Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells

Natsuko Kageyama-Yahara; Xiaoyu Wang; Tatsuo Katagiri; Ping Wang; Takeshi Yamamoto; Makoto Tominaga; Makoto Kadowaki

doi:10.1016/j.bbrc.2011.11.014

Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells

Natsuko Kageyama-Yahara, Xiaoyu Wang, Tatsuo Katagiri, Ping Wang, Takeshi Yamamoto, Makoto Tominaga, Makoto Kadowaki^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Antigen-IgE-mediated mucosal mast-cell activation is critical in the development of food allergies. Cinnamaldehyde, a major constituent of Cinnamomi cortex, dose-dependently inhibited the antigen-IgE-induced degranulation of mucosal-type bone-marrow derived mast cells (mBMMCs) and RBL-2H3 cells. Cinnamaldehyde also suppressed the elevation of the intracellular Ca ²⁺ level that is induced by the extracellular Ca ²⁺ influx in antigen-IgE-stimulated mBMMCs. Furthermore, tyrosine phosphorylation of phospholipase C (PLC) γ1, which is a crucial activation switch for the intracellular Ca ²⁺ mobilization in mast cells, was attenuated by cinnamaldehyde. Together, our results demonstrated that cinnamaldehyde suppressed the intracellular Ca ²⁺ mobilization and the degranulation of mucosal mast cells by inhibiting the activity of the IgE receptor-PLCγ-Ca ²⁺ influx pathway. These findings suggest that cinnamaldehyde may have therapeutic potential in mucosal mast cell-related allergic diseases, such as food allergies.

Original language	English
Pages (from-to)	283-288
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	416
Issue number	3-4
DOIs	https://doi.org/10.1016/j.bbrc.2011.11.014
State	Published - 2011/12/16

Keywords

Ca influx
Ca mobilization
Cinnamaldehyde
Mucosal mast cells
Phospholipase Cγ1
TRPA1

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2011.11.014

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Kageyama-Yahara, N., Wang, X., Katagiri, T., Wang, P., Yamamoto, T., Tominaga, M., & Kadowaki, M. (2011). Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells. Biochemical and Biophysical Research Communications, 416(3-4), 283-288. https://doi.org/10.1016/j.bbrc.2011.11.014

@article{3241155e91b6414fb790e9a7ce7ed5e4,

title = "Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells",

abstract = "Antigen-IgE-mediated mucosal mast-cell activation is critical in the development of food allergies. Cinnamaldehyde, a major constituent of Cinnamomi cortex, dose-dependently inhibited the antigen-IgE-induced degranulation of mucosal-type bone-marrow derived mast cells (mBMMCs) and RBL-2H3 cells. Cinnamaldehyde also suppressed the elevation of the intracellular Ca 2+ level that is induced by the extracellular Ca 2+ influx in antigen-IgE-stimulated mBMMCs. Furthermore, tyrosine phosphorylation of phospholipase C (PLC) γ1, which is a crucial activation switch for the intracellular Ca 2+ mobilization in mast cells, was attenuated by cinnamaldehyde. Together, our results demonstrated that cinnamaldehyde suppressed the intracellular Ca 2+ mobilization and the degranulation of mucosal mast cells by inhibiting the activity of the IgE receptor-PLCγ-Ca 2+ influx pathway. These findings suggest that cinnamaldehyde may have therapeutic potential in mucosal mast cell-related allergic diseases, such as food allergies.",

keywords = "Ca influx, Ca mobilization, Cinnamaldehyde, Mucosal mast cells, Phospholipase Cγ1, TRPA1",

author = "Natsuko Kageyama-Yahara and Xiaoyu Wang and Tatsuo Katagiri and Ping Wang and Takeshi Yamamoto and Makoto Tominaga and Makoto Kadowaki",

note = "Funding Information: This research was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to T. Yamamoto (No. 20790472) and M. Kadowaki (No. 21590760), the Knowledge Cluster Initiative Program (Second Stage) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to M. Kadowaki, and a Grant-in-Aid for the Cooperative Research Project from Joint Usage/Research Center for Science-Based Natural Medicine, Institute of Natural Medicine, University of Toyama in 2009-2011.",

year = "2011",

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doi = "10.1016/j.bbrc.2011.11.014",

language = "英語",

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Kageyama-Yahara, N, Wang, X, Katagiri, T, Wang, P, Yamamoto, T, Tominaga, M & Kadowaki, M 2011, 'Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells', Biochemical and Biophysical Research Communications, vol. 416, no. 3-4, pp. 283-288. https://doi.org/10.1016/j.bbrc.2011.11.014

Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells. / Kageyama-Yahara, Natsuko; Wang, Xiaoyu; Katagiri, Tatsuo et al.
In: Biochemical and Biophysical Research Communications, Vol. 416, No. 3-4, 16.12.2011, p. 283-288.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells

AU - Kageyama-Yahara, Natsuko

AU - Wang, Xiaoyu

AU - Katagiri, Tatsuo

AU - Wang, Ping

AU - Yamamoto, Takeshi

AU - Tominaga, Makoto

AU - Kadowaki, Makoto

N1 - Funding Information: This research was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to T. Yamamoto (No. 20790472) and M. Kadowaki (No. 21590760), the Knowledge Cluster Initiative Program (Second Stage) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to M. Kadowaki, and a Grant-in-Aid for the Cooperative Research Project from Joint Usage/Research Center for Science-Based Natural Medicine, Institute of Natural Medicine, University of Toyama in 2009-2011.

PY - 2011/12/16

Y1 - 2011/12/16

N2 - Antigen-IgE-mediated mucosal mast-cell activation is critical in the development of food allergies. Cinnamaldehyde, a major constituent of Cinnamomi cortex, dose-dependently inhibited the antigen-IgE-induced degranulation of mucosal-type bone-marrow derived mast cells (mBMMCs) and RBL-2H3 cells. Cinnamaldehyde also suppressed the elevation of the intracellular Ca 2+ level that is induced by the extracellular Ca 2+ influx in antigen-IgE-stimulated mBMMCs. Furthermore, tyrosine phosphorylation of phospholipase C (PLC) γ1, which is a crucial activation switch for the intracellular Ca 2+ mobilization in mast cells, was attenuated by cinnamaldehyde. Together, our results demonstrated that cinnamaldehyde suppressed the intracellular Ca 2+ mobilization and the degranulation of mucosal mast cells by inhibiting the activity of the IgE receptor-PLCγ-Ca 2+ influx pathway. These findings suggest that cinnamaldehyde may have therapeutic potential in mucosal mast cell-related allergic diseases, such as food allergies.

AB - Antigen-IgE-mediated mucosal mast-cell activation is critical in the development of food allergies. Cinnamaldehyde, a major constituent of Cinnamomi cortex, dose-dependently inhibited the antigen-IgE-induced degranulation of mucosal-type bone-marrow derived mast cells (mBMMCs) and RBL-2H3 cells. Cinnamaldehyde also suppressed the elevation of the intracellular Ca 2+ level that is induced by the extracellular Ca 2+ influx in antigen-IgE-stimulated mBMMCs. Furthermore, tyrosine phosphorylation of phospholipase C (PLC) γ1, which is a crucial activation switch for the intracellular Ca 2+ mobilization in mast cells, was attenuated by cinnamaldehyde. Together, our results demonstrated that cinnamaldehyde suppressed the intracellular Ca 2+ mobilization and the degranulation of mucosal mast cells by inhibiting the activity of the IgE receptor-PLCγ-Ca 2+ influx pathway. These findings suggest that cinnamaldehyde may have therapeutic potential in mucosal mast cell-related allergic diseases, such as food allergies.

KW - Ca influx

KW - Ca mobilization

KW - Cinnamaldehyde

KW - Mucosal mast cells

KW - Phospholipase Cγ1

KW - TRPA1

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U2 - 10.1016/j.bbrc.2011.11.014

DO - 10.1016/j.bbrc.2011.11.014

M3 - 学術論文

C2 - 22100653

AN - SCOPUS:84855886278

SN - 0006-291X

VL - 416

SP - 283

EP - 288

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3-4

ER -

Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells

Abstract

Keywords

ASJC Scopus subject areas

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