Isolation and characterization of 25 polymorphic microsatellites of the large Japanese wood mouse (Apodemus speciosus)

Noriko Azuma; Tsukasa Okano; Masanori Tamaoki; Nobuyoshi Nakajima; Noriko Takamura; Yasushi Yokohata; Junji Shindo; Manabu Onuma

doi:10.1007/s12686-013-9953-1

Isolation and characterization of 25 polymorphic microsatellites of the large Japanese wood mouse (Apodemus speciosus)

Noriko Azuma, Tsukasa Okano, Masanori Tamaoki, Nobuyoshi Nakajima, Noriko Takamura, Yasushi Yokohata, Junji Shindo, Manabu Onuma^*

^*Corresponding author for this work

Department of Natural and Environmental Science

Research output: Contribution to journal › Article › peer-review

Abstract

The large Japanese wood mouse (Apodemus speciosus) is common, but endemic to Japan, and its population structure was affected by habitat fragmentation because of urbanization. It suggested that the species might be one of the important models for the conservation of ecosystems and biodiversity affected by humans, including the effect of radioactive discharge caused by nuclear power plant accidents at Fukushima. We developed and characterized 25 novel polymorphic microsatellite markers from the next-generation sequencing data in an effort to provide an effective tool for genetic studies on this species. In 8 individuals from Aomori, Japan, the number of alleles and expected heterozygosities ranged from 5 to 13 and from 0.795 to 0.991, respectively, suggesting the availability of these markers for genetic studies in this species.

Original language	English
Pages (from-to)	1001-1003
Number of pages	3
Journal	Conservation Genetics Resources
Volume	5
Issue number	4
DOIs	https://doi.org/10.1007/s12686-013-9953-1
State	Published - 2013/12

Keywords

Apodemus speciosus
Microsatellite
NGS
Wood mouse

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-013-9953-1

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title = "Isolation and characterization of 25 polymorphic microsatellites of the large Japanese wood mouse (Apodemus speciosus)",

abstract = "The large Japanese wood mouse (Apodemus speciosus) is common, but endemic to Japan, and its population structure was affected by habitat fragmentation because of urbanization. It suggested that the species might be one of the important models for the conservation of ecosystems and biodiversity affected by humans, including the effect of radioactive discharge caused by nuclear power plant accidents at Fukushima. We developed and characterized 25 novel polymorphic microsatellite markers from the next-generation sequencing data in an effort to provide an effective tool for genetic studies on this species. In 8 individuals from Aomori, Japan, the number of alleles and expected heterozygosities ranged from 5 to 13 and from 0.795 to 0.991, respectively, suggesting the availability of these markers for genetic studies in this species.",

keywords = "Apodemus speciosus, Microsatellite, NGS, Wood mouse",

author = "Noriko Azuma and Tsukasa Okano and Masanori Tamaoki and Nobuyoshi Nakajima and Noriko Takamura and Yasushi Yokohata and Junji Shindo and Manabu Onuma",

note = "Funding Information: Acknowledgments The authors wish to thank Akira Yasuda, Hir-oaki Ishida, Akitsu Miyamoto, and Shiho Arai of the Laboratory of Wildlife Conservation, Graduate School of Science and Engineering, University of Toyama for their valuable assistance in animal capture. The present study was financially supported by the project {\textquoteleft}{\textquoteleft}Study on dynamics of radioactive materials in multimedia environment{\textquoteright}{\textquoteright} commissioned by the Ministry of the Environment Japan.",

year = "2013",

month = dec,

doi = "10.1007/s12686-013-9953-1",

language = "英語",

volume = "5",

pages = "1001--1003",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer Science and Business Media B.V.",

number = "4",

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TY - JOUR

T1 - Isolation and characterization of 25 polymorphic microsatellites of the large Japanese wood mouse (Apodemus speciosus)

AU - Azuma, Noriko

AU - Okano, Tsukasa

AU - Tamaoki, Masanori

AU - Nakajima, Nobuyoshi

AU - Takamura, Noriko

AU - Yokohata, Yasushi

AU - Shindo, Junji

AU - Onuma, Manabu

N1 - Funding Information: Acknowledgments The authors wish to thank Akira Yasuda, Hir-oaki Ishida, Akitsu Miyamoto, and Shiho Arai of the Laboratory of Wildlife Conservation, Graduate School of Science and Engineering, University of Toyama for their valuable assistance in animal capture. The present study was financially supported by the project ‘‘Study on dynamics of radioactive materials in multimedia environment’’ commissioned by the Ministry of the Environment Japan.

PY - 2013/12

Y1 - 2013/12

N2 - The large Japanese wood mouse (Apodemus speciosus) is common, but endemic to Japan, and its population structure was affected by habitat fragmentation because of urbanization. It suggested that the species might be one of the important models for the conservation of ecosystems and biodiversity affected by humans, including the effect of radioactive discharge caused by nuclear power plant accidents at Fukushima. We developed and characterized 25 novel polymorphic microsatellite markers from the next-generation sequencing data in an effort to provide an effective tool for genetic studies on this species. In 8 individuals from Aomori, Japan, the number of alleles and expected heterozygosities ranged from 5 to 13 and from 0.795 to 0.991, respectively, suggesting the availability of these markers for genetic studies in this species.

AB - The large Japanese wood mouse (Apodemus speciosus) is common, but endemic to Japan, and its population structure was affected by habitat fragmentation because of urbanization. It suggested that the species might be one of the important models for the conservation of ecosystems and biodiversity affected by humans, including the effect of radioactive discharge caused by nuclear power plant accidents at Fukushima. We developed and characterized 25 novel polymorphic microsatellite markers from the next-generation sequencing data in an effort to provide an effective tool for genetic studies on this species. In 8 individuals from Aomori, Japan, the number of alleles and expected heterozygosities ranged from 5 to 13 and from 0.795 to 0.991, respectively, suggesting the availability of these markers for genetic studies in this species.

KW - Apodemus speciosus

KW - Microsatellite

KW - NGS

KW - Wood mouse

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U2 - 10.1007/s12686-013-9953-1

DO - 10.1007/s12686-013-9953-1

M3 - 学術論文

AN - SCOPUS:84892880994

SN - 1877-7252

VL - 5

SP - 1001

EP - 1003

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 4

ER -

Isolation and characterization of 25 polymorphic microsatellites of the large Japanese wood mouse (Apodemus speciosus)

Abstract

Keywords

ASJC Scopus subject areas

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