Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11

Masakado Matsumoto; Kenji Sakae; Michio Ohta; Miyoko Endo; Rumi Okuno; Shoko Murayama; Kyoko Hirasawa; Rieko Suzuki; Junko Isobe; Daisuke Tanaka; Chihiro Katsukawa; Aki Tamaru; Masaaki Tomita; Kikuyo Ogata; Tomihisa Yasuoka; Tadayoshi Ikebe; Haruo Watanabe

doi:10.1016/j.ijantimicag.2004.09.014

Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11

Masakado Matsumoto^*, Kenji Sakae, Michio Ohta, Miyoko Endo, Rumi Okuno, Shoko Murayama, Kyoko Hirasawa, Rieko Suzuki, Junko Isobe, Daisuke Tanaka, Chihiro Katsukawa, Aki Tamaru, Masaaki Tomita, Kikuyo Ogata, Tomihisa Yasuoka, Tadayoshi Ikebe, Haruo Watanabe

^*Corresponding author for this work

Department of Natural and Environmental Science

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The molecular mechanism of high level tetracycline resistance in T serotypes 4 and 11 group A streptococcal (GAS) isolates was examined in 61 tetracycline-resistant isolates in Japan. PCR and sequencing analyses revealed that the T serotype/emm genotype, T4/4 isolates carried tet(O) genes, which were genetically homogenous. The T11/11 and T11/89 isolates carried different subtypes of tet(M) genes, which were present on transposons Tn916 and Tn1545, respectively. In addition, these T11 isolates may have obtained the tet(M) gene after the 1990s, because resistance to tetracycline in T11 isolates was rarely found before then. These results strongly suggested that the T4 and T11 GAS isolates acquired tetracycline-resistance via different molecular mechanisms.

Original language	English
Pages (from-to)	142-147
Number of pages	6
Journal	International Journal of Antimicrobial Agents
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/j.ijantimicag.2004.09.014
State	Published - 2005/02

Keywords

Group A Streptococcus (GAS)
Tetracycline
tet gene

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

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10.1016/j.ijantimicag.2004.09.014

Cite this

Matsumoto, M., Sakae, K., Ohta, M., Endo, M., Okuno, R., Murayama, S., Hirasawa, K., Suzuki, R., Isobe, J., Tanaka, D., Katsukawa, C., Tamaru, A., Tomita, M., Ogata, K., Yasuoka, T., Ikebe, T., & Watanabe, H. (2005). Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11. International Journal of Antimicrobial Agents, 25(2), 142-147. https://doi.org/10.1016/j.ijantimicag.2004.09.014

@article{27c3387b0429486db46453d16f30091e,

title = "Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11",

abstract = "The molecular mechanism of high level tetracycline resistance in T serotypes 4 and 11 group A streptococcal (GAS) isolates was examined in 61 tetracycline-resistant isolates in Japan. PCR and sequencing analyses revealed that the T serotype/emm genotype, T4/4 isolates carried tet(O) genes, which were genetically homogenous. The T11/11 and T11/89 isolates carried different subtypes of tet(M) genes, which were present on transposons Tn916 and Tn1545, respectively. In addition, these T11 isolates may have obtained the tet(M) gene after the 1990s, because resistance to tetracycline in T11 isolates was rarely found before then. These results strongly suggested that the T4 and T11 GAS isolates acquired tetracycline-resistance via different molecular mechanisms.",

keywords = "Group A Streptococcus (GAS), Tetracycline, tet gene",

author = "Masakado Matsumoto and Kenji Sakae and Michio Ohta and Miyoko Endo and Rumi Okuno and Shoko Murayama and Kyoko Hirasawa and Rieko Suzuki and Junko Isobe and Daisuke Tanaka and Chihiro Katsukawa and Aki Tamaru and Masaaki Tomita and Kikuyo Ogata and Tomihisa Yasuoka and Tadayoshi Ikebe and Haruo Watanabe",

year = "2005",

month = feb,

doi = "10.1016/j.ijantimicag.2004.09.014",

language = "英語",

volume = "25",

pages = "142--147",

journal = "International Journal of Antimicrobial Agents",

issn = "0924-8579",

publisher = "Elsevier B.V.",

number = "2",

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Matsumoto, M, Sakae, K, Ohta, M, Endo, M, Okuno, R, Murayama, S, Hirasawa, K, Suzuki, R, Isobe, J, Tanaka, D, Katsukawa, C, Tamaru, A, Tomita, M, Ogata, K, Yasuoka, T, Ikebe, T & Watanabe, H 2005, 'Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11', International Journal of Antimicrobial Agents, vol. 25, no. 2, pp. 142-147. https://doi.org/10.1016/j.ijantimicag.2004.09.014

TY - JOUR

T1 - Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11

AU - Matsumoto, Masakado

AU - Sakae, Kenji

AU - Ohta, Michio

AU - Endo, Miyoko

AU - Okuno, Rumi

AU - Murayama, Shoko

AU - Hirasawa, Kyoko

AU - Suzuki, Rieko

AU - Isobe, Junko

AU - Tanaka, Daisuke

AU - Katsukawa, Chihiro

AU - Tamaru, Aki

AU - Tomita, Masaaki

AU - Ogata, Kikuyo

AU - Yasuoka, Tomihisa

AU - Ikebe, Tadayoshi

AU - Watanabe, Haruo

PY - 2005/2

Y1 - 2005/2

N2 - The molecular mechanism of high level tetracycline resistance in T serotypes 4 and 11 group A streptococcal (GAS) isolates was examined in 61 tetracycline-resistant isolates in Japan. PCR and sequencing analyses revealed that the T serotype/emm genotype, T4/4 isolates carried tet(O) genes, which were genetically homogenous. The T11/11 and T11/89 isolates carried different subtypes of tet(M) genes, which were present on transposons Tn916 and Tn1545, respectively. In addition, these T11 isolates may have obtained the tet(M) gene after the 1990s, because resistance to tetracycline in T11 isolates was rarely found before then. These results strongly suggested that the T4 and T11 GAS isolates acquired tetracycline-resistance via different molecular mechanisms.

AB - The molecular mechanism of high level tetracycline resistance in T serotypes 4 and 11 group A streptococcal (GAS) isolates was examined in 61 tetracycline-resistant isolates in Japan. PCR and sequencing analyses revealed that the T serotype/emm genotype, T4/4 isolates carried tet(O) genes, which were genetically homogenous. The T11/11 and T11/89 isolates carried different subtypes of tet(M) genes, which were present on transposons Tn916 and Tn1545, respectively. In addition, these T11 isolates may have obtained the tet(M) gene after the 1990s, because resistance to tetracycline in T11 isolates was rarely found before then. These results strongly suggested that the T4 and T11 GAS isolates acquired tetracycline-resistance via different molecular mechanisms.

KW - Group A Streptococcus (GAS)

KW - Tetracycline

KW - tet gene

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U2 - 10.1016/j.ijantimicag.2004.09.014

DO - 10.1016/j.ijantimicag.2004.09.014

M3 - 学術論文

C2 - 15664484

AN - SCOPUS:19944433863

SN - 0924-8579

VL - 25

SP - 142

EP - 147

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

IS - 2

ER -

Molecular mechanisms of high level tetracycline-resistance in group A streptococcal isolates, T serotypes 4 and 11

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Keywords

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