TY - JOUR
T1 - Photomechanical ablation of biological tissue induced by focused femtosecond laser and its application for acupuncture
AU - Hosokawa, Yoichiroh
AU - Ohta, Mika
AU - Ito, Akihiko
AU - Takaoka, Yutaka
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid for Scientific Research (Innovative Areas) (Grant No. 22120010) from the Japan Ministry of Education, Culture, Sports, Science, and Technology (MEXT) to Y.H.; a Grant-in-Aid for Challenging Exploratory Research (Grant No. 22657041) from MEXT to Y.H. and A.I.; and a Grant-in-Aid for Scientific Research (C) (Grant No. 22590653) from the Japan Society for the Promotion of Science to Y.T.
PY - 2013/3
Y1 - 2013/3
N2 - Photomechanical laser ablation due to focused femtosecond laser irradiation was induced on the hind legs of living mice, and its clinical influence on muscle cell proliferation was investigated via histological examination and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis to examine the expression of the gene encoding myostatin, which is a growth repressor in muscle satellite cells. The histological examination suggested that damage of the tissue due to the femtosecond laser irradiation was localized on epidermis and dermis and hardly induced in the muscle tissue below. On the other hand, gene expression of the myostatin of muscle tissue after laser irradiation was suppressed. The suppression of myostatin expression facilitates the proliferation of muscle cells, because myostatin is a growth repressor in muscle satellite cells. On the basis of these results, we recognize the potential of the femtosecond laser as a tool for noncontact, high-throughput acupuncturein the treatment of muscle disease.
AB - Photomechanical laser ablation due to focused femtosecond laser irradiation was induced on the hind legs of living mice, and its clinical influence on muscle cell proliferation was investigated via histological examination and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis to examine the expression of the gene encoding myostatin, which is a growth repressor in muscle satellite cells. The histological examination suggested that damage of the tissue due to the femtosecond laser irradiation was localized on epidermis and dermis and hardly induced in the muscle tissue below. On the other hand, gene expression of the myostatin of muscle tissue after laser irradiation was suppressed. The suppression of myostatin expression facilitates the proliferation of muscle cells, because myostatin is a growth repressor in muscle satellite cells. On the basis of these results, we recognize the potential of the femtosecond laser as a tool for noncontact, high-throughput acupuncturein the treatment of muscle disease.
UR - http://www.scopus.com/inward/record.url?scp=84894453163&partnerID=8YFLogxK
U2 - 10.1007/s00339-012-7138-5
DO - 10.1007/s00339-012-7138-5
M3 - 学術論文
AN - SCOPUS:84894453163
SN - 0947-8396
VL - 110
SP - 613
EP - 616
JO - Applied Physics A: Materials Science and Processing
JF - Applied Physics A: Materials Science and Processing
IS - 3
ER -