TY - JOUR
T1 - The rps4 gene in sugar beet mitochondria
T2 - Insertion/deletion mutations occur within the gene but do not disrupt the reading frame
AU - Itchoda, Noriko
AU - Kubo, Tomohiko
AU - Estiati, Amy
AU - Yamamoto, Masayuki P.
AU - Handa, Hirokazu
AU - Mikami, Tetsuo
N1 - Funding Information:
Acknowledgements. This work was done in part at the Research Center for Molecular Genetics, Hokkaido University, and was supported in part by Grants in Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan and a Grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences, Japan. A. E. is the recipient of a Japanese Government (Monbusho) Scholarship. M. P. Y. is the recipient of a JSPS Fellowship for Japanese Junior Scientists.
PY - 2002
Y1 - 2002
N2 - We have characterized a sugar beet mitochondrial gene, rps4, which is co-transcribed with the downstream gene nad6. The sugar beet RPS4 polypeptide has two deletions of 8 and 49 amino acid residues and a duplication of 14 residues in the middle of the reading frame, relative to the rapeseed and rice homologues. Nevertheless, transcripts of the rps4 were observed to be edited at 10 nucleotides, suggesting that the sugar beet rps4 is functional. Western blot analysis identified the translation product of 33kDa. The extensive rearrangements in rps4 were also found in spinach but not in pea, green pepper and rose.
AB - We have characterized a sugar beet mitochondrial gene, rps4, which is co-transcribed with the downstream gene nad6. The sugar beet RPS4 polypeptide has two deletions of 8 and 49 amino acid residues and a duplication of 14 residues in the middle of the reading frame, relative to the rapeseed and rice homologues. Nevertheless, transcripts of the rps4 were observed to be edited at 10 nucleotides, suggesting that the sugar beet rps4 is functional. Western blot analysis identified the translation product of 33kDa. The extensive rearrangements in rps4 were also found in spinach but not in pea, green pepper and rose.
KW - Gene expression
KW - Insertions/deletions
KW - Mitochondria
KW - Rps4
KW - Sugar beet
UR - http://www.scopus.com/inward/record.url?scp=0036195612&partnerID=8YFLogxK
U2 - 10.1078/0176-1617-00687
DO - 10.1078/0176-1617-00687
M3 - 学術論文
AN - SCOPUS:0036195612
SN - 0176-1617
VL - 159
SP - 211
EP - 215
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 2
ER -