Is the ClC-2 chloride channel involved in the Cl- secretory mechanism of gastric parietal cells?

Katsuhito Hori; Yuji Takahashi; Naoki Horikawa; Tetsushi Furukawa; Kazuhiro Tsukada; Noriaki Takeguchi; Hideki Sakai

doi:10.1016/j.febslet.2004.08.044

Is the ClC-2 chloride channel involved in the Cl^- secretory mechanism of gastric parietal cells?

Katsuhito Hori, Yuji Takahashi, Naoki Horikawa, Tetsushi Furukawa, Kazuhiro Tsukada, Noriaki Takeguchi, Hideki Sakai^*

^*Corresponding author for this work

Pharmaceutical Physiology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl^- channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H⁺,K⁺-ATPase. These results indicate that ClC-2 may not be a Cl^--transporting protein for gastric acid secretion in parietal cells.

Original language	English
Pages (from-to)	105-108
Number of pages	4
Journal	FEBS Letters
Volume	575
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.08.044
State	Published - 2004/09/24

Keywords

Chloride channel
ClC-2
Gastric acid

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2004.08.044

Cite this

@article{2a346718e8b24b38a5cc9fa82992259b,

title = "Is the ClC-2 chloride channel involved in the Cl- secretory mechanism of gastric parietal cells?",

abstract = "It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl- channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H+,K+-ATPase. These results indicate that ClC-2 may not be a Cl--transporting protein for gastric acid secretion in parietal cells.",

keywords = "Chloride channel, ClC-2, Gastric acid",

author = "Katsuhito Hori and Yuji Takahashi and Naoki Horikawa and Tetsushi Furukawa and Kazuhiro Tsukada and Noriaki Takeguchi and Hideki Sakai",

note = "Funding Information: We thank Drs. Brandee A. Hara and Judy G. Lee (University of Southern California) for careful reading of this manuscript. This work was supported in part by Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science (to H.S. and N.T.) and the Ministry of Education, Culture, Sports, Science and Technology of Japan (to H.S. and N.T.).",

year = "2004",

month = sep,

day = "24",

doi = "10.1016/j.febslet.2004.08.044",

language = "英語",

volume = "575",

pages = "105--108",

journal = "FEBS Letters",

issn = "0014-5793",