Nonlamellar Liquid Crystalline Phases and Their Particle Formation in the Egg Yolk Phosphatidylcholine/Diolein System

Tomoari Kamo; Minoru Nakano; Warunee Leesajakul; Atsuhiko Sugita; Hideki Matsuoka; Tetsurou Handa

doi:10.1021/la035313x

Nonlamellar Liquid Crystalline Phases and Their Particle Formation in the Egg Yolk Phosphatidylcholine/Diolein System

Tomoari Kamo, Minoru Nakano^*, Warunee Leesajakul, Atsuhiko Sugita, Hideki Matsuoka, Tetsurou Handa

^*Corresponding author for this work

Biointerface Chemistry

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The phase behavior of fully hydrated egg yolk phosphatidylcholine (EPC)/diolein (DO) mixtures was investigated by small-angle X-ray scattering and ³¹P NMR. EPC formed a lamellar phase, whereas DO gave a negative curvature to the lipid membranes, leading to nonlamellar phase formation. At an EPC/DO molar ratio of 8:2, the mixture formed a bicontinuous cubic phase of primitive type. A bicontinuous cubic phase of diamond type was also observed at the ratio of 7:3, but it coexisted with an inverted hexagonal (H_II) phase. The mixture with higher DO fractions formed the H_II phase. We further investigated if these nonlamellar phases could be dispersed by high-pressure emulsification with Pluronic F127, to form cubosomes or hexosomes, as have been obtained in monoolein-based cubic and H_II phases (Langmuir 2001, 17, 3917-3922; Langmuir 2002, 18, 9283-9288). As a result, cubosomes could not be obtained and vesicles were formed instead, presumably due to the existence of F127, which gave a positive curvature to the negatively curved membranes. However, the H_II phase, which was less compatible with F127, could be dispersed to form hexosomes.

Original language	English
Pages (from-to)	9191-9195
Number of pages	5
Journal	Langmuir
Volume	19
Issue number	22
DOIs	https://doi.org/10.1021/la035313x
State	Published - 2003/10/28

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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title = "Nonlamellar Liquid Crystalline Phases and Their Particle Formation in the Egg Yolk Phosphatidylcholine/Diolein System",

abstract = "The phase behavior of fully hydrated egg yolk phosphatidylcholine (EPC)/diolein (DO) mixtures was investigated by small-angle X-ray scattering and 31P NMR. EPC formed a lamellar phase, whereas DO gave a negative curvature to the lipid membranes, leading to nonlamellar phase formation. At an EPC/DO molar ratio of 8:2, the mixture formed a bicontinuous cubic phase of primitive type. A bicontinuous cubic phase of diamond type was also observed at the ratio of 7:3, but it coexisted with an inverted hexagonal (HII) phase. The mixture with higher DO fractions formed the HII phase. We further investigated if these nonlamellar phases could be dispersed by high-pressure emulsification with Pluronic F127, to form cubosomes or hexosomes, as have been obtained in monoolein-based cubic and HII phases (Langmuir 2001, 17, 3917-3922; Langmuir 2002, 18, 9283-9288). As a result, cubosomes could not be obtained and vesicles were formed instead, presumably due to the existence of F127, which gave a positive curvature to the negatively curved membranes. However, the HII phase, which was less compatible with F127, could be dispersed to form hexosomes.",

author = "Tomoari Kamo and Minoru Nakano and Warunee Leesajakul and Atsuhiko Sugita and Hideki Matsuoka and Tetsurou Handa",

year = "2003",

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doi = "10.1021/la035313x",

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T1 - Nonlamellar Liquid Crystalline Phases and Their Particle Formation in the Egg Yolk Phosphatidylcholine/Diolein System

AU - Kamo, Tomoari

AU - Nakano, Minoru

AU - Leesajakul, Warunee

AU - Sugita, Atsuhiko

AU - Matsuoka, Hideki

AU - Handa, Tetsurou

PY - 2003/10/28

Y1 - 2003/10/28

N2 - The phase behavior of fully hydrated egg yolk phosphatidylcholine (EPC)/diolein (DO) mixtures was investigated by small-angle X-ray scattering and 31P NMR. EPC formed a lamellar phase, whereas DO gave a negative curvature to the lipid membranes, leading to nonlamellar phase formation. At an EPC/DO molar ratio of 8:2, the mixture formed a bicontinuous cubic phase of primitive type. A bicontinuous cubic phase of diamond type was also observed at the ratio of 7:3, but it coexisted with an inverted hexagonal (HII) phase. The mixture with higher DO fractions formed the HII phase. We further investigated if these nonlamellar phases could be dispersed by high-pressure emulsification with Pluronic F127, to form cubosomes or hexosomes, as have been obtained in monoolein-based cubic and HII phases (Langmuir 2001, 17, 3917-3922; Langmuir 2002, 18, 9283-9288). As a result, cubosomes could not be obtained and vesicles were formed instead, presumably due to the existence of F127, which gave a positive curvature to the negatively curved membranes. However, the HII phase, which was less compatible with F127, could be dispersed to form hexosomes.

AB - The phase behavior of fully hydrated egg yolk phosphatidylcholine (EPC)/diolein (DO) mixtures was investigated by small-angle X-ray scattering and 31P NMR. EPC formed a lamellar phase, whereas DO gave a negative curvature to the lipid membranes, leading to nonlamellar phase formation. At an EPC/DO molar ratio of 8:2, the mixture formed a bicontinuous cubic phase of primitive type. A bicontinuous cubic phase of diamond type was also observed at the ratio of 7:3, but it coexisted with an inverted hexagonal (HII) phase. The mixture with higher DO fractions formed the HII phase. We further investigated if these nonlamellar phases could be dispersed by high-pressure emulsification with Pluronic F127, to form cubosomes or hexosomes, as have been obtained in monoolein-based cubic and HII phases (Langmuir 2001, 17, 3917-3922; Langmuir 2002, 18, 9283-9288). As a result, cubosomes could not be obtained and vesicles were formed instead, presumably due to the existence of F127, which gave a positive curvature to the negatively curved membranes. However, the HII phase, which was less compatible with F127, could be dispersed to form hexosomes.

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Nonlamellar Liquid Crystalline Phases and Their Particle Formation in the Egg Yolk Phosphatidylcholine/Diolein System

Abstract

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