The chloroplast gene for ribosomal protein CL23 is functional in tobacco

Fumiaki Yokoi; Minoru Tanaka; Tatsuya Wakasugi; Masahiro Sugiura

doi:10.1016/0014-5793(91)80359-B

The chloroplast gene for ribosomal protein CL23 is functional in tobacco

Fumiaki Yokoi, Minoru Tanaka, Tatsuya Wakasugi, Masahiro Sugiura^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Chloroplast rp123 loci potentially coding for a polypeptide homologous to the E. Coli L23 ribosomal protein are frame-shifted in spinach and several other plants, indicating that these loci are pseudogenes. In tobacco, tp123 constitutes a continuous open reading frame of 93 codons and its transcript initiates at least 66 bp upstream from the initiation codon. The N-terminal amino acid sequence of a 13 kDa protein from the 50 S subunit of tobacco chloroplast ribosomes matches that derived from the tobacco rp123 locus. This shows that rp123 is a functional gene in tobacco.

Original language	English
Pages (from-to)	64-66
Number of pages	3
Journal	FEBS Letters
Volume	281
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80359-B
State	Published - 1991/04/09

Keywords

Chloroplast
Pseudogene
Ribosomal protein
Tobacco
rp123

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80359-B

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title = "The chloroplast gene for ribosomal protein CL23 is functional in tobacco",

abstract = "Chloroplast rp123 loci potentially coding for a polypeptide homologous to the E. Coli L23 ribosomal protein are frame-shifted in spinach and several other plants, indicating that these loci are pseudogenes. In tobacco, tp123 constitutes a continuous open reading frame of 93 codons and its transcript initiates at least 66 bp upstream from the initiation codon. The N-terminal amino acid sequence of a 13 kDa protein from the 50 S subunit of tobacco chloroplast ribosomes matches that derived from the tobacco rp123 locus. This shows that rp123 is a functional gene in tobacco.",

keywords = "Chloroplast, Pseudogene, Ribosomal protein, Tobacco, rp123",

author = "Fumiaki Yokoi and Minoru Tanaka and Tatsuya Wakasugi and Masahiro Sugiura",

note = "Funding Information: Acknowledgemert/.c We thank Dr. A. Vassileva and Dr, N. Hayashida for help, and Dr. M. Sugita and Mr. K. Torazawa for sug. gestions and discussions. This work wns supported by a Grant-in-Aid from the Ministry of Education.",

year = "1991",

month = apr,

day = "9",

doi = "10.1016/0014-5793(91)80359-B",

language = "英語",

volume = "281",

pages = "64--66",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1-2",

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TY - JOUR

T1 - The chloroplast gene for ribosomal protein CL23 is functional in tobacco

AU - Yokoi, Fumiaki

AU - Tanaka, Minoru

AU - Wakasugi, Tatsuya

AU - Sugiura, Masahiro

N1 - Funding Information: Acknowledgemert/.c We thank Dr. A. Vassileva and Dr, N. Hayashida for help, and Dr. M. Sugita and Mr. K. Torazawa for sug. gestions and discussions. This work wns supported by a Grant-in-Aid from the Ministry of Education.

PY - 1991/4/9

Y1 - 1991/4/9

N2 - Chloroplast rp123 loci potentially coding for a polypeptide homologous to the E. Coli L23 ribosomal protein are frame-shifted in spinach and several other plants, indicating that these loci are pseudogenes. In tobacco, tp123 constitutes a continuous open reading frame of 93 codons and its transcript initiates at least 66 bp upstream from the initiation codon. The N-terminal amino acid sequence of a 13 kDa protein from the 50 S subunit of tobacco chloroplast ribosomes matches that derived from the tobacco rp123 locus. This shows that rp123 is a functional gene in tobacco.

AB - Chloroplast rp123 loci potentially coding for a polypeptide homologous to the E. Coli L23 ribosomal protein are frame-shifted in spinach and several other plants, indicating that these loci are pseudogenes. In tobacco, tp123 constitutes a continuous open reading frame of 93 codons and its transcript initiates at least 66 bp upstream from the initiation codon. The N-terminal amino acid sequence of a 13 kDa protein from the 50 S subunit of tobacco chloroplast ribosomes matches that derived from the tobacco rp123 locus. This shows that rp123 is a functional gene in tobacco.

KW - Chloroplast

KW - Pseudogene

KW - Ribosomal protein

KW - Tobacco

KW - rp123

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U2 - 10.1016/0014-5793(91)80359-B

DO - 10.1016/0014-5793(91)80359-B

M3 - 学術論文

C2 - 1707833

AN - SCOPUS:0026029208

SN - 0014-5793

VL - 281

SP - 64

EP - 66

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

The chloroplast gene for ribosomal protein CL23 is functional in tobacco

Abstract

Keywords

ASJC Scopus subject areas

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