In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA

Shoki Yashiki; Katsuhiro Ogawa; Hidefumi Shiroshita; Masafumi Inomata; Takashi Katagiri; Yusuke Oshima

doi:10.1117/12.3045327

In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA

Shoki Yashiki^*, Katsuhiro Ogawa, Hidefumi Shiroshita, Masafumi Inomata, Takashi Katagiri, Yusuke Oshima

^*Corresponding author for this work

Intellectual Information Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Photodynamic Therapy (PDT) is one of the minimally invasive therapies for early stage of cancers. 5-Aminolevulinic acid (5-ALA) is a photosensitizer that is clinically used in PDT. In our country, 5-ALA has been approved and used as a photosensitizer for photodynamic diagnosis (PDD), but not for PDT. The conventional procedure of 5-ALA-PDT using visible light sources has problems with the spatial selectivity, specificity and penetration depth onto cancer cells in the tissue. To overcome this, we evaluated the phototoxicity in tumor cells under 5-ALA treatment by two-photon excitation for the indication expansion of PDD/PDT in in vitro condition. In this study, a gastric cancer cell line MKN74 treated with 5-ALA was microscopically irradiated with a near-infrared ultrafast pulse laser and assessed the cell viability. In the result, the florescence signal and cell death successfully occurred by two-photon excitation, but the photobleaching is significantly high in the areas where the cells survived. To improve the therapeutic efficiency, further optimization of laser parameters such as average power, pulse width, repetition rate, and the irradiation strategies in vitro and in vivo.

Original language	English
Title of host publication	Optical Methods for Tumor Treatment and Detection
Subtitle of host publication	Mechanisms and Techniques in Photodynamic Therapy XXXIII
Editors	David H. Kessel, Tayyaba Hasan, Tayyaba Hasan, Tayyaba Hasan, Edward V. Maytin
Publisher	SPIE
ISBN (Electronic)	9781510683464
DOIs	https://doi.org/10.1117/12.3045327
State	Published - 2025
Event	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII 2025 - San Francisco, United States Duration: 2025/01/25 → 2025/01/27

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	13299
ISSN (Print)	1605-7422

Conference

Conference	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII 2025
Country/Territory	United States
City	San Francisco
Period	2025/01/25 → 2025/01/27

Keywords

5-ALA
Multiphoton excitation
NIR-ultrafast laser pulses
PDD
PDT

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.3045327

Cite this

Yashiki, S., Ogawa, K., Shiroshita, H., Inomata, M., Katagiri, T., & Oshima, Y. (2025). In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA. In D. H. Kessel, T. Hasan, T. Hasan, T. Hasan, & E. V. Maytin (Eds.), Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII Article 1329903 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13299). SPIE. https://doi.org/10.1117/12.3045327

Yashiki, Shoki ; Ogawa, Katsuhiro ; Shiroshita, Hidefumi et al. / In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA. Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII. editor / David H. Kessel ; Tayyaba Hasan ; Tayyaba Hasan ; Tayyaba Hasan ; Edward V. Maytin. SPIE, 2025. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{d11f753bcd854b639288ee90013d3cc6,

title = "In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA",

abstract = "Photodynamic Therapy (PDT) is one of the minimally invasive therapies for early stage of cancers. 5-Aminolevulinic acid (5-ALA) is a photosensitizer that is clinically used in PDT. In our country, 5-ALA has been approved and used as a photosensitizer for photodynamic diagnosis (PDD), but not for PDT. The conventional procedure of 5-ALA-PDT using visible light sources has problems with the spatial selectivity, specificity and penetration depth onto cancer cells in the tissue. To overcome this, we evaluated the phototoxicity in tumor cells under 5-ALA treatment by two-photon excitation for the indication expansion of PDD/PDT in in vitro condition. In this study, a gastric cancer cell line MKN74 treated with 5-ALA was microscopically irradiated with a near-infrared ultrafast pulse laser and assessed the cell viability. In the result, the florescence signal and cell death successfully occurred by two-photon excitation, but the photobleaching is significantly high in the areas where the cells survived. To improve the therapeutic efficiency, further optimization of laser parameters such as average power, pulse width, repetition rate, and the irradiation strategies in vitro and in vivo.",

keywords = "5-ALA, Multiphoton excitation, NIR-ultrafast laser pulses, PDD, PDT",

author = "Shoki Yashiki and Katsuhiro Ogawa and Hidefumi Shiroshita and Masafumi Inomata and Takashi Katagiri and Yusuke Oshima",

