Regulation of food intake in the goldfish by interaction between ghrelin and orexin

Tohru Miura; Keisuke Maruyama; Sei Ichi Shimakura; Hiroyuki Kaiya; Minoru Uchiyama; Kenji Kangawa; Seiji Shioda; Kouhei Matsuda

doi:10.1016/j.peptides.2007.03.023

Regulation of food intake in the goldfish by interaction between ghrelin and orexin

Tohru Miura, Keisuke Maruyama, Sei Ichi Shimakura, Hiroyuki Kaiya, Minoru Uchiyama, Kenji Kangawa, Seiji Shioda, Kouhei Matsuda^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

Intracerebroventricular (ICV) administration of ghrelin, orexin and neuropeptide Y (NPY) stimulates food intake in goldfish. Orexin and NPY interact with each other in the regulation of feeding, while ghrelin-induced feeding has also shown to be mediated by NPY in the goldfish model. To investigate the interaction between ghrelin and orexin, we examined the effects of a selective orexin receptor-1 antagonist, SB334867, and a growth hormone secretagogue-receptor antagonist, [D-Lys³]-GHRP-6, on ghrelin- and orexin-A-induced feeding. Ghrelin-induced food intake was completely inhibited for 1 h following ICV preinjection of SB334867, while [D-Lys³]-GHRP-6 attenuated orexin-A stimulated feeding. Furthermore, ICV administration of ghrelin or orexin-A at a dose sufficient to stimulate food intake increased the expression of each other's mRNA in the diencephalon. These results indicate that, in goldfish, ghrelin and orexin-A have interacting orexigenic effects in the central nervous system. This is the first report that orexin-A-induced feeding is mediated by the ghrelin signaling in any animal model.

Original language	English
Pages (from-to)	1207-1213
Number of pages	7
Journal	Peptides
Volume	28
Issue number	6
DOIs	https://doi.org/10.1016/j.peptides.2007.03.023
State	Published - 2007/06

Keywords

Ghrelin
Goldfish
Orexigenic action
Orexin
SB334867
[D-Lys]-GHRP-6

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/j.peptides.2007.03.023

Cite this

@article{be6b380814094393957d0e4ebd4dbbb5,

title = "Regulation of food intake in the goldfish by interaction between ghrelin and orexin",

abstract = "Intracerebroventricular (ICV) administration of ghrelin, orexin and neuropeptide Y (NPY) stimulates food intake in goldfish. Orexin and NPY interact with each other in the regulation of feeding, while ghrelin-induced feeding has also shown to be mediated by NPY in the goldfish model. To investigate the interaction between ghrelin and orexin, we examined the effects of a selective orexin receptor-1 antagonist, SB334867, and a growth hormone secretagogue-receptor antagonist, [D-Lys3]-GHRP-6, on ghrelin- and orexin-A-induced feeding. Ghrelin-induced food intake was completely inhibited for 1 h following ICV preinjection of SB334867, while [D-Lys3]-GHRP-6 attenuated orexin-A stimulated feeding. Furthermore, ICV administration of ghrelin or orexin-A at a dose sufficient to stimulate food intake increased the expression of each other's mRNA in the diencephalon. These results indicate that, in goldfish, ghrelin and orexin-A have interacting orexigenic effects in the central nervous system. This is the first report that orexin-A-induced feeding is mediated by the ghrelin signaling in any animal model.",

keywords = "Ghrelin, Goldfish, Orexigenic action, Orexin, SB334867, [D-Lys]-GHRP-6",

author = "Tohru Miura and Keisuke Maruyama and Shimakura, {Sei Ichi} and Hiroyuki Kaiya and Minoru Uchiyama and Kenji Kangawa and Seiji Shioda and Kouhei Matsuda",

note = "Funding Information: We are grateful to the Biopharma Center of Daiichi Asubio Pharmaceuticals, Inc. for synthesizing goldfish ghrelin peptides. This work was supported by Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (K.M.), and by research grants from the University of Toyama and Toyama Marine Biotechnology Association (K.M.). ",

year = "2007",

month = jun,

doi = "10.1016/j.peptides.2007.03.023",

language = "英語",

volume = "28",

pages = "1207--1213",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "6",

}

TY - JOUR

T1 - Regulation of food intake in the goldfish by interaction between ghrelin and orexin

AU - Miura, Tohru

AU - Maruyama, Keisuke

AU - Shimakura, Sei Ichi

AU - Kaiya, Hiroyuki

AU - Uchiyama, Minoru

AU - Kangawa, Kenji

AU - Shioda, Seiji

AU - Matsuda, Kouhei

N1 - Funding Information: We are grateful to the Biopharma Center of Daiichi Asubio Pharmaceuticals, Inc. for synthesizing goldfish ghrelin peptides. This work was supported by Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (K.M.), and by research grants from the University of Toyama and Toyama Marine Biotechnology Association (K.M.).

PY - 2007/6

Y1 - 2007/6

N2 - Intracerebroventricular (ICV) administration of ghrelin, orexin and neuropeptide Y (NPY) stimulates food intake in goldfish. Orexin and NPY interact with each other in the regulation of feeding, while ghrelin-induced feeding has also shown to be mediated by NPY in the goldfish model. To investigate the interaction between ghrelin and orexin, we examined the effects of a selective orexin receptor-1 antagonist, SB334867, and a growth hormone secretagogue-receptor antagonist, [D-Lys3]-GHRP-6, on ghrelin- and orexin-A-induced feeding. Ghrelin-induced food intake was completely inhibited for 1 h following ICV preinjection of SB334867, while [D-Lys3]-GHRP-6 attenuated orexin-A stimulated feeding. Furthermore, ICV administration of ghrelin or orexin-A at a dose sufficient to stimulate food intake increased the expression of each other's mRNA in the diencephalon. These results indicate that, in goldfish, ghrelin and orexin-A have interacting orexigenic effects in the central nervous system. This is the first report that orexin-A-induced feeding is mediated by the ghrelin signaling in any animal model.

AB - Intracerebroventricular (ICV) administration of ghrelin, orexin and neuropeptide Y (NPY) stimulates food intake in goldfish. Orexin and NPY interact with each other in the regulation of feeding, while ghrelin-induced feeding has also shown to be mediated by NPY in the goldfish model. To investigate the interaction between ghrelin and orexin, we examined the effects of a selective orexin receptor-1 antagonist, SB334867, and a growth hormone secretagogue-receptor antagonist, [D-Lys3]-GHRP-6, on ghrelin- and orexin-A-induced feeding. Ghrelin-induced food intake was completely inhibited for 1 h following ICV preinjection of SB334867, while [D-Lys3]-GHRP-6 attenuated orexin-A stimulated feeding. Furthermore, ICV administration of ghrelin or orexin-A at a dose sufficient to stimulate food intake increased the expression of each other's mRNA in the diencephalon. These results indicate that, in goldfish, ghrelin and orexin-A have interacting orexigenic effects in the central nervous system. This is the first report that orexin-A-induced feeding is mediated by the ghrelin signaling in any animal model.

KW - Ghrelin

KW - Goldfish

KW - Orexigenic action

KW - Orexin

KW - SB334867

KW - [D-Lys]-GHRP-6

UR - http://www.scopus.com/inward/record.url?scp=34249750978&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2007.03.023

DO - 10.1016/j.peptides.2007.03.023

M3 - 学術論文

C2 - 17481778

AN - SCOPUS:34249750978

SN - 0196-9781

VL - 28

SP - 1207

EP - 1213

JO - Peptides

JF - Peptides

IS - 6

ER -

Regulation of food intake in the goldfish by interaction between ghrelin and orexin

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this