Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)

Shin Yamamoto; Yusuke Oshima; Takashi Saitou; Takao Watanabe; Teruki Miyake; Osamu Yoshida; Yoshio Tokumoto; Masanori Abe; Bunzo Matsuura; Yoichi Hiasa; Takeshi Imamura

doi:10.1016/j.bbrep.2016.09.010

Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)

Shin Yamamoto, Yusuke Oshima, Takashi Saitou, Takao Watanabe, Teruki Miyake, Osamu Yoshida, Yoshio Tokumoto, Masanori Abe, Bunzo Matsuura, Yoichi Hiasa, Takeshi Imamura^*

^*Corresponding author for this work

Intellectual Information Engineering

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Non-alcoholic steatohepatitis (NASH) is a common liver disorder caused by fatty liver. Because NASH is associated with fibrotic and morphological changes in liver tissue, a direct imaging technique is required for accurate staging of liver tissue. For this purpose, in this study we took advantage of two label-free optical imaging techniques, second harmonic generation (SHG) and auto-fluorescence (AF), using two-photon excitation microscopy (TPEM). Three-dimensional ex vivo imaging of tissues from NASH model mice, followed by image processing, revealed that SHG and AF are sufficient to quantitatively characterize the hepatic capsule at an early stage and parenchymal morphologies associated with liver disease progression, respectively.

Original language	English
Pages (from-to)	277-283
Number of pages	7
Journal	Biochemistry and Biophysics Reports
Volume	8
DOIs	https://doi.org/10.1016/j.bbrep.2016.09.010
State	Published - 2016/12/01

Keywords

Collagen
Fibrosis
Fluorescence imaging
NASH
SHG
Two-photon excitation microscopy

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/j.bbrep.2016.09.010

Fingerprint

Dive into the research topics of 'Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)'. Together they form a unique fingerprint.

Cite this

Yamamoto, S., Oshima, Y., Saitou, T., Watanabe, T., Miyake, T., Yoshida, O., Tokumoto, Y., Abe, M., Matsuura, B., Hiasa, Y., & Imamura, T. (2016). Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM). Biochemistry and Biophysics Reports, 8, 277-283. https://doi.org/10.1016/j.bbrep.2016.09.010

@article{47d97109108b440ba50000896b974d18,

title = "Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)",

abstract = "Non-alcoholic steatohepatitis (NASH) is a common liver disorder caused by fatty liver. Because NASH is associated with fibrotic and morphological changes in liver tissue, a direct imaging technique is required for accurate staging of liver tissue. For this purpose, in this study we took advantage of two label-free optical imaging techniques, second harmonic generation (SHG) and auto-fluorescence (AF), using two-photon excitation microscopy (TPEM). Three-dimensional ex vivo imaging of tissues from NASH model mice, followed by image processing, revealed that SHG and AF are sufficient to quantitatively characterize the hepatic capsule at an early stage and parenchymal morphologies associated with liver disease progression, respectively.",

keywords = "Collagen, Fibrosis, Fluorescence imaging, NASH, SHG, Two-photon excitation microscopy",

author = "Shin Yamamoto and Yusuke Oshima and Takashi Saitou and Takao Watanabe and Teruki Miyake and Osamu Yoshida and Yoshio Tokumoto and Masanori Abe and Bunzo Matsuura and Yoichi Hiasa and Takeshi Imamura",

note = "Publisher Copyright: {\textcopyright} 2016 The Authors",

year = "2016",

month = dec,

day = "1",

doi = "10.1016/j.bbrep.2016.09.010",

language = "英語",

volume = "8",

pages = "277--283",

journal = "Biochemistry and Biophysics Reports",

issn = "2405-5808",

publisher = "Elsevier B.V.",

}

Yamamoto, S, Oshima, Y, Saitou, T, Watanabe, T, Miyake, T, Yoshida, O, Tokumoto, Y, Abe, M, Matsuura, B, Hiasa, Y & Imamura, T 2016, 'Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)', Biochemistry and Biophysics Reports, vol. 8, pp. 277-283. https://doi.org/10.1016/j.bbrep.2016.09.010

Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM). / Yamamoto, Shin; Oshima, Yusuke; Saitou, Takashi et al.
In: Biochemistry and Biophysics Reports, Vol. 8, 01.12.2016, p. 277-283.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)

AU - Yamamoto, Shin

AU - Oshima, Yusuke

AU - Saitou, Takashi

AU - Watanabe, Takao

AU - Miyake, Teruki

AU - Yoshida, Osamu

AU - Tokumoto, Yoshio

AU - Abe, Masanori

AU - Matsuura, Bunzo

AU - Hiasa, Yoichi

AU - Imamura, Takeshi

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Non-alcoholic steatohepatitis (NASH) is a common liver disorder caused by fatty liver. Because NASH is associated with fibrotic and morphological changes in liver tissue, a direct imaging technique is required for accurate staging of liver tissue. For this purpose, in this study we took advantage of two label-free optical imaging techniques, second harmonic generation (SHG) and auto-fluorescence (AF), using two-photon excitation microscopy (TPEM). Three-dimensional ex vivo imaging of tissues from NASH model mice, followed by image processing, revealed that SHG and AF are sufficient to quantitatively characterize the hepatic capsule at an early stage and parenchymal morphologies associated with liver disease progression, respectively.

AB - Non-alcoholic steatohepatitis (NASH) is a common liver disorder caused by fatty liver. Because NASH is associated with fibrotic and morphological changes in liver tissue, a direct imaging technique is required for accurate staging of liver tissue. For this purpose, in this study we took advantage of two label-free optical imaging techniques, second harmonic generation (SHG) and auto-fluorescence (AF), using two-photon excitation microscopy (TPEM). Three-dimensional ex vivo imaging of tissues from NASH model mice, followed by image processing, revealed that SHG and AF are sufficient to quantitatively characterize the hepatic capsule at an early stage and parenchymal morphologies associated with liver disease progression, respectively.

KW - Collagen

KW - Fibrosis

KW - Fluorescence imaging

KW - NASH

KW - SHG

KW - Two-photon excitation microscopy

UR - http://www.scopus.com/inward/record.url?scp=84991573196&partnerID=8YFLogxK

U2 - 10.1016/j.bbrep.2016.09.010

DO - 10.1016/j.bbrep.2016.09.010

M3 - 学術論文

C2 - 28955967

AN - SCOPUS:84991573196

SN - 2405-5808

VL - 8

SP - 277

EP - 283

JO - Biochemistry and Biophysics Reports

JF - Biochemistry and Biophysics Reports

ER -

Yamamoto S, Oshima Y, Saitou T, Watanabe T, Miyake T, Yoshida O et al. Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM). Biochemistry and Biophysics Reports. 2016 Dec 1;8:277-283. doi: 10.1016/j.bbrep.2016.09.010