Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast

Chor Wai Lo; Daisuke Kaida; Shinichi Nishimura; Akihisa Matsuyama; Yoko Yashiroda; Hiroshi Taoka; Ken Ishigami; Hidenori Watanabe; Hidenori Nakajima; Tokio Tani; Sueharu Horinouchi; Minoru Yoshida

doi:10.1016/j.bbrc.2007.10.029

Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast

Chor Wai Lo, Daisuke Kaida, Shinichi Nishimura, Akihisa Matsuyama, Yoko Yashiroda, Hiroshi Taoka, Ken Ishigami, Hidenori Watanabe, Hidenori Nakajima, Tokio Tani, Sueharu Horinouchi, Minoru Yoshida^*

^*Corresponding author for this work

Gene Expression and Regulation

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Nuclear retention of pre-mRNAs is tightly regulated by several security mechanisms that prevent pre-mRNA export into the cytoplasm. Recently, spliceostatin A, a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. Here we report that spliceostatin A also inhibits splicing and nuclear retention of pre-mRNA in a fission yeast strain that lacks the multidrug resistance protein Pmd1. As observed in mammalian cells, spliceostatin A is bound to components of the SF3b complex in the spliceosome. Furthermore, overexpression of nup211, a homolog of Saccharomyces cerevisiae MLP1, suppresses translation of pre-mRNAs accumulated by spliceostatin A. These results suggest that the SF3b complex has a conserved role in pre-mRNA retention, which is independent of the Mlp1 function.

Original language	English
Pages (from-to)	573-577
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	364
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.10.029
State	Published - 2007/12/21

Keywords

Fission yeast
Pre-mRNA
Pre-mRNA retention
SF3b
Spliceostatin A
Splicing

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.10.029

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Lo, C. W., Kaida, D., Nishimura, S., Matsuyama, A., Yashiroda, Y., Taoka, H., Ishigami, K., Watanabe, H., Nakajima, H., Tani, T., Horinouchi, S., & Yoshida, M. (2007). Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast. Biochemical and Biophysical Research Communications, 364(3), 573-577. https://doi.org/10.1016/j.bbrc.2007.10.029

@article{214ab6cd2d054dc78df8b181b8ac41fe,

title = "Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast",

abstract = "Nuclear retention of pre-mRNAs is tightly regulated by several security mechanisms that prevent pre-mRNA export into the cytoplasm. Recently, spliceostatin A, a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. Here we report that spliceostatin A also inhibits splicing and nuclear retention of pre-mRNA in a fission yeast strain that lacks the multidrug resistance protein Pmd1. As observed in mammalian cells, spliceostatin A is bound to components of the SF3b complex in the spliceosome. Furthermore, overexpression of nup211, a homolog of Saccharomyces cerevisiae MLP1, suppresses translation of pre-mRNAs accumulated by spliceostatin A. These results suggest that the SF3b complex has a conserved role in pre-mRNA retention, which is independent of the Mlp1 function.",

keywords = "Fission yeast, Pre-mRNA, Pre-mRNA retention, SF3b, Spliceostatin A, Splicing",

author = "Lo, {Chor Wai} and Daisuke Kaida and Shinichi Nishimura and Akihisa Matsuyama and Yoko Yashiroda and Hiroshi Taoka and Ken Ishigami and Hidenori Watanabe and Hidenori Nakajima and Tokio Tani and Sueharu Horinouchi and Minoru Yoshida",

note = "Funding Information: We are grateful to the RIKEN Brain Science Institute{\textquoteright}s Research Resources Center for DNA sequencing analysis. This work was supported in part by the CREST Research Project, the Japan Science and Technology Agency, The Strategic Research Programs for R&D, RIKEN, and a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan. ",

year = "2007",

month = dec,

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doi = "10.1016/j.bbrc.2007.10.029",

language = "英語",

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pages = "573--577",

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issn = "0006-291X",

publisher = "Elsevier B.V.",

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Lo, CW, Kaida, D, Nishimura, S, Matsuyama, A, Yashiroda, Y, Taoka, H, Ishigami, K, Watanabe, H, Nakajima, H, Tani, T, Horinouchi, S & Yoshida, M 2007, 'Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast', Biochemical and Biophysical Research Communications, vol. 364, no. 3, pp. 573-577. https://doi.org/10.1016/j.bbrc.2007.10.029

TY - JOUR

T1 - Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast

AU - Lo, Chor Wai

AU - Kaida, Daisuke

AU - Nishimura, Shinichi

AU - Matsuyama, Akihisa

AU - Yashiroda, Yoko

AU - Taoka, Hiroshi

AU - Ishigami, Ken

AU - Watanabe, Hidenori

AU - Nakajima, Hidenori

AU - Tani, Tokio

AU - Horinouchi, Sueharu

AU - Yoshida, Minoru

N1 - Funding Information: We are grateful to the RIKEN Brain Science Institute’s Research Resources Center for DNA sequencing analysis. This work was supported in part by the CREST Research Project, the Japan Science and Technology Agency, The Strategic Research Programs for R&D, RIKEN, and a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2007/12/21

Y1 - 2007/12/21

N2 - Nuclear retention of pre-mRNAs is tightly regulated by several security mechanisms that prevent pre-mRNA export into the cytoplasm. Recently, spliceostatin A, a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. Here we report that spliceostatin A also inhibits splicing and nuclear retention of pre-mRNA in a fission yeast strain that lacks the multidrug resistance protein Pmd1. As observed in mammalian cells, spliceostatin A is bound to components of the SF3b complex in the spliceosome. Furthermore, overexpression of nup211, a homolog of Saccharomyces cerevisiae MLP1, suppresses translation of pre-mRNAs accumulated by spliceostatin A. These results suggest that the SF3b complex has a conserved role in pre-mRNA retention, which is independent of the Mlp1 function.

AB - Nuclear retention of pre-mRNAs is tightly regulated by several security mechanisms that prevent pre-mRNA export into the cytoplasm. Recently, spliceostatin A, a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. Here we report that spliceostatin A also inhibits splicing and nuclear retention of pre-mRNA in a fission yeast strain that lacks the multidrug resistance protein Pmd1. As observed in mammalian cells, spliceostatin A is bound to components of the SF3b complex in the spliceosome. Furthermore, overexpression of nup211, a homolog of Saccharomyces cerevisiae MLP1, suppresses translation of pre-mRNAs accumulated by spliceostatin A. These results suggest that the SF3b complex has a conserved role in pre-mRNA retention, which is independent of the Mlp1 function.

KW - Fission yeast

KW - Pre-mRNA

KW - Pre-mRNA retention

KW - SF3b

KW - Spliceostatin A

KW - Splicing

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U2 - 10.1016/j.bbrc.2007.10.029

DO - 10.1016/j.bbrc.2007.10.029

M3 - 学術論文

C2 - 17961508

AN - SCOPUS:35648965653

SN - 0006-291X

VL - 364

SP - 573

EP - 577

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast

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Keywords

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