Reduced Stathmin-1 Expression in Natural Killer Cells Associated with Spontaneous Abortion

Yi Lin; Cui Li; Bin Shan; Wenjing Wang; Shigeru Saito; Jiehan Xu; Jingfang Di; Yanmin Zhong; Da Jin Li

doi:10.1016/j.ajpath.2010.10.005

Reduced Stathmin-1 Expression in Natural Killer Cells Associated with Spontaneous Abortion

Yi Lin^*, Cui Li, Bin Shan, Wenjing Wang, Shigeru Saito, Jiehan Xu, Jingfang Di, Yanmin Zhong, Da Jin Li

^*Corresponding author for this work

Member of the Board

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Female CBA/J mice impregnated by male DBA/2J mice (CBA/JxDBA/2J matings) are prone to spontaneous abortion, although the reason for this is unclear. In this study, the stathmin-1 expression pattern was evaluated in uterine natural killer (uNK) cells purified from CBA/JxDBA/2J matings. Results were compared with those in a CBA/JxBALB/c control group that yields successful pregnancies. The mean ± SD percentage of stathmin-1⁺ cells in the CD49b⁺ uNK cell population was lower in CBA/JxDBA/2J mice (0.7% ± 0.4%) than in control CBA/JxBALB/c mice (4.9% ± 1.5%, P < 0.01) using flow cytometry, and the intracellular stathmin-1 level in uNK cells was lower in CBA/JxDBA/2J mice than in control mice using Western blot analysis. Co-localization of lectin from Dolichos biflorus agglutinin (DBA-lectin) and stathmin-1 was confirmed using multivision immunohistochemical analysis. The frequency of stathmin-1⁺DBA-lectin⁺ cells was lower in CBA/JxDBA/2J mice than in CBA/JxBALB/c mice. A similar trend in the frequency of stathmin-1⁺CD56⁺ cells was seen in patients with unexplained spontaneous abortion compared with normal early pregnancy. A neutralizing antibody against stathmin-1 further increased the percentage of embryo loss in CBA/JxDBA/2J matings. These results provide evidence that stathmin-1 expression in uNK cells at the maternal-fetal interface may help modulate uNK cell function and may be beneficial for a successful pregnancy.

Original language	English
Pages (from-to)	506-514
Number of pages	9
Journal	American Journal of Pathology
Volume	178
Issue number	2
DOIs	https://doi.org/10.1016/j.ajpath.2010.10.005
State	Published - 2011/02

ASJC Scopus subject areas

Pathology and Forensic Medicine

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@article{26e717ecf1614c2496332f08d5b1d497,

title = "Reduced Stathmin-1 Expression in Natural Killer Cells Associated with Spontaneous Abortion",

abstract = "Female CBA/J mice impregnated by male DBA/2J mice (CBA/JxDBA/2J matings) are prone to spontaneous abortion, although the reason for this is unclear. In this study, the stathmin-1 expression pattern was evaluated in uterine natural killer (uNK) cells purified from CBA/JxDBA/2J matings. Results were compared with those in a CBA/JxBALB/c control group that yields successful pregnancies. The mean ± SD percentage of stathmin-1+ cells in the CD49b+ uNK cell population was lower in CBA/JxDBA/2J mice (0.7% ± 0.4%) than in control CBA/JxBALB/c mice (4.9% ± 1.5%, P < 0.01) using flow cytometry, and the intracellular stathmin-1 level in uNK cells was lower in CBA/JxDBA/2J mice than in control mice using Western blot analysis. Co-localization of lectin from Dolichos biflorus agglutinin (DBA-lectin) and stathmin-1 was confirmed using multivision immunohistochemical analysis. The frequency of stathmin-1+DBA-lectin+ cells was lower in CBA/JxDBA/2J mice than in CBA/JxBALB/c mice. A similar trend in the frequency of stathmin-1+CD56+ cells was seen in patients with unexplained spontaneous abortion compared with normal early pregnancy. A neutralizing antibody against stathmin-1 further increased the percentage of embryo loss in CBA/JxDBA/2J matings. These results provide evidence that stathmin-1 expression in uNK cells at the maternal-fetal interface may help modulate uNK cell function and may be beneficial for a successful pregnancy.",

author = "Yi Lin and Cui Li and Bin Shan and Wenjing Wang and Shigeru Saito and Jiehan Xu and Jingfang Di and Yanmin Zhong and Li, {Da Jin}",

note = "Funding Information: Supported by the National Natural Science Foundation of China ( 30730087 , 30872761 , and 30972970 ), the National Basic Research Program of China ( 2006CB944007 ), the Program for New Century Excellent Talents in University ( NCET-07-0861 ), the Focus Construction Subject of Shanghai Education Department, the New Cutting-Edge Technology Project ( SHDC12010122 ), a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Grant-in-Aid for Scientific Research [B] - 20390431 ), and grants from the Ministry of Health Labour and Welfare, Japan (Health Labour Sciences Research grant H20-kodomo-ippan-002 ). ",

year = "2011",

month = feb,

doi = "10.1016/j.ajpath.2010.10.005",

language = "英語",

volume = "178",

pages = "506--514",

journal = "American Journal of Pathology",

issn = "0002-9440",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Reduced Stathmin-1 Expression in Natural Killer Cells Associated with Spontaneous Abortion

