Inhibition of PAH transport by parathyroid hormone in OK cells: Involvement of protein kinase C pathway

Junya Nagai, Ikuko Yano, Yukiya Hashimoto, Mikihisa Takano, Ken Ichi Inui*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

We have previously shown that the p-aminohippurate (PAH) transport system in OK kidney epithelial cell line is under the regulatory control of protein kinase C. Parathyroid hormone (PTH) could activate protein kinase C, as well as protein kinase A, in OK cells. In the present study, the effect of PTH on PAH transport was studied in OK cells. PTH inhibited the transcellular transport of PAH from the basal to the apical side, as well as the accumulation of PAH in OK cells. Basolateral PAH uptake was inhibited by PTH in a dose- and time-dependent manner. Protein kinase A activators did not affect the transcellular transport or the accumulation of PAH. The PTH- induced inhibition of the accumulation of PAH was blocked by a protein kinase C inhibitor staurosporine. These results suggest that PTH inhibits the PAH transport in OK cells and that the messenger system mediated by protein kinase C, not protein kinase A, plays an important role in the regulation of PAH transport by PTH.

Original languageEnglish
Pages (from-to)F674-F679
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number5 42-5
DOIs
StatePublished - 1997

Keywords

  • Hormonal control
  • Opossum kidney cell
  • Organic anion transport
  • Renal secretion

ASJC Scopus subject areas

  • Physiology
  • Urology

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