Optical pH and oxygen sensing for micro-arrayed cell chips

Masayasu Suzuki, Hiroaki Nakabayashi, Yong Jing, Masaru Honda

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

13 Scopus citations

Abstract

We are developing micro-well array chip which has a quarter million wells on one chip, and diameter of each well is 10 μm and only one cell can be occupied for each well. In this paper, we prepared optical pH and oxygen sensors in each well for monitoring of cell activity. Fluorescein isothiocyanate (FITC) and Tris(1,10-phenanthroline)ruthenium (II) chloride (Ru complex) was used as pH and oxygen sensing indicators respectively. Polydimethylsiloxane (PDMS) film which has 10 μm diameter holes was put onto a slide glass. Cell activity could be successfully detected by using this optical pH and oxygen sensor array.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1482-1484
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 2005/10/092005/10/13

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period2005/10/092005/10/13

Keywords

  • Cell chip
  • Micro-array
  • Oxygen
  • PH

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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