Influence of an adenosine deaminase inhibitor, erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride, on 5-HT2CR mRNA editing in primary cultured cortical cells

Pham Nguyet Thi Hang; Michihisa Tohda; Yasuhiro Tezuka; Kinzo Matsumoto

doi:10.1248/bpb.33.527

Influence of an adenosine deaminase inhibitor, erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride, on 5-HT2CR mRNA editing in primary cultured cortical cells

Pham Nguyet Thi Hang, Michihisa Tohda^*, Yasuhiro Tezuka, Kinzo Matsumoto

^*Corresponding author for this work

Section of Neuromedical Science, Division of Bioscience, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Treatment of primary cultured cortical cells with erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride (EHNA), an inhibitor of adenosine deaminase (ADAR), for 6 d significantly and concentration-dependently reduced the editing efficacy at sites C and D but not at site A or B of 5-HT2CR mRNA. The treatment failed to affect the editing of ADAR-2 pre-mRNA and a subunit of the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor (GluR2) mRNA. These findings suggest that EHNA is useful for clarifying the functional roles of 5-HT2CR mRNA editing at sites C and D.

Original language	English
Pages (from-to)	527-529
Number of pages	3
Journal	Biological and Pharmaceutical Bulletin
Volume	33
Issue number	3
DOIs	https://doi.org/10.1248/bpb.33.527
State	Published - 2010/03

Keywords

5-HT2C receptor mRNA editing
Adenosine deaminase
Psychiatric disorder

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

Access to Document

10.1248/bpb.33.527

Cite this

@article{6268390a3d4c43d8a1b29ad483ae29af,

title = "Influence of an adenosine deaminase inhibitor, erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride, on 5-HT2CR mRNA editing in primary cultured cortical cells",

abstract = "Treatment of primary cultured cortical cells with erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride (EHNA), an inhibitor of adenosine deaminase (ADAR), for 6 d significantly and concentration-dependently reduced the editing efficacy at sites C and D but not at site A or B of 5-HT2CR mRNA. The treatment failed to affect the editing of ADAR-2 pre-mRNA and a subunit of the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor (GluR2) mRNA. These findings suggest that EHNA is useful for clarifying the functional roles of 5-HT2CR mRNA editing at sites C and D.",

keywords = "5-HT2C receptor mRNA editing, Adenosine deaminase, Psychiatric disorder",

author = "Hang, {Pham Nguyet Thi} and Michihisa Tohda and Yasuhiro Tezuka and Kinzo Matsumoto",

year = "2010",

month = mar,

doi = "10.1248/bpb.33.527",

language = "英語",

volume = "33",

pages = "527--529",

journal = "Biological and Pharmaceutical Bulletin",

issn = "0918-6158",

publisher = "Pharmaceutical Society of Japan",

number = "3",

}

Influence of an adenosine deaminase inhibitor, erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride, on 5-HT2CR mRNA editing in primary cultured cortical cells. / Hang, Pham Nguyet Thi; Tohda, Michihisa; Tezuka, Yasuhiro et al.
In: Biological and Pharmaceutical Bulletin, Vol. 33, No. 3, 03.2010, p. 527-529.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Influence of an adenosine deaminase inhibitor, erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride, on 5-HT2CR mRNA editing in primary cultured cortical cells

AU - Hang, Pham Nguyet Thi

AU - Tohda, Michihisa

AU - Tezuka, Yasuhiro

AU - Matsumoto, Kinzo

PY - 2010/3

Y1 - 2010/3

N2 - Treatment of primary cultured cortical cells with erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride (EHNA), an inhibitor of adenosine deaminase (ADAR), for 6 d significantly and concentration-dependently reduced the editing efficacy at sites C and D but not at site A or B of 5-HT2CR mRNA. The treatment failed to affect the editing of ADAR-2 pre-mRNA and a subunit of the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor (GluR2) mRNA. These findings suggest that EHNA is useful for clarifying the functional roles of 5-HT2CR mRNA editing at sites C and D.

AB - Treatment of primary cultured cortical cells with erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride (EHNA), an inhibitor of adenosine deaminase (ADAR), for 6 d significantly and concentration-dependently reduced the editing efficacy at sites C and D but not at site A or B of 5-HT2CR mRNA. The treatment failed to affect the editing of ADAR-2 pre-mRNA and a subunit of the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor (GluR2) mRNA. These findings suggest that EHNA is useful for clarifying the functional roles of 5-HT2CR mRNA editing at sites C and D.

KW - 5-HT2C receptor mRNA editing

KW - Adenosine deaminase

KW - Psychiatric disorder

UR - http://www.scopus.com/inward/record.url?scp=77649228878&partnerID=8YFLogxK

U2 - 10.1248/bpb.33.527

DO - 10.1248/bpb.33.527

M3 - 学術論文

C2 - 20190421

AN - SCOPUS:77649228878

SN - 0918-6158

VL - 33

SP - 527

EP - 529

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

IS - 3

ER -

Influence of an adenosine deaminase inhibitor, erythro-9-(2-hydroxy-3- nonyl) adenine hydrochloride, on 5-HT2CR mRNA editing in primary cultured cortical cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this