Sensitive bioluminescent detection of pesticides utilizing a membrane mutant of Escherichia coli and recombinant DNA technology

Soomi Lee; Masayasu Suzuki; Eiichi Tamiya; Isao Karube

doi:10.1016/0003-2670(92)85169-7

Sensitive bioluminescent detection of pesticides utilizing a membrane mutant of Escherichia coli and recombinant DNA technology

Soomi Lee^*, Masayasu Suzuki, Eiichi Tamiya, Isao Karube

^*Corresponding author for this work

Electric and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The development of a microbial sensing system utilizing a membrane mutant of Escherichia coli and recominant DNA technology for the determination of pesticides in the environment is described. The emission of light in E. coli was achieved by cloning the genes encoding firefly luciferase, and by injecting the luminescent substrate, luciferin. A considerable increase in sensitivity to toxic substances was obtained using the membrane mutant of E. coli as compared with the wild type. Pesticides and herbicides could be detected by the decrease of in vivo luminescence at concentrations down to 50 μg l^-1.

Original language	English
Pages (from-to)	183-188
Number of pages	6
Journal	Analytica Chimica Acta
Volume	257
Issue number	2
DOIs	https://doi.org/10.1016/0003-2670(92)85169-7
State	Published - 1992/02/25

Keywords

Biosensors
Chemiluminescence
Herbicides
Pesticides

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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abstract = "The development of a microbial sensing system utilizing a membrane mutant of Escherichia coli and recominant DNA technology for the determination of pesticides in the environment is described. The emission of light in E. coli was achieved by cloning the genes encoding firefly luciferase, and by injecting the luminescent substrate, luciferin. A considerable increase in sensitivity to toxic substances was obtained using the membrane mutant of E. coli as compared with the wild type. Pesticides and herbicides could be detected by the decrease of in vivo luminescence at concentrations down to 50 μg l-1.",

keywords = "Biosensors, Chemiluminescence, Herbicides, Pesticides",

author = "Soomi Lee and Masayasu Suzuki and Eiichi Tamiya and Isao Karube",

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T1 - Sensitive bioluminescent detection of pesticides utilizing a membrane mutant of Escherichia coli and recombinant DNA technology

AU - Lee, Soomi

AU - Suzuki, Masayasu

AU - Tamiya, Eiichi

AU - Karube, Isao

PY - 1992/2/25

Y1 - 1992/2/25

N2 - The development of a microbial sensing system utilizing a membrane mutant of Escherichia coli and recominant DNA technology for the determination of pesticides in the environment is described. The emission of light in E. coli was achieved by cloning the genes encoding firefly luciferase, and by injecting the luminescent substrate, luciferin. A considerable increase in sensitivity to toxic substances was obtained using the membrane mutant of E. coli as compared with the wild type. Pesticides and herbicides could be detected by the decrease of in vivo luminescence at concentrations down to 50 μg l-1.

AB - The development of a microbial sensing system utilizing a membrane mutant of Escherichia coli and recominant DNA technology for the determination of pesticides in the environment is described. The emission of light in E. coli was achieved by cloning the genes encoding firefly luciferase, and by injecting the luminescent substrate, luciferin. A considerable increase in sensitivity to toxic substances was obtained using the membrane mutant of E. coli as compared with the wild type. Pesticides and herbicides could be detected by the decrease of in vivo luminescence at concentrations down to 50 μg l-1.

KW - Biosensors

KW - Chemiluminescence

KW - Herbicides

KW - Pesticides

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U2 - 10.1016/0003-2670(92)85169-7

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JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2

ER -

Sensitive bioluminescent detection of pesticides utilizing a membrane mutant of Escherichia coli and recombinant DNA technology

Abstract

Keywords

ASJC Scopus subject areas

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