TY - JOUR
T1 - Pituitary adenylate cyclase-activating polypeptide induces somatolactin release from cultured goldfish pituitary cells
AU - Azuma, Morio
AU - Tanaka, Mio
AU - Nejigaki, Yumika
AU - Uchiyama, Minoru
AU - Takahashi, Akiyoshi
AU - Shioda, Seiji
AU - Matsuda, Kouhei
N1 - Funding Information:
This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (K.M. and A.T.), and by research grants from the Faculty of Science, University of Toyama (K.M.) and from the Toyama Marine Biotechnology Association (K.M.).
PY - 2009/7
Y1 - 2009/7
N2 - In the goldfish pituitary, nerve fibers containing pituitary adenylate cyclase-activating polypeptide (PACAP) are located in close proximity to somatolactin (SL)-producing cells, and PACAP enhances SL release from cultured pituitary cells. However, there is little information about the mechanism of PACAP-induced SL release. In order to elucidate this issue, we used the cell immunoblot method. Treatment with PACAP at 10-8 and 10-7 M, but not with vasoactive intestinal polypeptide (VIP) at the same concentrations, increased the immunoblot area for SL-like immunoreactivity from dispersed pituitary cells, and PACAP-induced SL release was blocked by treatment with the PACAP selective receptor (PAC1R) antagonist, PACAP(6-38), at 10-6 M, but not with the PACAP/VIP receptor antagonist, VIP(6-28). PACAP-induced SL release was also attenuated by treatment with the calmodulin inhibitor, calmidazolium at 10-6 M. This led us to explore the signal transduction mechanism up to SL release, and we examined whether PACAP-induced SL release is mediated by the adenylate cyclase (AC)/cAMP/protein kinase A (PKA)- or the phospholipase C (PLC)/inositol 1,4,5-trisphosphate (IP3)/protein kinase C (PKC)-signaling pathway. PACAP-induced SL release was attenuated by treatment with the AC inhibitor, MDL-12330A, at 10-5 M or with the PKA inhibitor, H-89, at 10-5 M. PACAP-induced SL release was suppressed by treatment with the PLC inhibitor, U-73122, at 3 × 10-6 M or with the PKC inhibitor, GF109203X, at 10-6 M. These results suggest that PACAP can potentially function as a hypophysiotropic factor mediating SL release via the PAC1R and subsequently through perhaps the AC/cAMP/PKA- and the PLC/IP3/PKC-signaling pathways in goldfish pituitary cells.
AB - In the goldfish pituitary, nerve fibers containing pituitary adenylate cyclase-activating polypeptide (PACAP) are located in close proximity to somatolactin (SL)-producing cells, and PACAP enhances SL release from cultured pituitary cells. However, there is little information about the mechanism of PACAP-induced SL release. In order to elucidate this issue, we used the cell immunoblot method. Treatment with PACAP at 10-8 and 10-7 M, but not with vasoactive intestinal polypeptide (VIP) at the same concentrations, increased the immunoblot area for SL-like immunoreactivity from dispersed pituitary cells, and PACAP-induced SL release was blocked by treatment with the PACAP selective receptor (PAC1R) antagonist, PACAP(6-38), at 10-6 M, but not with the PACAP/VIP receptor antagonist, VIP(6-28). PACAP-induced SL release was also attenuated by treatment with the calmodulin inhibitor, calmidazolium at 10-6 M. This led us to explore the signal transduction mechanism up to SL release, and we examined whether PACAP-induced SL release is mediated by the adenylate cyclase (AC)/cAMP/protein kinase A (PKA)- or the phospholipase C (PLC)/inositol 1,4,5-trisphosphate (IP3)/protein kinase C (PKC)-signaling pathway. PACAP-induced SL release was attenuated by treatment with the AC inhibitor, MDL-12330A, at 10-5 M or with the PKA inhibitor, H-89, at 10-5 M. PACAP-induced SL release was suppressed by treatment with the PLC inhibitor, U-73122, at 3 × 10-6 M or with the PKC inhibitor, GF109203X, at 10-6 M. These results suggest that PACAP can potentially function as a hypophysiotropic factor mediating SL release via the PAC1R and subsequently through perhaps the AC/cAMP/PKA- and the PLC/IP3/PKC-signaling pathways in goldfish pituitary cells.
KW - Cell immunoblot
KW - Goldfish
KW - Hypophysiotropic factor
KW - PACAP
KW - PACR
KW - Pituitary
KW - Signal transduction
KW - Somatolactin
UR - http://www.scopus.com/inward/record.url?scp=67349241391&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2009.03.011
DO - 10.1016/j.peptides.2009.03.011
M3 - 学術論文
C2 - 19540424
AN - SCOPUS:67349241391
SN - 0196-9781
VL - 30
SP - 1260
EP - 1266
JO - Peptides
JF - Peptides
IS - 7
ER -