Short-term and bystander effects of radiation on murine submandibular glands

Hitoshi Uchida; Matthew H. Ingalls; Eri O. Maruyama; Carl J. Johnston; Eric Hernady; Roberta C. Faustoferri; Catherine E. Ovitt

doi:10.1242/dmm.049570

Short-term and bystander effects of radiation on murine submandibular glands

Hitoshi Uchida, Matthew H. Ingalls, Eri O. Maruyama, Carl J. Johnston, Eric Hernady, Roberta C. Faustoferri, Catherine E. Ovitt^*

^*Corresponding author for this work

Molecular and Medical Pharmacology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Many patients treated for head and neck cancers experience salivary gland hypofunction due to radiation damage. Understanding the mechanisms of cellular damage induced by radiation treatment is important in order to design methods of radioprotection. In addition, it is crucial to recognize the indirect effects of IR and the systemic responses that may alter saliva secretion. In this study, radiation was delivered to murine submandibular glands (SMG) bilaterally, using a ¹³⁷Cs gamma ray irradiator, or unilaterally, using a small animal radiation research platform (SARRP). Analysis at 3, 24 and 48 hours showed dynamic changes in mRNA levels and protein abundance in SMG irradiated bilaterally. Unilateral irradiation using the SARRP caused similar changes in the irradiated SMG, as well as significant off-target, bystander effects in the non-irradiated contralateral SMG.

Original language	English
Journal	DMM Disease Models and Mechanisms
Volume	15
Issue number	11
DOIs	https://doi.org/10.1242/dmm.049570
State	Published - 2022/11

Keywords

Salivary glands
bystander effects
radiation
small animal radiation research platform

ASJC Scopus subject areas

Neuroscience (miscellaneous)
Medicine (miscellaneous)
Immunology and Microbiology (miscellaneous)
General Biochemistry, Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1242/dmm.049570

Cite this

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title = "Short-term and bystander effects of radiation on murine submandibular glands",

abstract = "Many patients treated for head and neck cancers experience salivary gland hypofunction due to radiation damage. Understanding the mechanisms of cellular damage induced by radiation treatment is important in order to design methods of radioprotection. In addition, it is crucial to recognize the indirect effects of IR and the systemic responses that may alter saliva secretion. In this study, radiation was delivered to murine submandibular glands (SMG) bilaterally, using a 137Cs gamma ray irradiator, or unilaterally, using a small animal radiation research platform (SARRP). Analysis at 3, 24 and 48 hours showed dynamic changes in mRNA levels and protein abundance in SMG irradiated bilaterally. Unilateral irradiation using the SARRP caused similar changes in the irradiated SMG, as well as significant off-target, bystander effects in the non-irradiated contralateral SMG.",

keywords = "Salivary glands, bystander effects, radiation, small animal radiation research platform",

author = "Hitoshi Uchida and Ingalls, {Matthew H.} and Maruyama, {Eri O.} and Johnston, {Carl J.} and Eric Hernady and Faustoferri, {Roberta C.} and Ovitt, {Catherine E.}",

note = "Publisher Copyright: {\textcopyright} 2022. Published by The Company of Biologists Ltd.",

year = "2022",

month = nov,

doi = "10.1242/dmm.049570",

language = "英語",

volume = "15",

journal = "DMM Disease Models and Mechanisms",

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T1 - Short-term and bystander effects of radiation on murine submandibular glands

AU - Uchida, Hitoshi

AU - Ingalls, Matthew H.

AU - Maruyama, Eri O.

AU - Johnston, Carl J.

AU - Hernady, Eric

AU - Faustoferri, Roberta C.

AU - Ovitt, Catherine E.

PY - 2022/11

Y1 - 2022/11

N2 - Many patients treated for head and neck cancers experience salivary gland hypofunction due to radiation damage. Understanding the mechanisms of cellular damage induced by radiation treatment is important in order to design methods of radioprotection. In addition, it is crucial to recognize the indirect effects of IR and the systemic responses that may alter saliva secretion. In this study, radiation was delivered to murine submandibular glands (SMG) bilaterally, using a 137Cs gamma ray irradiator, or unilaterally, using a small animal radiation research platform (SARRP). Analysis at 3, 24 and 48 hours showed dynamic changes in mRNA levels and protein abundance in SMG irradiated bilaterally. Unilateral irradiation using the SARRP caused similar changes in the irradiated SMG, as well as significant off-target, bystander effects in the non-irradiated contralateral SMG.

AB - Many patients treated for head and neck cancers experience salivary gland hypofunction due to radiation damage. Understanding the mechanisms of cellular damage induced by radiation treatment is important in order to design methods of radioprotection. In addition, it is crucial to recognize the indirect effects of IR and the systemic responses that may alter saliva secretion. In this study, radiation was delivered to murine submandibular glands (SMG) bilaterally, using a 137Cs gamma ray irradiator, or unilaterally, using a small animal radiation research platform (SARRP). Analysis at 3, 24 and 48 hours showed dynamic changes in mRNA levels and protein abundance in SMG irradiated bilaterally. Unilateral irradiation using the SARRP caused similar changes in the irradiated SMG, as well as significant off-target, bystander effects in the non-irradiated contralateral SMG.

KW - Salivary glands

KW - bystander effects

KW - radiation

KW - small animal radiation research platform

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SN - 1754-8403

VL - 15

JO - DMM Disease Models and Mechanisms

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IS - 11

ER -

Short-term and bystander effects of radiation on murine submandibular glands

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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