TY - JOUR
T1 - Inhibitory effect of TNF-α produced by macrophages stimulated with Grifola frondosa extract (ME) on the growth of influenza A/Aichi/2/68 Virus in MDCK cells
AU - Obi, Nobuko
AU - Hayashi, Katsumi
AU - Miyahara, Tatsurou
AU - Shimada, Yutaka
AU - Terasawa, Katsutoshi
AU - Watanabe, Masataka
AU - Takeyama, Masahide
AU - Obi, Ryosuke
AU - Ochiai, Hiroshi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for the 21st Century COE Program from the Ministry Education, Culture, Sports, Science and Technology, Japan.
PY - 2008
Y1 - 2008
N2 - We investigated the inhibitory effect of the conditioned medium (CM) from P338D1 (D1) cells, a murine macrophage cell line, stimulated for 10 hours with a fixed dose (100 μg/ml) of the extracts from the fruit bodies of Grifola frondosa (ME) or its ultra filtration-based fractions (MFs), on the growth of influenza A/Aichi/2/68 virus in Madin-Darby canine kidney cells. Direct addition of ME and 3 kinds of MFs (MF1, MF2 and MF3) to the infected cells had no obvious inhibitory effect. However, virus yields were reduced in the presence of CMs. Notably, the inhibitory effect of the CM prepared by using MF2 (molecular weight of 30 Kd to 100 Kd) was the strongest (28% reduction compared to the control). RT-PCR and ELISA assays showed that the CMs could induce the expression of TNF-α mRNA in D1 cells leading to production of TNF-α, known as an antiviral cytokine. These findings suggest that ME and MFs (especially MF-2) might induce the production of certain factors, including TNF-α, which are responsible for the inhibition of viral growth in vitro.
AB - We investigated the inhibitory effect of the conditioned medium (CM) from P338D1 (D1) cells, a murine macrophage cell line, stimulated for 10 hours with a fixed dose (100 μg/ml) of the extracts from the fruit bodies of Grifola frondosa (ME) or its ultra filtration-based fractions (MFs), on the growth of influenza A/Aichi/2/68 virus in Madin-Darby canine kidney cells. Direct addition of ME and 3 kinds of MFs (MF1, MF2 and MF3) to the infected cells had no obvious inhibitory effect. However, virus yields were reduced in the presence of CMs. Notably, the inhibitory effect of the CM prepared by using MF2 (molecular weight of 30 Kd to 100 Kd) was the strongest (28% reduction compared to the control). RT-PCR and ELISA assays showed that the CMs could induce the expression of TNF-α mRNA in D1 cells leading to production of TNF-α, known as an antiviral cytokine. These findings suggest that ME and MFs (especially MF-2) might induce the production of certain factors, including TNF-α, which are responsible for the inhibition of viral growth in vitro.
KW - Conditioned medium
KW - Extract of grifola frondosa
KW - Influenza virus
KW - Macrophage
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=57349108596&partnerID=8YFLogxK
U2 - 10.1142/S0192415X08006508
DO - 10.1142/S0192415X08006508
M3 - 学術論文
C2 - 19051344
AN - SCOPUS:57349108596
SN - 0192-415X
VL - 36
SP - 1171
EP - 1183
JO - American Journal of Chinese Medicine
JF - American Journal of Chinese Medicine
IS - 6
ER -