Polymer brush with pendent glucosylurea groups constructed on a glass substrate by RAFT polymerization

Hiromi Kitano; Yan Liu; Ken Ichi Tokuwa; Lifu Li; Shintaroh Iwanaga; Masato Nakamura; Naoki Kanayama; Kohji Ohno; Yoshiyuki Saruwatari

doi:10.1016/j.eurpolymj.2012.08.011

Polymer brush with pendent glucosylurea groups constructed on a glass substrate by RAFT polymerization

Hiromi Kitano^*, Yan Liu, Ken Ichi Tokuwa, Lifu Li, Shintaroh Iwanaga, Masato Nakamura, Naoki Kanayama, Kohji Ohno, Yoshiyuki Saruwatari

^*Corresponding author for this work

Life Sciences and Bioengineering

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A polymer brush with pendent d-glucosylurea groups (Poly(glucosylureaethyl methacrylate), PolyGUMA) was obtained by surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization of GUMA on a glass substrate. The linear relationship between the log _e ([M] _o/[M]) value and the reaction conversion, and the small polydispersity in M _w/M _n value of PolyGUMA produced in liquid phase at the same time suggested that the polymerization proceeded in a living manner. The bicinchoninic acid method indicated that the PolyGUMA brush was significantly resistant against non-specific adsorption of albumin (bovine serum) and lysozyme (egg white). Furthermore, the adhesion of cells such as HepG2, and HEK293 cells was strongly suppressed by the presence of PolyGUMA brush. The glucosylurea group-carrying polymer brush prepared here might be quite useful to provide a "bio-inert (anti-biofouling)" surface in bio-medical fields.

Original language	English
Pages (from-to)	1875-1882
Number of pages	8
Journal	European Polymer Journal
Volume	48
Issue number	11
DOIs	https://doi.org/10.1016/j.eurpolymj.2012.08.011
State	Published - 2012/11

Keywords

Anti-biofouling
Cell adhesion
Polyglucosylurea brush
Protein adsorption
RAFT

ASJC Scopus subject areas

General Physics and Astronomy
Polymers and Plastics
Organic Chemistry
Materials Chemistry

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10.1016/j.eurpolymj.2012.08.011

Cite this

@article{e6360d55affe492d9fb5387a5a35faf9,

title = "Polymer brush with pendent glucosylurea groups constructed on a glass substrate by RAFT polymerization",

abstract = "A polymer brush with pendent d-glucosylurea groups (Poly(glucosylureaethyl methacrylate), PolyGUMA) was obtained by surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization of GUMA on a glass substrate. The linear relationship between the log e ([M] o/[M]) value and the reaction conversion, and the small polydispersity in M w/M n value of PolyGUMA produced in liquid phase at the same time suggested that the polymerization proceeded in a living manner. The bicinchoninic acid method indicated that the PolyGUMA brush was significantly resistant against non-specific adsorption of albumin (bovine serum) and lysozyme (egg white). Furthermore, the adhesion of cells such as HepG2, and HEK293 cells was strongly suppressed by the presence of PolyGUMA brush. The glucosylurea group-carrying polymer brush prepared here might be quite useful to provide a {"}bio-inert (anti-biofouling){"} surface in bio-medical fields.",

keywords = "Anti-biofouling, Cell adhesion, Polyglucosylurea brush, Protein adsorption, RAFT",

author = "Hiromi Kitano and Yan Liu and Tokuwa, {Ken Ichi} and Lifu Li and Shintaroh Iwanaga and Masato Nakamura and Naoki Kanayama and Kohji Ohno and Yoshiyuki Saruwatari",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (22350101) from the Japan Society for the Promotion of Science (JSPS) and a Grant-in-Aid for Scientific Research on Innovative Areas (20106007) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT). The authors wish to thank Professor Y. Tsujii, Kyoto University, for allowing us to use the ellipsometer. The authors are grateful to Messeurs Takuya Kondo and Sho Saito of the Department for their technical assistances. The authors are indebted to Osaka Organic Chemical Industry for the continuous support. ",

year = "2012",

month = nov,

doi = "10.1016/j.eurpolymj.2012.08.011",

language = "英語",

volume = "48",

pages = "1875--1882",

journal = "European Polymer Journal",

issn = "0014-3057",

publisher = "Elsevier Ltd.",

number = "11",

}

TY - JOUR

T1 - Polymer brush with pendent glucosylurea groups constructed on a glass substrate by RAFT polymerization

AU - Kitano, Hiromi

AU - Liu, Yan

AU - Tokuwa, Ken Ichi

AU - Li, Lifu

AU - Iwanaga, Shintaroh

AU - Nakamura, Masato

AU - Kanayama, Naoki

AU - Ohno, Kohji

AU - Saruwatari, Yoshiyuki

N1 - Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (22350101) from the Japan Society for the Promotion of Science (JSPS) and a Grant-in-Aid for Scientific Research on Innovative Areas (20106007) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT). The authors wish to thank Professor Y. Tsujii, Kyoto University, for allowing us to use the ellipsometer. The authors are grateful to Messeurs Takuya Kondo and Sho Saito of the Department for their technical assistances. The authors are indebted to Osaka Organic Chemical Industry for the continuous support.

PY - 2012/11

Y1 - 2012/11

N2 - A polymer brush with pendent d-glucosylurea groups (Poly(glucosylureaethyl methacrylate), PolyGUMA) was obtained by surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization of GUMA on a glass substrate. The linear relationship between the log e ([M] o/[M]) value and the reaction conversion, and the small polydispersity in M w/M n value of PolyGUMA produced in liquid phase at the same time suggested that the polymerization proceeded in a living manner. The bicinchoninic acid method indicated that the PolyGUMA brush was significantly resistant against non-specific adsorption of albumin (bovine serum) and lysozyme (egg white). Furthermore, the adhesion of cells such as HepG2, and HEK293 cells was strongly suppressed by the presence of PolyGUMA brush. The glucosylurea group-carrying polymer brush prepared here might be quite useful to provide a "bio-inert (anti-biofouling)" surface in bio-medical fields.

AB - A polymer brush with pendent d-glucosylurea groups (Poly(glucosylureaethyl methacrylate), PolyGUMA) was obtained by surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization of GUMA on a glass substrate. The linear relationship between the log e ([M] o/[M]) value and the reaction conversion, and the small polydispersity in M w/M n value of PolyGUMA produced in liquid phase at the same time suggested that the polymerization proceeded in a living manner. The bicinchoninic acid method indicated that the PolyGUMA brush was significantly resistant against non-specific adsorption of albumin (bovine serum) and lysozyme (egg white). Furthermore, the adhesion of cells such as HepG2, and HEK293 cells was strongly suppressed by the presence of PolyGUMA brush. The glucosylurea group-carrying polymer brush prepared here might be quite useful to provide a "bio-inert (anti-biofouling)" surface in bio-medical fields.

KW - Anti-biofouling

KW - Cell adhesion

KW - Polyglucosylurea brush

KW - Protein adsorption

KW - RAFT

UR - http://www.scopus.com/inward/record.url?scp=84867015345&partnerID=8YFLogxK

U2 - 10.1016/j.eurpolymj.2012.08.011

DO - 10.1016/j.eurpolymj.2012.08.011

M3 - 学術論文

AN - SCOPUS:84867015345

SN - 0014-3057

VL - 48

SP - 1875

EP - 1882

JO - European Polymer Journal

JF - European Polymer Journal

IS - 11

ER -

Polymer brush with pendent glucosylurea groups constructed on a glass substrate by RAFT polymerization

Abstract

Keywords

ASJC Scopus subject areas

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