The anorexigenic effect of cholecystokinin octapeptide in a goldfish model is mediated by the vagal afferent and subsequently through the melanocortin- and corticotropin-releasing hormone-signaling pathways

Ki Sung Kang, Satowa Yahashi, Morio Azuma, Kouhei Matsuda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

We have been extensively investigating the mechanisms by which neuropeptides regulate feeding behavior by using a goldfish (Carassius auratus) model. In this species, the anorexigenic action of melanocortin peptide is centrally mediated via the corticotropin-releasing hormone (CRH)/CRH receptor neuronal system, whereas sulfated cholecystokinin octapeptide (CCK-8s) is involved in the appetite regulation as a peripheral anorexigenic factor. The aim of the present study was to identify the mechanism of the anorexigenic effect of peripherally injected CCK-8s, which has not yet been identified in goldfish. Co-administration of capsaicin, a neurotoxin that destroys primary sensory afferents, at 100 nmol/g BW, blocked the anorexigenic action of intraperitoneally injected CCK-8s (100 pmol/g BW), whereas the anorexigenic action of intracerebroventricularly injected CCK-8s (5 pmol/g BW) was not blocked by co-administration of capsaicin. Pre-treatment with a specific CRH receptor antagonist, α-helical CRH(9-41), attenuated the anorexigenic action of CCK-8s. The expression level of CRH mRNA in the diencephalic tissue of the CCK-8s-injected group was not changed, but the level of proopiomelanocortin mRNA was significantly increased at 1 h after treatment. Therefore, we have identified for the first time that the reduction of appetite induced by peripherally injected CCK-8s in goldfish appears to be mediated by the vagal afferent and subsequently through the melanocortin- and corticotropin-releasing hormone-signaling pathways.

Original languageEnglish
Pages (from-to)2130-2134
Number of pages5
JournalPeptides
Volume31
Issue number11
DOIs
StatePublished - 2010/11

Keywords

  • Anorexigenic action
  • Corticotropin-releasing hormone
  • Feeding behavior
  • Goldfish
  • Proopiomelanocortin
  • Sulfated cholecystokinin octapeptide
  • Vagal afferent

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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