Regulation of TRPV1 channel activities by intracellular ATP in the absence of capsaicin

Takahiro Shimizu*, Nobuhiro Yanase, Takuto Fujii, Haruka Sakakibara, Hideki Sakai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Transient receptor potential vanilloid 1 (TRPV1) is a voltage-dependent non-selective cation channel activated by capsaicin, the main pungent ingredient of chili peppers, and noxious heat. Although TRPV1 channels produce outwardly rectifying currents even in the absence of capsaicin, little is known about the regulation mechanism of the TRPV1 currents. In the present study, we found that intracellular ATP regulates the basal activities of TRPV1 channels in a concentration-dependent manner. The ATP-dependent regulation of TRPV1 channels was mediated by phosphoinositides. Moreover, an increase in intracellular ATP concentration negatively shifted voltage-dependent activation of TRPV1 channels. These results suggest that the ATP-dependent production of phosphoinositides regulates the voltage-dependent gating of the basal TRPV1 channel activities in the absence of capsaicin.

Original languageEnglish
Article number183782
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1864
Issue number1
DOIs
StatePublished - 2022/02/01

Keywords

  • ATP
  • Channel
  • Phosphoinositide
  • TRPV1
  • Transient receptor potential
  • Voltage-dependent gating

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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