db/db, and KKAy mice. Increased miR-335 expressions were associated with an elevated body, liver and WAT weight, and hepatic triglyceride and cholesterol. Furthermore, miR-335 levels were closely correlated with expression levels of adipocyte differentiation markers such as PPARγ, aP2, and FAS in 3T3-L1 adipocyte. These findings provide the first evidence that the up-regulated expressions of miR-335 in liver and WAT of obese mice might contribute to the pathophysiology of obesity.

AB - MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. Some miRNAs have been proposed to be associated with obesity. However, miRNAs, which are related to the development of obesity in vivo remains unknown. Here in, we found the up-regulation of miR-335 in obesity using microarray analysis for miRNA. The expressions of miR-335 in liver and white adipose tissue (WAT) were up-regulated in obese mice including ob/ob, db/db, and KKAy mice. Increased miR-335 expressions were associated with an elevated body, liver and WAT weight, and hepatic triglyceride and cholesterol. Furthermore, miR-335 levels were closely correlated with expression levels of adipocyte differentiation markers such as PPARγ, aP2, and FAS in 3T3-L1 adipocyte. These findings provide the first evidence that the up-regulated expressions of miR-335 in liver and WAT of obese mice might contribute to the pathophysiology of obesity.

KW - Adipogenesis

KW - Diabetes

KW - Lipid metabolism

KW - MicroRNA

KW - Obesity

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U2 - 10.1016/j.bbrc.2009.05.058

DO - 10.1016/j.bbrc.2009.05.058

M3 - 学術論文

C2 - 19460359

AN - SCOPUS:67349179168

SN - 0006-291X

VL - 385

SP - 492

EP - 496

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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