Involvement of auxin dynamics in hypergravity-induced promotion of lignin-related gene expression in Arabidopsis inflorescence stems

Daisuke Tamaoki; Ichirou Karahara; Takumi Nishiuchi; Tatsuya Wakasugi; Kyoji Yamada; Seiichiro Kamisaka

doi:10.1093/jxb/err224

Involvement of auxin dynamics in hypergravity-induced promotion of lignin-related gene expression in Arabidopsis inflorescence stems

Daisuke Tamaoki, Ichirou Karahara^*, Takumi Nishiuchi, Tatsuya Wakasugi, Kyoji Yamada, Seiichiro Kamisaka

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Recent studies have shown that hypergravity enhances lignification through up-regulation of the expression of lignin biosynthesis-related genes, although its hormonal signalling mechanism is unknown. The effects of hypergravity on auxin dynamics were examined using Arabidopsis plants that were transformed with the auxin reporter gene construct DR5::GUS. Hypergravity treatment at 300g significantly increased β-glucuronidase activity in inflorescence stems of DR5::GUS plants, indicating that endogenous auxin accumulation was enhanced by hypergravity treatment. The hypergravity-related increased expression levels of both DR5::GUS and lignin biosynthesis-related genes in inflorescence stems were suppressed after disbudding, indicating that the increased expression of lignin biosynthesis-related genes is dependent on an increase in auxin influx from the shoot apex.

Original language	English
Pages (from-to)	5463-5469
Number of pages	7
Journal	Journal of Experimental Botany
Volume	62
Issue number	15
DOIs	https://doi.org/10.1093/jxb/err224
State	Published - 2011/11

Keywords

Arabidopsis
DR5::GUS
auxin
disbudding
hypergravity
lignin

ASJC Scopus subject areas

Physiology
Plant Science

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10.1093/jxb/err224

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@article{f597639179ec4ae593188eaa8ba7abf2,

title = "Involvement of auxin dynamics in hypergravity-induced promotion of lignin-related gene expression in Arabidopsis inflorescence stems",

abstract = "Recent studies have shown that hypergravity enhances lignification through up-regulation of the expression of lignin biosynthesis-related genes, although its hormonal signalling mechanism is unknown. The effects of hypergravity on auxin dynamics were examined using Arabidopsis plants that were transformed with the auxin reporter gene construct DR5::GUS. Hypergravity treatment at 300g significantly increased β-glucuronidase activity in inflorescence stems of DR5::GUS plants, indicating that endogenous auxin accumulation was enhanced by hypergravity treatment. The hypergravity-related increased expression levels of both DR5::GUS and lignin biosynthesis-related genes in inflorescence stems were suppressed after disbudding, indicating that the increased expression of lignin biosynthesis-related genes is dependent on an increase in auxin influx from the shoot apex.",

keywords = "Arabidopsis, DR5::GUS, auxin, disbudding, hypergravity, lignin",

author = "Daisuke Tamaoki and Ichirou Karahara and Takumi Nishiuchi and Tatsuya Wakasugi and Kyoji Yamada and Seiichiro Kamisaka",

note = "Funding Information: We acknowledge Dr Thomas J. Guilfoyle of University of Missouri (Columbia, MO) for providing the DR5::GUS transgenic line. This work was supported fully by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (to DT), by a Grant-in-aid for Scientific Research nos 21570064 and 21657011 from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to IK), and {\textquoteleft}Ground-Based Research Announcement for Space Utilization{\textquoteright} funded by the Japan Space Forum (to SK and IK).",

year = "2011",

month = nov,

doi = "10.1093/jxb/err224",

language = "英語",

volume = "62",

pages = "5463--5469",

journal = "Journal of Experimental Botany",

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publisher = "Oxford University Press",

number = "15",

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TY - JOUR

T1 - Involvement of auxin dynamics in hypergravity-induced promotion of lignin-related gene expression in Arabidopsis inflorescence stems

AU - Tamaoki, Daisuke

AU - Karahara, Ichirou

AU - Nishiuchi, Takumi

AU - Wakasugi, Tatsuya

AU - Yamada, Kyoji

AU - Kamisaka, Seiichiro

N1 - Funding Information: We acknowledge Dr Thomas J. Guilfoyle of University of Missouri (Columbia, MO) for providing the DR5::GUS transgenic line. This work was supported fully by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (to DT), by a Grant-in-aid for Scientific Research nos 21570064 and 21657011 from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to IK), and ‘Ground-Based Research Announcement for Space Utilization’ funded by the Japan Space Forum (to SK and IK).

PY - 2011/11

Y1 - 2011/11

N2 - Recent studies have shown that hypergravity enhances lignification through up-regulation of the expression of lignin biosynthesis-related genes, although its hormonal signalling mechanism is unknown. The effects of hypergravity on auxin dynamics were examined using Arabidopsis plants that were transformed with the auxin reporter gene construct DR5::GUS. Hypergravity treatment at 300g significantly increased β-glucuronidase activity in inflorescence stems of DR5::GUS plants, indicating that endogenous auxin accumulation was enhanced by hypergravity treatment. The hypergravity-related increased expression levels of both DR5::GUS and lignin biosynthesis-related genes in inflorescence stems were suppressed after disbudding, indicating that the increased expression of lignin biosynthesis-related genes is dependent on an increase in auxin influx from the shoot apex.

AB - Recent studies have shown that hypergravity enhances lignification through up-regulation of the expression of lignin biosynthesis-related genes, although its hormonal signalling mechanism is unknown. The effects of hypergravity on auxin dynamics were examined using Arabidopsis plants that were transformed with the auxin reporter gene construct DR5::GUS. Hypergravity treatment at 300g significantly increased β-glucuronidase activity in inflorescence stems of DR5::GUS plants, indicating that endogenous auxin accumulation was enhanced by hypergravity treatment. The hypergravity-related increased expression levels of both DR5::GUS and lignin biosynthesis-related genes in inflorescence stems were suppressed after disbudding, indicating that the increased expression of lignin biosynthesis-related genes is dependent on an increase in auxin influx from the shoot apex.

KW - Arabidopsis

KW - DR5::GUS

KW - auxin

KW - disbudding

KW - hypergravity

KW - lignin

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U2 - 10.1093/jxb/err224

DO - 10.1093/jxb/err224

M3 - 学術論文

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AN - SCOPUS:82255192836

SN - 0022-0957

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SP - 5463

EP - 5469

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 15

ER -

Involvement of auxin dynamics in hypergravity-induced promotion of lignin-related gene expression in Arabidopsis inflorescence stems

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Keywords

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