Ovine corticotropin-releasing hormone (oCRH) exerts an anxiogenic-like action in the goldfish, Carassius auratus

Kouhei Matsuda; Yasunari Hagiwara; Haruki Shibata; Atsushi Sakashita; Kohei Wada

doi:10.1016/j.ygcen.2013.01.001

Ovine corticotropin-releasing hormone (oCRH) exerts an anxiogenic-like action in the goldfish, Carassius auratus

Kouhei Matsuda^*, Yasunari Hagiwara, Haruki Shibata, Atsushi Sakashita, Kohei Wada

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Corticotropin-releasing hormone (CRH) is a member of the hypothalamic neuropeptide family that includes urocortins, urotensin I and sauvagine in vertebrates. CRH and urocortin act as anorexigenic factors for satiety regulation in rodents. In a goldfish model, intracerebroventricular (ICV) administration of ovine CRH (oCRH) affects not only food intake, but also locomotor activity. However, there is no information regarding the psychophysiological roles of CRH in goldfish. Therefore, we investigated the effect of oCRH on psychomotor activity in this species. ICV administration of synthetic oCRH at 20pmol/g body weight (BW) enhanced locomotor activity. Since intact goldfish prefer the lower to the upper area of a tank, we developed a method for measuring the time taken for fish to move from the lower to the upper area. ICV administration of oCRH at 20pmol/g BW and the central-type benzodiazepine receptor inverse agonist FG-7142 (an anxiogenic agent) at 1-4pmol/g BW both increased the time taken to move from the lower to the upper area. This anxiogenic-like effect of oCRH was abolished by the CRH receptor antagonist α-helical CRH_(9-41) (100pmol/g BW). These results indicate that CRH can potently affect locomotor and psychomotor activities in goldfish.

Original language	English
Pages (from-to)	118-122
Number of pages	5
Journal	General and Comparative Endocrinology
Volume	188
Issue number	1
DOIs	https://doi.org/10.1016/j.ygcen.2013.01.001
State	Published - 2012/08/21

Keywords

Anxiogenic-like action
CRH
Goldfish
ICV injection
Locomotor activity
Psychomotor activity

ASJC Scopus subject areas

Animal Science and Zoology
Endocrinology

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10.1016/j.ygcen.2013.01.001

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title = "Ovine corticotropin-releasing hormone (oCRH) exerts an anxiogenic-like action in the goldfish, Carassius auratus",

abstract = "Corticotropin-releasing hormone (CRH) is a member of the hypothalamic neuropeptide family that includes urocortins, urotensin I and sauvagine in vertebrates. CRH and urocortin act as anorexigenic factors for satiety regulation in rodents. In a goldfish model, intracerebroventricular (ICV) administration of ovine CRH (oCRH) affects not only food intake, but also locomotor activity. However, there is no information regarding the psychophysiological roles of CRH in goldfish. Therefore, we investigated the effect of oCRH on psychomotor activity in this species. ICV administration of synthetic oCRH at 20pmol/g body weight (BW) enhanced locomotor activity. Since intact goldfish prefer the lower to the upper area of a tank, we developed a method for measuring the time taken for fish to move from the lower to the upper area. ICV administration of oCRH at 20pmol/g BW and the central-type benzodiazepine receptor inverse agonist FG-7142 (an anxiogenic agent) at 1-4pmol/g BW both increased the time taken to move from the lower to the upper area. This anxiogenic-like effect of oCRH was abolished by the CRH receptor antagonist α-helical CRH(9-41) (100pmol/g BW). These results indicate that CRH can potently affect locomotor and psychomotor activities in goldfish.",

keywords = "Anxiogenic-like action, CRH, Goldfish, ICV injection, Locomotor activity, Psychomotor activity",

author = "Kouhei Matsuda and Yasunari Hagiwara and Haruki Shibata and Atsushi Sakashita and Kohei Wada",

note = "Funding Information: This work was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science (21370025 to K.M.), and by research grants from the University of Toyama (K.M.).",

year = "2012",

month = aug,

day = "21",

doi = "10.1016/j.ygcen.2013.01.001",

language = "英語",

volume = "188",

pages = "118--122",

journal = "General and Comparative Endocrinology",

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T1 - Ovine corticotropin-releasing hormone (oCRH) exerts an anxiogenic-like action in the goldfish, Carassius auratus

AU - Matsuda, Kouhei

AU - Hagiwara, Yasunari

AU - Shibata, Haruki

AU - Sakashita, Atsushi

AU - Wada, Kohei

N1 - Funding Information: This work was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science (21370025 to K.M.), and by research grants from the University of Toyama (K.M.).

PY - 2012/8/21

Y1 - 2012/8/21

N2 - Corticotropin-releasing hormone (CRH) is a member of the hypothalamic neuropeptide family that includes urocortins, urotensin I and sauvagine in vertebrates. CRH and urocortin act as anorexigenic factors for satiety regulation in rodents. In a goldfish model, intracerebroventricular (ICV) administration of ovine CRH (oCRH) affects not only food intake, but also locomotor activity. However, there is no information regarding the psychophysiological roles of CRH in goldfish. Therefore, we investigated the effect of oCRH on psychomotor activity in this species. ICV administration of synthetic oCRH at 20pmol/g body weight (BW) enhanced locomotor activity. Since intact goldfish prefer the lower to the upper area of a tank, we developed a method for measuring the time taken for fish to move from the lower to the upper area. ICV administration of oCRH at 20pmol/g BW and the central-type benzodiazepine receptor inverse agonist FG-7142 (an anxiogenic agent) at 1-4pmol/g BW both increased the time taken to move from the lower to the upper area. This anxiogenic-like effect of oCRH was abolished by the CRH receptor antagonist α-helical CRH(9-41) (100pmol/g BW). These results indicate that CRH can potently affect locomotor and psychomotor activities in goldfish.

AB - Corticotropin-releasing hormone (CRH) is a member of the hypothalamic neuropeptide family that includes urocortins, urotensin I and sauvagine in vertebrates. CRH and urocortin act as anorexigenic factors for satiety regulation in rodents. In a goldfish model, intracerebroventricular (ICV) administration of ovine CRH (oCRH) affects not only food intake, but also locomotor activity. However, there is no information regarding the psychophysiological roles of CRH in goldfish. Therefore, we investigated the effect of oCRH on psychomotor activity in this species. ICV administration of synthetic oCRH at 20pmol/g body weight (BW) enhanced locomotor activity. Since intact goldfish prefer the lower to the upper area of a tank, we developed a method for measuring the time taken for fish to move from the lower to the upper area. ICV administration of oCRH at 20pmol/g BW and the central-type benzodiazepine receptor inverse agonist FG-7142 (an anxiogenic agent) at 1-4pmol/g BW both increased the time taken to move from the lower to the upper area. This anxiogenic-like effect of oCRH was abolished by the CRH receptor antagonist α-helical CRH(9-41) (100pmol/g BW). These results indicate that CRH can potently affect locomotor and psychomotor activities in goldfish.

KW - Anxiogenic-like action

KW - CRH

KW - Goldfish

KW - ICV injection

KW - Locomotor activity

KW - Psychomotor activity

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U2 - 10.1016/j.ygcen.2013.01.001

DO - 10.1016/j.ygcen.2013.01.001

M3 - 学術論文

C2 - 23321398

AN - SCOPUS:84878959770

SN - 0016-6480

VL - 188

SP - 118

EP - 122

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

IS - 1

ER -

Ovine corticotropin-releasing hormone (oCRH) exerts an anxiogenic-like action in the goldfish, Carassius auratus

Abstract

Keywords

ASJC Scopus subject areas

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