Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma

Michiyo Hayakawa; J. Nicholas Taylor; Ryuta Nakao; Kentaro Mochizuki; Yuki Sawai; Kosuke Hashimoto; Koji Tabata; Yasuaki Kumamoto; Katsumasa Fujita; Eiichi Konishi; Shigeru Hirano; Hideo Tanaka; Tamiki Komatsuzaki; Yoshinori Harada

doi:10.1016/j.bbrc.2022.12.007

Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma

Michiyo Hayakawa, J. Nicholas Taylor, Ryuta Nakao, Kentaro Mochizuki, Yuki Sawai, Kosuke Hashimoto, Koji Tabata, Yasuaki Kumamoto, Katsumasa Fujita, Eiichi Konishi, Shigeru Hirano, Hideo Tanaka, Tamiki Komatsuzaki^*, Yoshinori Harada^*

^*Corresponding author for this work

Research Center for Pre-Disease Science

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Follicular neoplasms of the thyroid include follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA). However, the differences in cytological findings between FTC and FTA remain undetermined. Here, we aimed to evaluate the accumulation of lipid droplets (LDs) and the expression of adipophilin (perilipin 2/ADRP/ADFP), a known LD marker, in cultured FTC cells. We also immunohistochemically compared adipophilin expression in the FTC and FTA of resected human thyroid tissues. Cultured FTC (FTC-133 and RO82W-1) possessed increased populations of LDs compared to thyroid follicular epithelial (Nthy-ori 3-1) cells. In vitro treatment with phosphatidylinositol-3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling inhibitors (LY294002, MK2206, and rapamycin) in FTC-133 cells downregulated the PI3K/Akt/mTOR/sterol regulatory element-binding protein 1 (SREBP1) signaling pathway, resulting in a significant reduction in LD accumulation. SREBP1 is a master transcription factor that controls lipid metabolism. Fluorescence immunocytochemistry revealed adipophilin expression in the LDs of FTC-133 cells. Immunohistochemical analysis of surgically resected human thyroid tissues revealed significantly increased expression of adipophilin in FTC compared with FTA and adjacent non-tumorous thyroid epithelia. Taken together, LDs and adipophilin were abundant in cultured FTC; the evaluation of adipophilin expression can help distinguish FTC from FTA in surgical specimens.

Original language	English
Pages (from-to)	192-201
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	640
DOIs	https://doi.org/10.1016/j.bbrc.2022.12.007
State	Published - 2023/01/15

Keywords

Adipophilin
Follicular neoplasm
Follicular thyroid adenoma
Follicular thyroid carcinoma
Lipid droplet

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Hayakawa, M., Taylor, J. N., Nakao, R., Mochizuki, K., Sawai, Y., Hashimoto, K., Tabata, K., Kumamoto, Y., Fujita, K., Konishi, E., Hirano, S., Tanaka, H., Komatsuzaki, T., & Harada, Y. (2023). Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma. Biochemical and Biophysical Research Communications, 640, 192-201. https://doi.org/10.1016/j.bbrc.2022.12.007

@article{1064c2c8e7b04097b37fa35b8cee6599,

title = "Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma",

abstract = "Follicular neoplasms of the thyroid include follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA). However, the differences in cytological findings between FTC and FTA remain undetermined. Here, we aimed to evaluate the accumulation of lipid droplets (LDs) and the expression of adipophilin (perilipin 2/ADRP/ADFP), a known LD marker, in cultured FTC cells. We also immunohistochemically compared adipophilin expression in the FTC and FTA of resected human thyroid tissues. Cultured FTC (FTC-133 and RO82W-1) possessed increased populations of LDs compared to thyroid follicular epithelial (Nthy-ori 3-1) cells. In vitro treatment with phosphatidylinositol-3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling inhibitors (LY294002, MK2206, and rapamycin) in FTC-133 cells downregulated the PI3K/Akt/mTOR/sterol regulatory element-binding protein 1 (SREBP1) signaling pathway, resulting in a significant reduction in LD accumulation. SREBP1 is a master transcription factor that controls lipid metabolism. Fluorescence immunocytochemistry revealed adipophilin expression in the LDs of FTC-133 cells. Immunohistochemical analysis of surgically resected human thyroid tissues revealed significantly increased expression of adipophilin in FTC compared with FTA and adjacent non-tumorous thyroid epithelia. Taken together, LDs and adipophilin were abundant in cultured FTC; the evaluation of adipophilin expression can help distinguish FTC from FTA in surgical specimens.",

keywords = "Adipophilin, Follicular neoplasm, Follicular thyroid adenoma, Follicular thyroid carcinoma, Lipid droplet",

