Isotype-specific changes in the amount of β-tubulin RNA in synchronized tobacco BY2 cells

Shoji Okamura; Koichi Okahara; Takayuki Iida; Miwa Ozaki; Sayaka Asano; Masashi Morita; Tsuneo Imanaka

doi:10.1247/csf.24.117

Isotype-specific changes in the amount of β-tubulin RNA in synchronized tobacco BY2 cells

Shoji Okamura^*, Koichi Okahara, Takayuki Iida, Miwa Ozaki, Sayaka Asano, Masashi Morita, Tsuneo Imanaka

^*Corresponding author for this work

Molecular Cell Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The 3'-ends of the β-tubulin cDNA were amplified from tobacco BY2 polyA⁺ RNA. According to the differences in the predicted amino acid sequence at the extreme C-terminal, they were grouped into three different isotypes, NTB1 in which 'EEGDYYEEDEEDLNEA', NTB2 in which 'EEEYYEDEEEAQED' and NTB3 in which 'DECEYEEEEEYDHEGN' follows the conservative 'YQQYQDATAD' sequence. Using unique 3'-untranslated regions as probes, changes in the RNA levels of each β-tubulin isotype were determined by dot-blot hybridization. The levels exhibited characteristic rhythms in the cell cycle. NTB1 RNA was highest in S phase in comparison to NTB2 RNA level which was highest in late G2. On the other hand, NTB3 RNA level was highest in early G2.

Original language	English
Pages (from-to)	117-122
Number of pages	6
Journal	Cell Structure and Function
Volume	24
Issue number	3
DOIs	https://doi.org/10.1247/csf.24.117
State	Published - 1999

Keywords

Cell cycle
RNA level
Tobacco BY2
β-tubulin isotypes

ASJC Scopus subject areas

Physiology
Molecular Biology
Cell Biology

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10.1247/csf.24.117

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title = "Isotype-specific changes in the amount of β-tubulin RNA in synchronized tobacco BY2 cells",

abstract = "The 3'-ends of the β-tubulin cDNA were amplified from tobacco BY2 polyA+ RNA. According to the differences in the predicted amino acid sequence at the extreme C-terminal, they were grouped into three different isotypes, NTB1 in which 'EEGDYYEEDEEDLNEA', NTB2 in which 'EEEYYEDEEEAQED' and NTB3 in which 'DECEYEEEEEYDHEGN' follows the conservative 'YQQYQDATAD' sequence. Using unique 3'-untranslated regions as probes, changes in the RNA levels of each β-tubulin isotype were determined by dot-blot hybridization. The levels exhibited characteristic rhythms in the cell cycle. NTB1 RNA was highest in S phase in comparison to NTB2 RNA level which was highest in late G2. On the other hand, NTB3 RNA level was highest in early G2.",

keywords = "Cell cycle, RNA level, Tobacco BY2, β-tubulin isotypes",

author = "Shoji Okamura and Koichi Okahara and Takayuki Iida and Miwa Ozaki and Sayaka Asano and Masashi Morita and Tsuneo Imanaka",

year = "1999",

doi = "10.1247/csf.24.117",

language = "英語",

volume = "24",

pages = "117--122",

journal = "Cell Structure and Function",

issn = "0386-7196",

publisher = "Japan Society for Cell Biology",

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T1 - Isotype-specific changes in the amount of β-tubulin RNA in synchronized tobacco BY2 cells

AU - Okamura, Shoji

AU - Okahara, Koichi

AU - Iida, Takayuki

AU - Ozaki, Miwa

AU - Asano, Sayaka

AU - Morita, Masashi

AU - Imanaka, Tsuneo

PY - 1999

Y1 - 1999

N2 - The 3'-ends of the β-tubulin cDNA were amplified from tobacco BY2 polyA+ RNA. According to the differences in the predicted amino acid sequence at the extreme C-terminal, they were grouped into three different isotypes, NTB1 in which 'EEGDYYEEDEEDLNEA', NTB2 in which 'EEEYYEDEEEAQED' and NTB3 in which 'DECEYEEEEEYDHEGN' follows the conservative 'YQQYQDATAD' sequence. Using unique 3'-untranslated regions as probes, changes in the RNA levels of each β-tubulin isotype were determined by dot-blot hybridization. The levels exhibited characteristic rhythms in the cell cycle. NTB1 RNA was highest in S phase in comparison to NTB2 RNA level which was highest in late G2. On the other hand, NTB3 RNA level was highest in early G2.

AB - The 3'-ends of the β-tubulin cDNA were amplified from tobacco BY2 polyA+ RNA. According to the differences in the predicted amino acid sequence at the extreme C-terminal, they were grouped into three different isotypes, NTB1 in which 'EEGDYYEEDEEDLNEA', NTB2 in which 'EEEYYEDEEEAQED' and NTB3 in which 'DECEYEEEEEYDHEGN' follows the conservative 'YQQYQDATAD' sequence. Using unique 3'-untranslated regions as probes, changes in the RNA levels of each β-tubulin isotype were determined by dot-blot hybridization. The levels exhibited characteristic rhythms in the cell cycle. NTB1 RNA was highest in S phase in comparison to NTB2 RNA level which was highest in late G2. On the other hand, NTB3 RNA level was highest in early G2.

KW - Cell cycle

KW - RNA level

KW - Tobacco BY2

KW - β-tubulin isotypes

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DO - 10.1247/csf.24.117

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ER -

Isotype-specific changes in the amount of β-tubulin RNA in synchronized tobacco BY2 cells

Abstract

Keywords

ASJC Scopus subject areas

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