TY - JOUR
T1 - Simultaneous determination of triterpene saponins in ginseng drugs by high-performance liquid chromatography
AU - Zhu, Shu
AU - Zou, Kun
AU - Cai, Shaoqing
AU - Meselhy, Meselhy Ragab
AU - Komatsu, Katsuko
PY - 2004/8
Y1 - 2004/8
N2 - A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mM K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signal/noise≥3) were 0.1 μg for ginsenosides Rb1, Rc, Rd, Re and Rg1, chikusetsusaponin III, and notoginsenoside R2, 0.2 μg for gisenoside Ro and chikusetsusaponin IVa, 0.3 μg for chikusetsusaponin IV, and 3 μg for majonoside R2. The calibration curve of each saponin had a correlation coefficient close to 1. Intraand interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved.
AB - A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mM K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signal/noise≥3) were 0.1 μg for ginsenosides Rb1, Rc, Rd, Re and Rg1, chikusetsusaponin III, and notoginsenoside R2, 0.2 μg for gisenoside Ro and chikusetsusaponin IVa, 0.3 μg for chikusetsusaponin IV, and 3 μg for majonoside R2. The calibration curve of each saponin had a correlation coefficient close to 1. Intraand interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved.
KW - Ginseng drug
KW - Ginsenoside
KW - HPLC method
KW - Panax spp
KW - Quantitative comparison
UR - http://www.scopus.com/inward/record.url?scp=14944354081&partnerID=8YFLogxK
U2 - 10.1248/cpb.52.995
DO - 10.1248/cpb.52.995
M3 - 学術論文
C2 - 15305000
AN - SCOPUS:14944354081
SN - 0009-2363
VL - 52
SP - 995
EP - 998
JO - Chemical and Pharmaceutical Bulletin
JF - Chemical and Pharmaceutical Bulletin
IS - 8
ER -