Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes.

Yoshihiro Honma; Izumi Nakabayashi; Daisuke Tamaoki; Haruo Kasahara; Noriaki Ishioka; Toru Shimazu; Hirokazu Kasahara; Mitsuhiro Yamada; Ichirou Karahara; Seiichiro Kamisaka

doi:10.2187/bss.17.307

Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes.

Yoshihiro Honma, Izumi Nakabayashi, Daisuke Tamaoki, Haruo Kasahara, Noriaki Ishioka, Toru Shimazu, Hirokazu Kasahara, Mitsuhiro Yamada, Ichirou Karahara^*, Seiichiro Kamisaka

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Samples will be kept in non-fresh fixative for longer period than usual in Kennedy Space Center Fixation Tubes (KFT) when they will be fixed for microscopy in the International Space Station (ISS). It is necessary to examine characteristics of samples prepared under such conditions and to improve preservation of the samples. In this study, morphology of Arabidopsis tissues prepared under such conditions was examined under a light microscope. Deformation of cell shape was observed in tissues with well-developed intercellular spaces when they were fixed in non-fresh fixative for longer period in KFTs.

Original language	English
Pages (from-to)	307-308
Number of pages	2
Journal	Biological sciences in space = Uchū seibutsu kagaku
Volume	17
Issue number	4
DOIs	https://doi.org/10.2187/bss.17.307
State	Published - 2003/12

ASJC Scopus subject areas

General Medicine

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title = "Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes.",

abstract = "Samples will be kept in non-fresh fixative for longer period than usual in Kennedy Space Center Fixation Tubes (KFT) when they will be fixed for microscopy in the International Space Station (ISS). It is necessary to examine characteristics of samples prepared under such conditions and to improve preservation of the samples. In this study, morphology of Arabidopsis tissues prepared under such conditions was examined under a light microscope. Deformation of cell shape was observed in tissues with well-developed intercellular spaces when they were fixed in non-fresh fixative for longer period in KFTs.",

author = "Yoshihiro Honma and Izumi Nakabayashi and Daisuke Tamaoki and Haruo Kasahara and Noriaki Ishioka and Toru Shimazu and Hirokazu Kasahara and Mitsuhiro Yamada and Ichirou Karahara and Seiichiro Kamisaka",

year = "2003",

month = dec,

doi = "10.2187/bss.17.307",

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T1 - Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes.

AU - Honma, Yoshihiro

AU - Nakabayashi, Izumi

AU - Tamaoki, Daisuke

AU - Kasahara, Haruo

AU - Ishioka, Noriaki

AU - Shimazu, Toru

AU - Kasahara, Hirokazu

AU - Yamada, Mitsuhiro

AU - Karahara, Ichirou

AU - Kamisaka, Seiichiro

PY - 2003/12

Y1 - 2003/12

N2 - Samples will be kept in non-fresh fixative for longer period than usual in Kennedy Space Center Fixation Tubes (KFT) when they will be fixed for microscopy in the International Space Station (ISS). It is necessary to examine characteristics of samples prepared under such conditions and to improve preservation of the samples. In this study, morphology of Arabidopsis tissues prepared under such conditions was examined under a light microscope. Deformation of cell shape was observed in tissues with well-developed intercellular spaces when they were fixed in non-fresh fixative for longer period in KFTs.

AB - Samples will be kept in non-fresh fixative for longer period than usual in Kennedy Space Center Fixation Tubes (KFT) when they will be fixed for microscopy in the International Space Station (ISS). It is necessary to examine characteristics of samples prepared under such conditions and to improve preservation of the samples. In this study, morphology of Arabidopsis tissues prepared under such conditions was examined under a light microscope. Deformation of cell shape was observed in tissues with well-developed intercellular spaces when they were fixed in non-fresh fixative for longer period in KFTs.

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JO - Biological sciences in space = Uchū seibutsu kagaku

JF - Biological sciences in space = Uchū seibutsu kagaku

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ER -

Optical microscopy of Arabidopsis seedlings fixed in non-fresh FAA using Kennedy Fixation Tubes.

Abstract

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