Preparation and characterization of protein Langmuir-Blodgett films

K. Owaku; H. Shinohara; Y. Ikariyama; M. Aizawa

doi:10.1016/0040-6090(89)90054-0

Preparation and characterization of protein Langmuir-Blodgett films

K. Owaku^*, H. Shinohara, Y. Ikariyama, M. Aizawa

^*Corresponding author for this work

Life Sciences and Bioengineering

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A protein monolayer of bovin serum albumin (BSA) was stabilized at the air- water interface using glutaraldehyde (GA). The GA-treated BSA film was transferred onto the glass surface, followed by immunochemical lamination of anti-BSA antibody. Protein A-β-galactosidase (pro A-β-gal) conjugate was then reacted with the antibody molecular layer. Biofunctional protein films can be prepared on the BSA base Langmuir-Blodgett film by intermolecular complementary affinity between the proteins concerned.

Original language	English
Pages (from-to)	61-64
Number of pages	4
Journal	Thin Solid Films
Volume	180
Issue number	1-2
DOIs	https://doi.org/10.1016/0040-6090(89)90054-0
State	Published - 1989/11/21

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Surfaces and Interfaces
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Preparation and characterization of protein Langmuir-Blodgett films",

abstract = "A protein monolayer of bovin serum albumin (BSA) was stabilized at the air- water interface using glutaraldehyde (GA). The GA-treated BSA film was transferred onto the glass surface, followed by immunochemical lamination of anti-BSA antibody. Protein A-β-galactosidase (pro A-β-gal) conjugate was then reacted with the antibody molecular layer. Biofunctional protein films can be prepared on the BSA base Langmuir-Blodgett film by intermolecular complementary affinity between the proteins concerned.",

author = "K. Owaku and H. Shinohara and Y. Ikariyama and M. Aizawa",

year = "1989",

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doi = "10.1016/0040-6090(89)90054-0",

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AU - Owaku, K.

AU - Shinohara, H.

AU - Ikariyama, Y.

AU - Aizawa, M.

PY - 1989/11/21

Y1 - 1989/11/21

N2 - A protein monolayer of bovin serum albumin (BSA) was stabilized at the air- water interface using glutaraldehyde (GA). The GA-treated BSA film was transferred onto the glass surface, followed by immunochemical lamination of anti-BSA antibody. Protein A-β-galactosidase (pro A-β-gal) conjugate was then reacted with the antibody molecular layer. Biofunctional protein films can be prepared on the BSA base Langmuir-Blodgett film by intermolecular complementary affinity between the proteins concerned.

AB - A protein monolayer of bovin serum albumin (BSA) was stabilized at the air- water interface using glutaraldehyde (GA). The GA-treated BSA film was transferred onto the glass surface, followed by immunochemical lamination of anti-BSA antibody. Protein A-β-galactosidase (pro A-β-gal) conjugate was then reacted with the antibody molecular layer. Biofunctional protein films can be prepared on the BSA base Langmuir-Blodgett film by intermolecular complementary affinity between the proteins concerned.

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U2 - 10.1016/0040-6090(89)90054-0

DO - 10.1016/0040-6090(89)90054-0

M3 - 学術論文

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EP - 64

JO - Thin Solid Films

JF - Thin Solid Films

IS - 1-2

ER -

Preparation and characterization of protein Langmuir-Blodgett films

Abstract

ASJC Scopus subject areas

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