Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages

Hirohito Sone; Hitoshi Shimano; Miao Shu; Masanori Nakakuki; Akimitsu Takahashi; Masakazu Sakai; Yuichiro Sakamoto; Tomotaka Yokoo; Ken Matsuzaka; Hiroaki Okazaki; Yoshimi Nakagawa; Kaoruko Tada Iida; Hiroaki Suzuki; Hideo Toyoshima; Seikoh Horiuchi; Nobuhiro Yamada

doi:10.1016/j.bbrc.2004.02.121

Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages

Hirohito Sone, Hitoshi Shimano^*, Miao Shu, Masanori Nakakuki, Akimitsu Takahashi, Masakazu Sakai, Yuichiro Sakamoto, Tomotaka Yokoo, Ken Matsuzaka, Hiroaki Okazaki, Yoshimi Nakagawa, Kaoruko Tada Iida, Hiroaki Suzuki, Hideo Toyoshima, Seikoh Horiuchi, Nobuhiro Yamada

^*Corresponding author for this work

Complex Biosystem Research, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The ATP-binding-cassette transporter A1 (ABCA1) plays an essential role in cellular cholesterol efflux and helps prevent macrophages from becoming foam cells. The statins are widely used as cholesterol-lowering agents and have other anti-atherogenic actions. We tested the effects of four different statins (fluvastatin, atorvastatin, simvastatin, and lovastatin) on ABCA1 expression in macrophages in vitro. The statins suppressed ABCA1 mRNA expression in RAW246.7 and THP-1 macrophage cell lines and in mouse peritoneal macrophages. The effect was time- and dose-dependent and was abolished by the addition of the post-reductase product, mevalonate. These findings imply that there is a possible modulation of the well-known beneficial effects of the statins on the reverse cholesterol transport pathway.

Original language	English
Pages (from-to)	790-794
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	316
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.02.121
State	Published - 2004/04/09

Keywords

ATP-binding-cassette transporter A1
Macrophage
Reverse cholesterol transport
Statins (HMG-CoA reductase inhibitors)

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.02.121

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Sone, H., Shimano, H., Shu, M., Nakakuki, M., Takahashi, A., Sakai, M., Sakamoto, Y., Yokoo, T., Matsuzaka, K., Okazaki, H., Nakagawa, Y., Iida, K. T., Suzuki, H., Toyoshima, H., Horiuchi, S., & Yamada, N. (2004). Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages. Biochemical and Biophysical Research Communications, 316(3), 790-794. https://doi.org/10.1016/j.bbrc.2004.02.121

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title = "Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages",

abstract = "The ATP-binding-cassette transporter A1 (ABCA1) plays an essential role in cellular cholesterol efflux and helps prevent macrophages from becoming foam cells. The statins are widely used as cholesterol-lowering agents and have other anti-atherogenic actions. We tested the effects of four different statins (fluvastatin, atorvastatin, simvastatin, and lovastatin) on ABCA1 expression in macrophages in vitro. The statins suppressed ABCA1 mRNA expression in RAW246.7 and THP-1 macrophage cell lines and in mouse peritoneal macrophages. The effect was time- and dose-dependent and was abolished by the addition of the post-reductase product, mevalonate. These findings imply that there is a possible modulation of the well-known beneficial effects of the statins on the reverse cholesterol transport pathway.",

keywords = "ATP-binding-cassette transporter A1, Macrophage, Reverse cholesterol transport, Statins (HMG-CoA reductase inhibitors)",

author = "Hirohito Sone and Hitoshi Shimano and Miao Shu and Masanori Nakakuki and Akimitsu Takahashi and Masakazu Sakai and Yuichiro Sakamoto and Tomotaka Yokoo and Ken Matsuzaka and Hiroaki Okazaki and Yoshimi Nakagawa and Iida, {Kaoruko Tada} and Hiroaki Suzuki and Hideo Toyoshima and Seikoh Horiuchi and Nobuhiro Yamada",

