TY - JOUR
T1 - Intratumoral heterogeneity of HER2 protein and amplification of HER2 gene in salivary duct carcinoma
AU - Kondo, Yusuke
AU - Kikuchi, Tomoki
AU - Esteban, Joaquim Carreras
AU - Kumaki, Nobue
AU - Ogura, Go
AU - Inomoto, Chie
AU - Hirabayashi, Kenichi
AU - Kajiwara, Hiroshi
AU - Sakai, Akihiro
AU - Sugimoto, Ryousuke
AU - Otsuru, Mitsunobu
AU - Okami, Kenji
AU - Tsukinoki, Keiichi
AU - Nakamura, Naoya
N1 - Publisher Copyright:
© 2014 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Salivary duct carcinoma (SDC) is an aggressive adenocarcinoma of the salivary glands, and accounts for 1-3% of all malignant salivary gland tumors, resembling morphologically invasive ductal carcinoma (IDC) of the breast. In contrast to IDC of the breast and gastric carcinoma (GC), the study of human epidermal growth factor receptor 2 (HER2) in SDC has not progressed. Therefore, we investigated the relationship between HER2 protein expression and amplification of the HER2 gene, and compared them in terms of intratumoral heterogeneity (ITH) in 13 cases of SDC using immunohistochemistry and dual color in situ hybridization. We found seven cases with protein overexpression (53.8%) and five cases with gene amplification (38.5%) in accordance with ASCO/CAP guidelines. ITH of HER2 protein expression was seen in seven cases (53.8%). Interestingly, the ratio of the HER2 gene showed homogenous distribution with or without the presence of ITH of HER2 protein expression. SDC tends to have more ITH of HER2 protein similarly to GC, in contrast to IDC of the breast. ITH of HER2 protein in SDC has no heterogeneity of the HER2 gene amplification. The mechanism of HER2 protein expression in SDC might proceed through a more complex pathway relative to that of IDC of the breast.
AB - Salivary duct carcinoma (SDC) is an aggressive adenocarcinoma of the salivary glands, and accounts for 1-3% of all malignant salivary gland tumors, resembling morphologically invasive ductal carcinoma (IDC) of the breast. In contrast to IDC of the breast and gastric carcinoma (GC), the study of human epidermal growth factor receptor 2 (HER2) in SDC has not progressed. Therefore, we investigated the relationship between HER2 protein expression and amplification of the HER2 gene, and compared them in terms of intratumoral heterogeneity (ITH) in 13 cases of SDC using immunohistochemistry and dual color in situ hybridization. We found seven cases with protein overexpression (53.8%) and five cases with gene amplification (38.5%) in accordance with ASCO/CAP guidelines. ITH of HER2 protein expression was seen in seven cases (53.8%). Interestingly, the ratio of the HER2 gene showed homogenous distribution with or without the presence of ITH of HER2 protein expression. SDC tends to have more ITH of HER2 protein similarly to GC, in contrast to IDC of the breast. ITH of HER2 protein in SDC has no heterogeneity of the HER2 gene amplification. The mechanism of HER2 protein expression in SDC might proceed through a more complex pathway relative to that of IDC of the breast.
KW - Heterogeneity
KW - Human epidermal growth factor receptor 2 (HER2)
KW - Salivary duct carcinoma (SDC)
UR - http://www.scopus.com/inward/record.url?scp=84927789044&partnerID=8YFLogxK
U2 - 10.1111/pin.12195
DO - 10.1111/pin.12195
M3 - 学術論文
C2 - 25209856
AN - SCOPUS:84927789044
SN - 1320-5463
VL - 64
SP - 453
EP - 459
JO - Pathology International
JF - Pathology International
IS - 9
ER -
