TY - JOUR
T1 - Inhibition of PAH transport by parathyroid hormone in OK cells
T2 - Involvement of protein kinase C pathway
AU - Nagai, Junya
AU - Yano, Ikuko
AU - Hashimoto, Yukiya
AU - Takano, Mikihisa
AU - Inui, Ken Ichi
PY - 1997
Y1 - 1997
N2 - We have previously shown that the p-aminohippurate (PAH) transport system in OK kidney epithelial cell line is under the regulatory control of protein kinase C. Parathyroid hormone (PTH) could activate protein kinase C, as well as protein kinase A, in OK cells. In the present study, the effect of PTH on PAH transport was studied in OK cells. PTH inhibited the transcellular transport of PAH from the basal to the apical side, as well as the accumulation of PAH in OK cells. Basolateral PAH uptake was inhibited by PTH in a dose- and time-dependent manner. Protein kinase A activators did not affect the transcellular transport or the accumulation of PAH. The PTH- induced inhibition of the accumulation of PAH was blocked by a protein kinase C inhibitor staurosporine. These results suggest that PTH inhibits the PAH transport in OK cells and that the messenger system mediated by protein kinase C, not protein kinase A, plays an important role in the regulation of PAH transport by PTH.
AB - We have previously shown that the p-aminohippurate (PAH) transport system in OK kidney epithelial cell line is under the regulatory control of protein kinase C. Parathyroid hormone (PTH) could activate protein kinase C, as well as protein kinase A, in OK cells. In the present study, the effect of PTH on PAH transport was studied in OK cells. PTH inhibited the transcellular transport of PAH from the basal to the apical side, as well as the accumulation of PAH in OK cells. Basolateral PAH uptake was inhibited by PTH in a dose- and time-dependent manner. Protein kinase A activators did not affect the transcellular transport or the accumulation of PAH. The PTH- induced inhibition of the accumulation of PAH was blocked by a protein kinase C inhibitor staurosporine. These results suggest that PTH inhibits the PAH transport in OK cells and that the messenger system mediated by protein kinase C, not protein kinase A, plays an important role in the regulation of PAH transport by PTH.
KW - Hormonal control
KW - Opossum kidney cell
KW - Organic anion transport
KW - Renal secretion
UR - http://www.scopus.com/inward/record.url?scp=0030669602&partnerID=8YFLogxK
U2 - 10.1152/ajprenal.1997.273.5.f674
DO - 10.1152/ajprenal.1997.273.5.f674
M3 - 学術論文
C2 - 9374830
AN - SCOPUS:0030669602
SN - 1931-857X
VL - 273
SP - F674-F679
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 5 42-5
ER -