author = "Michiyo Hayakawa and Taylor, {J. Nicholas} and Ryuta Nakao and Kentaro Mochizuki and Yuki Sawai and Kosuke Hashimoto and Koji Tabata and Yasuaki Kumamoto and Katsumasa Fujita and Eiichi Konishi and Shigeru Hirano and Hideo Tanaka and Tamiki Komatsuzaki and Yoshinori Harada",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2023",

month = jan,

day = "15",

doi = "10.1016/j.bbrc.2022.12.007",

language = "英語",

volume = "640",

pages = "192--201",

journal = "Biochemical and Biophysical Research Communications",

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Hayakawa, M, Taylor, JN, Nakao, R, Mochizuki, K, Sawai, Y, Hashimoto, K, Tabata, K, Kumamoto, Y, Fujita, K, Konishi, E, Hirano, S, Tanaka, H, Komatsuzaki, T & Harada, Y 2023, 'Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma', Biochemical and Biophysical Research Communications, vol. 640, pp. 192-201. https://doi.org/10.1016/j.bbrc.2022.12.007

TY - JOUR

T1 - Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma

AU - Hayakawa, Michiyo

AU - Taylor, J. Nicholas

AU - Nakao, Ryuta

AU - Mochizuki, Kentaro

AU - Sawai, Yuki

AU - Hashimoto, Kosuke

AU - Tabata, Koji

AU - Kumamoto, Yasuaki

AU - Fujita, Katsumasa

AU - Konishi, Eiichi

AU - Hirano, Shigeru

AU - Tanaka, Hideo

AU - Komatsuzaki, Tamiki

AU - Harada, Yoshinori

PY - 2023/1/15

Y1 - 2023/1/15

N2 - Follicular neoplasms of the thyroid include follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA). However, the differences in cytological findings between FTC and FTA remain undetermined. Here, we aimed to evaluate the accumulation of lipid droplets (LDs) and the expression of adipophilin (perilipin 2/ADRP/ADFP), a known LD marker, in cultured FTC cells. We also immunohistochemically compared adipophilin expression in the FTC and FTA of resected human thyroid tissues. Cultured FTC (FTC-133 and RO82W-1) possessed increased populations of LDs compared to thyroid follicular epithelial (Nthy-ori 3-1) cells. In vitro treatment with phosphatidylinositol-3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling inhibitors (LY294002, MK2206, and rapamycin) in FTC-133 cells downregulated the PI3K/Akt/mTOR/sterol regulatory element-binding protein 1 (SREBP1) signaling pathway, resulting in a significant reduction in LD accumulation. SREBP1 is a master transcription factor that controls lipid metabolism. Fluorescence immunocytochemistry revealed adipophilin expression in the LDs of FTC-133 cells. Immunohistochemical analysis of surgically resected human thyroid tissues revealed significantly increased expression of adipophilin in FTC compared with FTA and adjacent non-tumorous thyroid epithelia. Taken together, LDs and adipophilin were abundant in cultured FTC; the evaluation of adipophilin expression can help distinguish FTC from FTA in surgical specimens.

AB - Follicular neoplasms of the thyroid include follicular thyroid carcinoma (FTC) and follicular thyroid adenoma (FTA). However, the differences in cytological findings between FTC and FTA remain undetermined. Here, we aimed to evaluate the accumulation of lipid droplets (LDs) and the expression of adipophilin (perilipin 2/ADRP/ADFP), a known LD marker, in cultured FTC cells. We also immunohistochemically compared adipophilin expression in the FTC and FTA of resected human thyroid tissues. Cultured FTC (FTC-133 and RO82W-1) possessed increased populations of LDs compared to thyroid follicular epithelial (Nthy-ori 3-1) cells. In vitro treatment with phosphatidylinositol-3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling inhibitors (LY294002, MK2206, and rapamycin) in FTC-133 cells downregulated the PI3K/Akt/mTOR/sterol regulatory element-binding protein 1 (SREBP1) signaling pathway, resulting in a significant reduction in LD accumulation. SREBP1 is a master transcription factor that controls lipid metabolism. Fluorescence immunocytochemistry revealed adipophilin expression in the LDs of FTC-133 cells. Immunohistochemical analysis of surgically resected human thyroid tissues revealed significantly increased expression of adipophilin in FTC compared with FTA and adjacent non-tumorous thyroid epithelia. Taken together, LDs and adipophilin were abundant in cultured FTC; the evaluation of adipophilin expression can help distinguish FTC from FTA in surgical specimens.

KW - Adipophilin

KW - Follicular neoplasm

KW - Follicular thyroid adenoma

KW - Follicular thyroid carcinoma

KW - Lipid droplet

UR - http://www.scopus.com/inward/record.url?scp=85143964311&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2022.12.007

DO - 10.1016/j.bbrc.2022.12.007

M3 - 学術論文

C2 - 36521425

AN - SCOPUS:85143964311

SN - 0006-291X

VL - 640

SP - 192

EP - 201

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

ER -

Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma

Abstract

Keywords

ASJC Scopus subject areas

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