AU - Lin, Yi

AU - Li, Cui

AU - Shan, Bin

AU - Wang, Wenjing

AU - Saito, Shigeru

AU - Xu, Jiehan

AU - Di, Jingfang

AU - Zhong, Yanmin

AU - Li, Da Jin

N1 - Funding Information: Supported by the National Natural Science Foundation of China ( 30730087 , 30872761 , and 30972970 ), the National Basic Research Program of China ( 2006CB944007 ), the Program for New Century Excellent Talents in University ( NCET-07-0861 ), the Focus Construction Subject of Shanghai Education Department, the New Cutting-Edge Technology Project ( SHDC12010122 ), a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Grant-in-Aid for Scientific Research [B] - 20390431 ), and grants from the Ministry of Health Labour and Welfare, Japan (Health Labour Sciences Research grant H20-kodomo-ippan-002 ).

PY - 2011/2

Y1 - 2011/2

N2 - Female CBA/J mice impregnated by male DBA/2J mice (CBA/JxDBA/2J matings) are prone to spontaneous abortion, although the reason for this is unclear. In this study, the stathmin-1 expression pattern was evaluated in uterine natural killer (uNK) cells purified from CBA/JxDBA/2J matings. Results were compared with those in a CBA/JxBALB/c control group that yields successful pregnancies. The mean ± SD percentage of stathmin-1+ cells in the CD49b+ uNK cell population was lower in CBA/JxDBA/2J mice (0.7% ± 0.4%) than in control CBA/JxBALB/c mice (4.9% ± 1.5%, P < 0.01) using flow cytometry, and the intracellular stathmin-1 level in uNK cells was lower in CBA/JxDBA/2J mice than in control mice using Western blot analysis. Co-localization of lectin from Dolichos biflorus agglutinin (DBA-lectin) and stathmin-1 was confirmed using multivision immunohistochemical analysis. The frequency of stathmin-1+DBA-lectin+ cells was lower in CBA/JxDBA/2J mice than in CBA/JxBALB/c mice. A similar trend in the frequency of stathmin-1+CD56+ cells was seen in patients with unexplained spontaneous abortion compared with normal early pregnancy. A neutralizing antibody against stathmin-1 further increased the percentage of embryo loss in CBA/JxDBA/2J matings. These results provide evidence that stathmin-1 expression in uNK cells at the maternal-fetal interface may help modulate uNK cell function and may be beneficial for a successful pregnancy.

AB - Female CBA/J mice impregnated by male DBA/2J mice (CBA/JxDBA/2J matings) are prone to spontaneous abortion, although the reason for this is unclear. In this study, the stathmin-1 expression pattern was evaluated in uterine natural killer (uNK) cells purified from CBA/JxDBA/2J matings. Results were compared with those in a CBA/JxBALB/c control group that yields successful pregnancies. The mean ± SD percentage of stathmin-1+ cells in the CD49b+ uNK cell population was lower in CBA/JxDBA/2J mice (0.7% ± 0.4%) than in control CBA/JxBALB/c mice (4.9% ± 1.5%, P < 0.01) using flow cytometry, and the intracellular stathmin-1 level in uNK cells was lower in CBA/JxDBA/2J mice than in control mice using Western blot analysis. Co-localization of lectin from Dolichos biflorus agglutinin (DBA-lectin) and stathmin-1 was confirmed using multivision immunohistochemical analysis. The frequency of stathmin-1+DBA-lectin+ cells was lower in CBA/JxDBA/2J mice than in CBA/JxBALB/c mice. A similar trend in the frequency of stathmin-1+CD56+ cells was seen in patients with unexplained spontaneous abortion compared with normal early pregnancy. A neutralizing antibody against stathmin-1 further increased the percentage of embryo loss in CBA/JxDBA/2J matings. These results provide evidence that stathmin-1 expression in uNK cells at the maternal-fetal interface may help modulate uNK cell function and may be beneficial for a successful pregnancy.

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DO - 10.1016/j.ajpath.2010.10.005

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AN - SCOPUS:79951838030

SN - 0002-9440

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JO - American Journal of Pathology

JF - American Journal of Pathology

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ER -

Reduced Stathmin-1 Expression in Natural Killer Cells Associated with Spontaneous Abortion

Abstract

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