note = "Publisher Copyright: {\textcopyright} 2025 SPIE.; Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII 2025 ; Conference date: 25-01-2025 Through 27-01-2025",

year = "2025",

doi = "10.1117/12.3045327",

language = "英語",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Kessel, {David H.} and Tayyaba Hasan and Tayyaba Hasan and Tayyaba Hasan and Maytin, {Edward V.}",

booktitle = "Optical Methods for Tumor Treatment and Detection",

}

Yashiki, S, Ogawa, K, Shiroshita, H, Inomata, M, Katagiri, T & Oshima, Y 2025, In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA. in DH Kessel, T Hasan, T Hasan, T Hasan & EV Maytin (eds), Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII., 1329903, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 13299, SPIE, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII 2025, San Francisco, United States, 2025/01/25. https://doi.org/10.1117/12.3045327

In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA. / Yashiki, Shoki; Ogawa, Katsuhiro; Shiroshita, Hidefumi et al.
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII. ed. / David H. Kessel; Tayyaba Hasan; Tayyaba Hasan; Tayyaba Hasan; Edward V. Maytin. SPIE, 2025. 1329903 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13299).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA

AU - Yashiki, Shoki

AU - Ogawa, Katsuhiro

AU - Shiroshita, Hidefumi

AU - Inomata, Masafumi

AU - Katagiri, Takashi

AU - Oshima, Yusuke

PY - 2025

Y1 - 2025

N2 - Photodynamic Therapy (PDT) is one of the minimally invasive therapies for early stage of cancers. 5-Aminolevulinic acid (5-ALA) is a photosensitizer that is clinically used in PDT. In our country, 5-ALA has been approved and used as a photosensitizer for photodynamic diagnosis (PDD), but not for PDT. The conventional procedure of 5-ALA-PDT using visible light sources has problems with the spatial selectivity, specificity and penetration depth onto cancer cells in the tissue. To overcome this, we evaluated the phototoxicity in tumor cells under 5-ALA treatment by two-photon excitation for the indication expansion of PDD/PDT in in vitro condition. In this study, a gastric cancer cell line MKN74 treated with 5-ALA was microscopically irradiated with a near-infrared ultrafast pulse laser and assessed the cell viability. In the result, the florescence signal and cell death successfully occurred by two-photon excitation, but the photobleaching is significantly high in the areas where the cells survived. To improve the therapeutic efficiency, further optimization of laser parameters such as average power, pulse width, repetition rate, and the irradiation strategies in vitro and in vivo.

AB - Photodynamic Therapy (PDT) is one of the minimally invasive therapies for early stage of cancers. 5-Aminolevulinic acid (5-ALA) is a photosensitizer that is clinically used in PDT. In our country, 5-ALA has been approved and used as a photosensitizer for photodynamic diagnosis (PDD), but not for PDT. The conventional procedure of 5-ALA-PDT using visible light sources has problems with the spatial selectivity, specificity and penetration depth onto cancer cells in the tissue. To overcome this, we evaluated the phototoxicity in tumor cells under 5-ALA treatment by two-photon excitation for the indication expansion of PDD/PDT in in vitro condition. In this study, a gastric cancer cell line MKN74 treated with 5-ALA was microscopically irradiated with a near-infrared ultrafast pulse laser and assessed the cell viability. In the result, the florescence signal and cell death successfully occurred by two-photon excitation, but the photobleaching is significantly high in the areas where the cells survived. To improve the therapeutic efficiency, further optimization of laser parameters such as average power, pulse width, repetition rate, and the irradiation strategies in vitro and in vivo.

KW - 5-ALA

KW - Multiphoton excitation

KW - NIR-ultrafast laser pulses

KW - PDD

KW - PDT

UR - http://www.scopus.com/inward/record.url?scp=105004321667&partnerID=8YFLogxK

U2 - 10.1117/12.3045327

DO - 10.1117/12.3045327

M3 - 会議への寄与

AN - SCOPUS:105004321667

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Methods for Tumor Treatment and Detection

A2 - Kessel, David H.

A2 - Hasan, Tayyaba

A2 - Maytin, Edward V.

PB - SPIE

T2 - Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII 2025

Y2 - 25 January 2025 through 27 January 2025

ER -

Yashiki S, Ogawa K, Shiroshita H, Inomata M, Katagiri T , Oshima Y. In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA. In Kessel DH, Hasan T, Hasan T, Hasan T, Maytin EV, editors, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXIII. SPIE. 2025. 1329903. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.3045327

In vitro and in vivo analysis in NIR-PDD combining multiphoton excitation and 5-ALA

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Fingerprint

Cite this