Requirement of neural activity for the maintenance of dopaminergic neurons in rat midbrain slice cultures

Hiroshi Katsuki; Chikako Takenaka; Toshiaki Kume; Shuji Kaneko; Akinori Akaike

doi:10.1016/S0304-3940(01)01570-1

Requirement of neural activity for the maintenance of dopaminergic neurons in rat midbrain slice cultures

Hiroshi Katsuki, Chikako Takenaka, Toshiaki Kume, Shuji Kaneko, Akinori Akaike^*

^*Corresponding author for this work

Applied Pharmacology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Chronic treatment of organotypic midbrain slice cultures with L-type Ca²⁺ channel blocker nicardipine (3-10 μM) or verapamil (10 μM) for 18 days resulted in a drastic decrease in the number of dopaminergic neurons. A voltage-dependent Na⁺ channel blocker tetrodotoxin (1 μM) was also effective in decreasing the number of dopaminergic neurons. Concurrent application of forskolin (20 μM) or dibutyryl cyclic AMP (1 mM) counteracted the effects of nicardipine and tetrodotoxin. These results suggest that spontaneous neuronal activity within midbrain slice cultures, causing Ca²⁺ influx through L-type Ca²⁺ channels that maintains intracellular cyclic AMP levels, is required for the maintenance of dopaminergic neurons.

Original language	English
Pages (from-to)	166-170
Number of pages	5
Journal	Neuroscience Letters
Volume	300
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(01)01570-1
State	Published - 2001/03/16

Keywords

Adenylyl cyclase
Membrane depolarization
Neuronal survival
Substantia nigra
Tyrosine hydroxylase
Voltage-dependent calcium channel

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0304-3940(01)01570-1

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title = "Requirement of neural activity for the maintenance of dopaminergic neurons in rat midbrain slice cultures",

abstract = "Chronic treatment of organotypic midbrain slice cultures with L-type Ca2+ channel blocker nicardipine (3-10 μM) or verapamil (10 μM) for 18 days resulted in a drastic decrease in the number of dopaminergic neurons. A voltage-dependent Na+ channel blocker tetrodotoxin (1 μM) was also effective in decreasing the number of dopaminergic neurons. Concurrent application of forskolin (20 μM) or dibutyryl cyclic AMP (1 mM) counteracted the effects of nicardipine and tetrodotoxin. These results suggest that spontaneous neuronal activity within midbrain slice cultures, causing Ca2+ influx through L-type Ca2+ channels that maintains intracellular cyclic AMP levels, is required for the maintenance of dopaminergic neurons.",

keywords = "Adenylyl cyclase, Membrane depolarization, Neuronal survival, Substantia nigra, Tyrosine hydroxylase, Voltage-dependent calcium channel",

author = "Hiroshi Katsuki and Chikako Takenaka and Toshiaki Kume and Shuji Kaneko and Akinori Akaike",

note = "Funding Information: This study was supported by Grant-in-Aid for Scientific Research from Japan Promotion of Science and Smoking Research Foundation.",

year = "2001",

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doi = "10.1016/S0304-3940(01)01570-1",

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T1 - Requirement of neural activity for the maintenance of dopaminergic neurons in rat midbrain slice cultures

AU - Katsuki, Hiroshi

AU - Takenaka, Chikako

AU - Kume, Toshiaki

AU - Kaneko, Shuji

AU - Akaike, Akinori

N1 - Funding Information: This study was supported by Grant-in-Aid for Scientific Research from Japan Promotion of Science and Smoking Research Foundation.

PY - 2001/3/16

Y1 - 2001/3/16

N2 - Chronic treatment of organotypic midbrain slice cultures with L-type Ca2+ channel blocker nicardipine (3-10 μM) or verapamil (10 μM) for 18 days resulted in a drastic decrease in the number of dopaminergic neurons. A voltage-dependent Na+ channel blocker tetrodotoxin (1 μM) was also effective in decreasing the number of dopaminergic neurons. Concurrent application of forskolin (20 μM) or dibutyryl cyclic AMP (1 mM) counteracted the effects of nicardipine and tetrodotoxin. These results suggest that spontaneous neuronal activity within midbrain slice cultures, causing Ca2+ influx through L-type Ca2+ channels that maintains intracellular cyclic AMP levels, is required for the maintenance of dopaminergic neurons.

AB - Chronic treatment of organotypic midbrain slice cultures with L-type Ca2+ channel blocker nicardipine (3-10 μM) or verapamil (10 μM) for 18 days resulted in a drastic decrease in the number of dopaminergic neurons. A voltage-dependent Na+ channel blocker tetrodotoxin (1 μM) was also effective in decreasing the number of dopaminergic neurons. Concurrent application of forskolin (20 μM) or dibutyryl cyclic AMP (1 mM) counteracted the effects of nicardipine and tetrodotoxin. These results suggest that spontaneous neuronal activity within midbrain slice cultures, causing Ca2+ influx through L-type Ca2+ channels that maintains intracellular cyclic AMP levels, is required for the maintenance of dopaminergic neurons.

KW - Adenylyl cyclase

KW - Membrane depolarization

KW - Neuronal survival

KW - Substantia nigra

KW - Tyrosine hydroxylase

KW - Voltage-dependent calcium channel

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JF - Neuroscience Letters

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ER -

Requirement of neural activity for the maintenance of dopaminergic neurons in rat midbrain slice cultures

Abstract

Keywords

ASJC Scopus subject areas

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