Polymyxin B binds to anandamide and inhibits its cytotoxic effect

Yin Wang; Yan Liu; Krishna Pada Sarker; Mihoko Nakashima; Takeshi Serizawa; Akio Kishida; Mitsuru Akashi; Masanori Nakata; Isao Kitajima; Ikuro Maruyama

doi:10.1016/S0014-5793(00)01313-2

Polymyxin B binds to anandamide and inhibits its cytotoxic effect

Yin Wang, Yan Liu, Krishna Pada Sarker, Mihoko Nakashima, Takeshi Serizawa, Akio Kishida, Mitsuru Akashi, Masanori Nakata, Isao Kitajima, Ikuro Maruyama^*

^*Corresponding author for this work

Member of the Board

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Abstract

Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to be a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol. We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	151-155
Number of pages	5
Journal	FEBS Letters
Volume	470
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(00)01313-2
State	Published - 2000/03/24

Keywords

Anandamide
Cannabinoid
Endotoxin shock
Polymyxin B
Surface plasmon resonance

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(00)01313-2

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title = "Polymyxin B binds to anandamide and inhibits its cytotoxic effect",

abstract = "Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to be a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol. We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock. Copyright (C) 2000 Federation of European Biochemical Societies.",

keywords = "Anandamide, Cannabinoid, Endotoxin shock, Polymyxin B, Surface plasmon resonance",

author = "Yin Wang and Yan Liu and Sarker, {Krishna Pada} and Mihoko Nakashima and Takeshi Serizawa and Akio Kishida and Mitsuru Akashi and Masanori Nakata and Isao Kitajima and Ikuro Maruyama",

year = "2000",

month = mar,

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doi = "10.1016/S0014-5793(00)01313-2",

language = "英語",

volume = "470",

pages = "151--155",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Polymyxin B binds to anandamide and inhibits its cytotoxic effect

AU - Wang, Yin

AU - Liu, Yan

AU - Sarker, Krishna Pada

AU - Nakashima, Mihoko

AU - Serizawa, Takeshi

AU - Kishida, Akio

AU - Akashi, Mitsuru

AU - Nakata, Masanori

AU - Kitajima, Isao

AU - Maruyama, Ikuro

PY - 2000/3/24

Y1 - 2000/3/24

N2 - Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to be a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol. We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to be a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol. We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock. Copyright (C) 2000 Federation of European Biochemical Societies.

KW - Anandamide

KW - Cannabinoid

KW - Endotoxin shock

KW - Polymyxin B

KW - Surface plasmon resonance

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U2 - 10.1016/S0014-5793(00)01313-2

DO - 10.1016/S0014-5793(00)01313-2

M3 - 学術論文

C2 - 10734225

AN - SCOPUS:0034708407

SN - 0014-5793

VL - 470

SP - 151

EP - 155

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Polymyxin B binds to anandamide and inhibits its cytotoxic effect

Abstract

Keywords

ASJC Scopus subject areas

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