TY - JOUR
T1 - Modulation of apoptosis and epithelial-mesenchymal transition e-cadherin/TGF-β/Snail/TWIST pathways by a new ciprofloxacin chalcone in breast cancer cells
AU - Alaaeldin, Rania
AU - Abuo-Rahma, Gamal El Din A.
AU - Zhao, Qing Li
AU - Fathy, Moustafa
N1 - Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.
PY - 2021/5
Y1 - 2021/5
N2 - Background/Aim: This study aimed to investigate the effect of the new ciprofloxacin chalcone [7-(4-(Nsubstituted carbamoyl methyl) piperazin-1 yl)] on the proliferation, migration, and metastasis of MCF-7 and MDAMB-231 breast cancer cell lines. Materials and Methods: Cell viability, colony formation and cell migration abilities were analysed. Cell cycle distribution and apoptosis were examined by flow cytometry. The molecular mechanism underlying chalcone s activity was investigated using qRT-PCR and western blotting. Results: This new ciprofloxacin chalcone significantly inhibited proliferation, colony formation, and cell migration abilities of both cancer cell lines. Furthermore, it initiated apoptosis and caused cell cycle arrest at G2/M and S phase in MCF-7 and MDA-MB-231 cell lines, respectively. In addition, it up-regulated the expression of pro-Apoptotic factors, p53, PUMA and NOXA, and down-regulated the expression of anti-Apoptotic factors, MDM2 and MDM4. At the same time, it inhibited epithelial mesenchymal transition by increasing the expression of E-cadherin and decreasing the expression of TGF-β1, SNAI1, TWIST1, MMP2, and MMP9. Conclusion: This new ciprofloxacin chalcone exhibited promising apoptotic and anti-metastatic activities against MCF-7 and MDA-MB-231 breast cancer cell lines, and, therefore, is an attractive molecule for drug development in the treatment of breast cancer. Therefore Is an attractive molecule for drug development in the treatment of breast cancer.
AB - Background/Aim: This study aimed to investigate the effect of the new ciprofloxacin chalcone [7-(4-(Nsubstituted carbamoyl methyl) piperazin-1 yl)] on the proliferation, migration, and metastasis of MCF-7 and MDAMB-231 breast cancer cell lines. Materials and Methods: Cell viability, colony formation and cell migration abilities were analysed. Cell cycle distribution and apoptosis were examined by flow cytometry. The molecular mechanism underlying chalcone s activity was investigated using qRT-PCR and western blotting. Results: This new ciprofloxacin chalcone significantly inhibited proliferation, colony formation, and cell migration abilities of both cancer cell lines. Furthermore, it initiated apoptosis and caused cell cycle arrest at G2/M and S phase in MCF-7 and MDA-MB-231 cell lines, respectively. In addition, it up-regulated the expression of pro-Apoptotic factors, p53, PUMA and NOXA, and down-regulated the expression of anti-Apoptotic factors, MDM2 and MDM4. At the same time, it inhibited epithelial mesenchymal transition by increasing the expression of E-cadherin and decreasing the expression of TGF-β1, SNAI1, TWIST1, MMP2, and MMP9. Conclusion: This new ciprofloxacin chalcone exhibited promising apoptotic and anti-metastatic activities against MCF-7 and MDA-MB-231 breast cancer cell lines, and, therefore, is an attractive molecule for drug development in the treatment of breast cancer. Therefore Is an attractive molecule for drug development in the treatment of breast cancer.
KW - Apoptosis
KW - Breast cancer
KW - Ciprofloxacin chalcone
KW - E-cadherin
KW - Epithelial mesenchymal transition
KW - Metastasis
KW - P53
KW - TGF-β
UR - http://www.scopus.com/inward/record.url?scp=85105443839&partnerID=8YFLogxK
U2 - 10.21873/ANTICANRES.15013
DO - 10.21873/ANTICANRES.15013
M3 - 学術論文
C2 - 33952463
AN - SCOPUS:85105443839
SN - 0250-7005
VL - 41
JO - Anticancer Research
JF - Anticancer Research
IS - 5
ER -