Molecular film technology for biosensors

Masuo Aizawa*, Koji Owaku, Mieko Matsuzawa, Hiroaki Shinohara, Yoshihito Ikariyama

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Two different molecular film technologies have been developed for fabricating biosensors. Firstly, bovine serum albumin spread on the air-water interface was cross-linked and transferred onto the glass surface, on which various molecular assemblies were formed by the bioaffinity technique. Secondly, a protein monolayer was adsorbed from an aqueous phase on the potential-controlled electrode surface, which was followed by electrochemical polymerization and deposition of pyrrole between the protein molecules to make the adsorbed protein film conductive. A unique optical biosensor for taste has been fabricated by depositing fluorophore-containing Langmuir-Blodgett (LB) films on the glass surface. Each layer of the LB films exhibits its characteristic fluorescence peak which is quenched or enhanced in contact with taste substances. The sensor consisted of three different LB films containing pyrene and anthracene and deposited on a glass plate. Novel performance was demonstrated with the response of the sensor to glutamate and nucleotide umami substances.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalThin Solid Films
Volume180
Issue number1-2
DOIs
StatePublished - 1989/11/21

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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