note = "Funding Information: The authors thank Ms. Reiko Takada, BSc, Mr. Noriyuki Inoue, MSc, Ms. Magali Berthoze, and Ms. Chizuko Fukui in University of Tsukuba for expert technical assistance. This work is supported by Research Project Grant of University of Tsukuba. H.S. is a recipient of a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (#12770632/14571084), and of Research Grants from the Uehara Memorial, and Meiji Kosei, Foundations.",

year = "2004",

month = apr,

day = "9",

doi = "10.1016/j.bbrc.2004.02.121",

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Sone, H, Shimano, H, Shu, M, Nakakuki, M, Takahashi, A, Sakai, M, Sakamoto, Y, Yokoo, T, Matsuzaka, K, Okazaki, H, Nakagawa, Y, Iida, KT, Suzuki, H, Toyoshima, H, Horiuchi, S & Yamada, N 2004, 'Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages', Biochemical and Biophysical Research Communications, vol. 316, no. 3, pp. 790-794. https://doi.org/10.1016/j.bbrc.2004.02.121

TY - JOUR

T1 - Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages

AU - Sone, Hirohito

AU - Shimano, Hitoshi

AU - Shu, Miao

AU - Nakakuki, Masanori

AU - Takahashi, Akimitsu

AU - Sakai, Masakazu

AU - Sakamoto, Yuichiro

AU - Yokoo, Tomotaka

AU - Matsuzaka, Ken

AU - Okazaki, Hiroaki

AU - Nakagawa, Yoshimi

AU - Iida, Kaoruko Tada

AU - Suzuki, Hiroaki

AU - Toyoshima, Hideo

AU - Horiuchi, Seikoh

AU - Yamada, Nobuhiro

N1 - Funding Information: The authors thank Ms. Reiko Takada, BSc, Mr. Noriyuki Inoue, MSc, Ms. Magali Berthoze, and Ms. Chizuko Fukui in University of Tsukuba for expert technical assistance. This work is supported by Research Project Grant of University of Tsukuba. H.S. is a recipient of a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (#12770632/14571084), and of Research Grants from the Uehara Memorial, and Meiji Kosei, Foundations.

PY - 2004/4/9

Y1 - 2004/4/9

N2 - The ATP-binding-cassette transporter A1 (ABCA1) plays an essential role in cellular cholesterol efflux and helps prevent macrophages from becoming foam cells. The statins are widely used as cholesterol-lowering agents and have other anti-atherogenic actions. We tested the effects of four different statins (fluvastatin, atorvastatin, simvastatin, and lovastatin) on ABCA1 expression in macrophages in vitro. The statins suppressed ABCA1 mRNA expression in RAW246.7 and THP-1 macrophage cell lines and in mouse peritoneal macrophages. The effect was time- and dose-dependent and was abolished by the addition of the post-reductase product, mevalonate. These findings imply that there is a possible modulation of the well-known beneficial effects of the statins on the reverse cholesterol transport pathway.

AB - The ATP-binding-cassette transporter A1 (ABCA1) plays an essential role in cellular cholesterol efflux and helps prevent macrophages from becoming foam cells. The statins are widely used as cholesterol-lowering agents and have other anti-atherogenic actions. We tested the effects of four different statins (fluvastatin, atorvastatin, simvastatin, and lovastatin) on ABCA1 expression in macrophages in vitro. The statins suppressed ABCA1 mRNA expression in RAW246.7 and THP-1 macrophage cell lines and in mouse peritoneal macrophages. The effect was time- and dose-dependent and was abolished by the addition of the post-reductase product, mevalonate. These findings imply that there is a possible modulation of the well-known beneficial effects of the statins on the reverse cholesterol transport pathway.

KW - ATP-binding-cassette transporter A1

KW - Macrophage

KW - Reverse cholesterol transport

KW - Statins (HMG-CoA reductase inhibitors)

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U2 - 10.1016/j.bbrc.2004.02.121

DO - 10.1016/j.bbrc.2004.02.121

M3 - 学術論文

C2 - 15033469

AN - SCOPUS:12144291619

SN - 0006-291X

VL - 316

SP - 790

EP - 794

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages

Abstract

Keywords

ASJC Scopus subject areas

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