Inkjetting Plus Peroxidase-Mediated Hydrogelation Produces Cell-Laden, Cell-Sized Particles with Suitable Characters for Individual Applications

Shinji Sakai; Yusuke Yamamoto; Gantumur Enkhtuul; Kohei Ueda; Kenichi Arai; Masahito Taya; Makoto Nakamura

doi:10.1002/mabi.201600416

Inkjetting Plus Peroxidase-Mediated Hydrogelation Produces Cell-Laden, Cell-Sized Particles with Suitable Characters for Individual Applications

Shinji Sakai^*, Yusuke Yamamoto, Gantumur Enkhtuul, Kohei Ueda, Kenichi Arai, Masahito Taya, Makoto Nakamura

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The authors report a method to prepare cell-laden, cell-sized microparticles from various materials suitable for individual applications. The method includes a piezoelectric inkjetting technology and a horseradish peroxidase (HRP)-catalyzed crosslinking reaction. The piezoelectric inkjetting technology enables production of cell-laden, cell-sized (20–60 μm) droplets from a polymer aqueous solution. The HRP-catalyzed crosslinking of the polymer in the ejected solution enables production of spherical microparticles from various materials. Superior cytocompatibility of the microencapsulation method is confirmed from the viability and growth profiles of normal murine mammary gland epithelial cells. (Figure presented.).

Original language	English
Article number	1600416
Journal	Macromolecular Bioscience
Volume	17
Issue number	5
DOIs	https://doi.org/10.1002/mabi.201600416
State	Published - 2017/05/01

Keywords

cell-laden microparticles
horseradish peroxidase
hydrogels
inkjetting
microencapsulation

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1002/mabi.201600416

Cite this

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title = "Inkjetting Plus Peroxidase-Mediated Hydrogelation Produces Cell-Laden, Cell-Sized Particles with Suitable Characters for Individual Applications",

abstract = "The authors report a method to prepare cell-laden, cell-sized microparticles from various materials suitable for individual applications. The method includes a piezoelectric inkjetting technology and a horseradish peroxidase (HRP)-catalyzed crosslinking reaction. The piezoelectric inkjetting technology enables production of cell-laden, cell-sized (20–60 μm) droplets from a polymer aqueous solution. The HRP-catalyzed crosslinking of the polymer in the ejected solution enables production of spherical microparticles from various materials. Superior cytocompatibility of the microencapsulation method is confirmed from the viability and growth profiles of normal murine mammary gland epithelial cells. (Figure presented.).",

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author = "Shinji Sakai and Yusuke Yamamoto and Gantumur Enkhtuul and Kohei Ueda and Kenichi Arai and Masahito Taya and Makoto Nakamura",

note = "Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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doi = "10.1002/mabi.201600416",

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AU - Sakai, Shinji

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AU - Enkhtuul, Gantumur

AU - Ueda, Kohei

AU - Arai, Kenichi

AU - Taya, Masahito

AU - Nakamura, Makoto

PY - 2017/5/1

Y1 - 2017/5/1

N2 - The authors report a method to prepare cell-laden, cell-sized microparticles from various materials suitable for individual applications. The method includes a piezoelectric inkjetting technology and a horseradish peroxidase (HRP)-catalyzed crosslinking reaction. The piezoelectric inkjetting technology enables production of cell-laden, cell-sized (20–60 μm) droplets from a polymer aqueous solution. The HRP-catalyzed crosslinking of the polymer in the ejected solution enables production of spherical microparticles from various materials. Superior cytocompatibility of the microencapsulation method is confirmed from the viability and growth profiles of normal murine mammary gland epithelial cells. (Figure presented.).

AB - The authors report a method to prepare cell-laden, cell-sized microparticles from various materials suitable for individual applications. The method includes a piezoelectric inkjetting technology and a horseradish peroxidase (HRP)-catalyzed crosslinking reaction. The piezoelectric inkjetting technology enables production of cell-laden, cell-sized (20–60 μm) droplets from a polymer aqueous solution. The HRP-catalyzed crosslinking of the polymer in the ejected solution enables production of spherical microparticles from various materials. Superior cytocompatibility of the microencapsulation method is confirmed from the viability and growth profiles of normal murine mammary gland epithelial cells. (Figure presented.).

KW - cell-laden microparticles

KW - horseradish peroxidase

KW - hydrogels

KW - inkjetting

KW - microencapsulation

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Inkjetting Plus Peroxidase-Mediated Hydrogelation Produces Cell-Laden, Cell-Sized Particles with Suitable Characters for Individual Applications

Abstract

Keywords

ASJC Scopus subject areas

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