Involvement of aquaporin-5 in differentiation of human gastric cancer cells

Tomoko Watanabe; Takuto Fujii; Takeshi Oya; Naoki Horikawa; Yoshiaki Tabuchi; Yuji Takahashi; Magotoshi Morii; Noriaki Takeguchi; Kazuhiro Tsukada; Hideki Sakai

doi:10.1007/s12576-008-0017-3

Involvement of aquaporin-5 in differentiation of human gastric cancer cells

Tomoko Watanabe, Takuto Fujii, Takeshi Oya, Naoki Horikawa, Yoshiaki Tabuchi, Yuji Takahashi, Magotoshi Morii, Noriaki Takeguchi, Kazuhiro Tsukada, Hideki Sakai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Litttle is known about the function of aquaporin (AQP) water channels in human gastric cancer. In the upper or middle part of human stomach, we found that expression level of AQP5 protein in intestinal type of adenocarcinoma was significantly higher than that in accompanying normal mucosa. AQP5 was localized in the apical membrane of the cancer cells. On the other hand, both AQP3 and AQP4 were not up-regulated in the adenocarcinoma. To elucidate the role of AQP5 in cancer cells, AQP5 was exogenously expressed in a cell line of poorly differentiated human gastric adenocarcinoma (MKN45). The AQP5 expression significantly increased the proportion of differentiated cells with a spindle shape, the activity of alkaline phosphatase, a marker for the intestinal epithelial cell type of cancer cells, and the expression level of laminin, an epithelial cell marker. Treatment of the MKN45 cells stably expressing AQP5 with HgCl₂, an inhibitor of aquaporins, significantly decreased the proportion of differentiated cells and the activity of alkaline phosphatase. Our results suggest that up-regulation of AQP5 may be involved in differentiation of human gastric cancer cells.

Original language	English
Pages (from-to)	113-122
Number of pages	10
Journal	Journal of Physiological Sciences
Volume	59
Issue number	2
DOIs	https://doi.org/10.1007/s12576-008-0017-3
State	Published - 2009/03

Keywords

Aquaporins
Cancer cells
Stomach

ASJC Scopus subject areas

General Medicine

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10.1007/s12576-008-0017-3

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@article{b18a3bfe8aec4f76943a59b7c03bf5af,

title = "Involvement of aquaporin-5 in differentiation of human gastric cancer cells",

abstract = "Litttle is known about the function of aquaporin (AQP) water channels in human gastric cancer. In the upper or middle part of human stomach, we found that expression level of AQP5 protein in intestinal type of adenocarcinoma was significantly higher than that in accompanying normal mucosa. AQP5 was localized in the apical membrane of the cancer cells. On the other hand, both AQP3 and AQP4 were not up-regulated in the adenocarcinoma. To elucidate the role of AQP5 in cancer cells, AQP5 was exogenously expressed in a cell line of poorly differentiated human gastric adenocarcinoma (MKN45). The AQP5 expression significantly increased the proportion of differentiated cells with a spindle shape, the activity of alkaline phosphatase, a marker for the intestinal epithelial cell type of cancer cells, and the expression level of laminin, an epithelial cell marker. Treatment of the MKN45 cells stably expressing AQP5 with HgCl2, an inhibitor of aquaporins, significantly decreased the proportion of differentiated cells and the activity of alkaline phosphatase. Our results suggest that up-regulation of AQP5 may be involved in differentiation of human gastric cancer cells.",

keywords = "Aquaporins, Cancer cells, Stomach",

author = "Tomoko Watanabe and Takuto Fujii and Takeshi Oya and Naoki Horikawa and Yoshiaki Tabuchi and Yuji Takahashi and Magotoshi Morii and Noriaki Takeguchi and Kazuhiro Tsukada and Hideki Sakai",

note = "Funding Information: Acknowledgments This work was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Mrs. Takako Matsushima for her excellent technical assistance for immunohistochemistry.",

year = "2009",

month = mar,

doi = "10.1007/s12576-008-0017-3",

language = "英語",

volume = "59",

pages = "113--122",

journal = "Journal of Physiological Sciences",

issn = "1880-6546",

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TY - JOUR

T1 - Involvement of aquaporin-5 in differentiation of human gastric cancer cells

AU - Watanabe, Tomoko

AU - Fujii, Takuto

AU - Oya, Takeshi

AU - Horikawa, Naoki

AU - Tabuchi, Yoshiaki

AU - Takahashi, Yuji

AU - Morii, Magotoshi

AU - Takeguchi, Noriaki

AU - Tsukada, Kazuhiro

AU - Sakai, Hideki

N1 - Funding Information: Acknowledgments This work was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Mrs. Takako Matsushima for her excellent technical assistance for immunohistochemistry.

PY - 2009/3

Y1 - 2009/3

N2 - Litttle is known about the function of aquaporin (AQP) water channels in human gastric cancer. In the upper or middle part of human stomach, we found that expression level of AQP5 protein in intestinal type of adenocarcinoma was significantly higher than that in accompanying normal mucosa. AQP5 was localized in the apical membrane of the cancer cells. On the other hand, both AQP3 and AQP4 were not up-regulated in the adenocarcinoma. To elucidate the role of AQP5 in cancer cells, AQP5 was exogenously expressed in a cell line of poorly differentiated human gastric adenocarcinoma (MKN45). The AQP5 expression significantly increased the proportion of differentiated cells with a spindle shape, the activity of alkaline phosphatase, a marker for the intestinal epithelial cell type of cancer cells, and the expression level of laminin, an epithelial cell marker. Treatment of the MKN45 cells stably expressing AQP5 with HgCl2, an inhibitor of aquaporins, significantly decreased the proportion of differentiated cells and the activity of alkaline phosphatase. Our results suggest that up-regulation of AQP5 may be involved in differentiation of human gastric cancer cells.

AB - Litttle is known about the function of aquaporin (AQP) water channels in human gastric cancer. In the upper or middle part of human stomach, we found that expression level of AQP5 protein in intestinal type of adenocarcinoma was significantly higher than that in accompanying normal mucosa. AQP5 was localized in the apical membrane of the cancer cells. On the other hand, both AQP3 and AQP4 were not up-regulated in the adenocarcinoma. To elucidate the role of AQP5 in cancer cells, AQP5 was exogenously expressed in a cell line of poorly differentiated human gastric adenocarcinoma (MKN45). The AQP5 expression significantly increased the proportion of differentiated cells with a spindle shape, the activity of alkaline phosphatase, a marker for the intestinal epithelial cell type of cancer cells, and the expression level of laminin, an epithelial cell marker. Treatment of the MKN45 cells stably expressing AQP5 with HgCl2, an inhibitor of aquaporins, significantly decreased the proportion of differentiated cells and the activity of alkaline phosphatase. Our results suggest that up-regulation of AQP5 may be involved in differentiation of human gastric cancer cells.

KW - Aquaporins

KW - Cancer cells

KW - Stomach

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DO - 10.1007/s12576-008-0017-3

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ER -

Involvement of aquaporin-5 in differentiation of human gastric cancer cells

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